Skip to main content
SpringerLink
Log in

Evidence for gametoclonal variation in potato (Solanum tuberosum L.)

  • Original paper
  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

Gametoclonal variation that occurs in gametic cells in culture and is recovered in their regenerated derivatives has not been reported in potato (Solanum tuberosum L.). Based on a set of 24 differentiating phenotypic traits, canonical variates analysis genetically distinguished the androgenic (di)haploid (2n = 2x = 24) D4 from its tetraploid (2n = 4x = 48) anther-derived sibs and anther donor JTH/C-107. Nuclear microsatellite analysis over six polymorphic loci indicated that meiotic rearrangements and mutant alleles were primarily associated with the release of gametoclonal variation. The incidence of null alleles in D4 at the loci STACCAS3 and STM0031 was also indicative of mutations occurring within the priming sequence. Microsatellite results were supported by random amplified polymorphic DNA (RAPD) assays that characterized a total of 567 loci (bins) representing 4,258 amplified fragments. Sixty-five new RAPDs that were absent in the anther donor and in either of its parents, viz., S. phureja Juz. & Buk. IVP-35 and S. tuberosum cv. Kufri Jyoti were present in D4, indicating the occurrence of extensive recombinational events. The results have been discussed in the context of microsatellite null alleles providing the most conclusive evidence for gametoclonal variation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Abbreviations

CVA:

Canonical variates analysis

PCR:

Polymerase chain reaction

RAPD:

Random amplified polymorphic DNA

SSR:

Simple sequence repeat

UPGMA:

Unweighted pair-group method arithmetic average

References

  • Bastiaanssen HJM, Ramanna MS, Sawor Z, Mincione A, Steen AVD, Jacobsen E (1996) Pollen markers for gene centromere mapping in diploid potato. Theor Appl Genet 93:1040–1047

    Article  CAS  Google Scholar 

  • Bentolila S, Hardy T, Guitton C, Freyssinet G (1992) Comparative genetic analyses of F2 plants and anther culture derived plants of maize. Genome 35:575–582

    Google Scholar 

  • Callen DF, Thompson AD, Shen Y, Phillips HA, Richards RI, Mulley JC, Sutherland GR (1993) Incidence and origin of “null” alleles in the (AC)n microsatellite markers. Am J Hum Genet 52:922–927

    CAS  PubMed  Google Scholar 

  • Cappadocia M, Ramulu KS (1998) Anther culture in potato improvement. In: Khurana SMP, Chandra R, Upadhya M (eds) Comprehensive potato biotechnology. Malhotra Publishing House, New Delhi, pp 1–30

    Google Scholar 

  • Chani E, Veilleux RE, Boluarte-Medina T (2000) Improved androgenesis of interspecific potato and efficiency of SSR markers to identify homozygous regenerants. Plant Cell Tissue Organ Cult 60:101–112

    Article  CAS  Google Scholar 

  • Chani E, Ashkenazi V, Hillel J, Veilleux RE (2002) Microsatellite marker analysis of an anther-derived potato family: skewed segregation and gene-centromere mapping. Genome 45:236–242

    Article  CAS  PubMed  Google Scholar 

  • Doggramac-Altuntepe M, Peterson TS, Jauhar PP (2001) Anther culture derived regenerants of durum wheat and their cytological characterization. J Hered 92:56–64

    Article  Google Scholar 

  • Evans DA, Sharp WR, Medina-Filho HP (1984) Somaclonal and gametoclonal variation. Am J Bot 71:759–774

    Article  Google Scholar 

  • Finnie SJ, Forster BP, Chalmers KJ, Dyer AF, Waugh R, Powell W (1991) Genetic stability of microspore-derived doubled haploids of barley: a cytological, biochemical, and molecular study. Genome 34:923–928

    CAS  Google Scholar 

  • Huang B (1996) Gametoclonal variation in crop improvement. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants, vol 2. Kluwer, Dordrecht, pp 73–91

    Google Scholar 

  • Javornik B, Bohanec B, Campion B (1998) Second cycle gynogenesis in onion, Allium cepa L., and genetic analysis of the plants. Plant Breed 117:275–278

    Article  Google Scholar 

  • Kinoshita T, Mori K (1998) Somaclonal selection of physiological mutants and several problems related to rice cell breeding. J Appl Genet 39:9–35

    Google Scholar 

  • Kumar A (1994) Somaclonal variation. In: Bradshaw JE, Mackay GR (eds) Potato genetics. CAB International, Wallingford, pp 197–212

    Google Scholar 

  • Link W, Dixkens C, Singh M, Schwall M, Melchinger AE (1995) Genetic diversity in European and Mediterranean faba bean germplasm revealed by RAPD markers. Theor Appl Genet 90:27–32

    Article  CAS  Google Scholar 

  • Logue SJ (1996) Genetic stability in microspore-derived doubled haploids. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants, vol 2. Kluwer, Dordrecht, pp 1–51

    Google Scholar 

  • Lough RC, Varrieur JM, Veilleux RE (2001) Selection inherent in monoploid derivation mechanisms for potato. Theor Appl Genet 103:178–184

    Article  CAS  Google Scholar 

  • Luo ZW, Zhang RM, Kearsey MJ (2004) Theoretical basis for genetic linkage analysis in autotetraploid species. Proc Natl Acad Sci USA 101:7040–7045

    Article  CAS  PubMed  Google Scholar 

  • Meyer R, Salamini F, Uhrig H (1993) Isolation and characterization of potato diploid clones generating a high frequency of monohaploid or homozygous diploid androgenetic plants. Theor Appl Genet 85:905–912

    Article  Google Scholar 

  • Mifidabadi AJ, Jorabchi A, Shahrzad S, Mahmodi F (2001) New genotypes development of Populus euphratica Oliv. using gametoclonal variation. Silvae Genet 50:275–279

    Google Scholar 

  • Munoz M, Cistue L, Castillo AM, Romagosa I, Valles MP (2004) A retrotransposon sequence is related to DNA instability in barley microspore culture. In: Vollmann J, Grausgruber H, Ruckenbauer P (eds) Genetic variation for plant breeding. EUCARPIA General Congress, Tulln, pp 223–225

    Google Scholar 

  • Naik PS, Sarkar D, Gaur PC (1998) Yield components of potato microtubers: in vitro production and field performance. Ann Appl Biol 133:91–99

    Article  Google Scholar 

  • Raker C, Spooner DM (2002) The Chilean tetraploid cultivated potato, Solanum tuberosum, is distinct from the Andean populations; microsatellite data. Crop Sci 42:1451–1458

    Article  Google Scholar 

  • Redha A, Schmid JE, Buter B, Stamp P, Attia T (1998) Cytological investigation of R1 and R2-generations of spontaneously and colchicine induced diploid anther derived wheat plants. Cytologia 63:267–278

    Google Scholar 

  • Rivard SR, Saba-El-Leil MK, Landry BS, Cappadocia M (1994) RFLP analyses and segregation of molecular markers in plants produced by in vitro anther culture, selfing, and reciprocal crosses of two lines of self-incompatible Solanum chacoense. Genome 37:775–783

    CAS  PubMed  Google Scholar 

  • Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal locations, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018

    Article  CAS  PubMed  Google Scholar 

  • Sanguinetti CJ, Dias Neto E, Simpson AJG (1994) Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. Biotechniques 17:915–919

    Google Scholar 

  • Sarkar D, Pandey SK, Sud KC, Chanemougasoundharam A (2004) In vitro characterization of manganese toxicity in relation to phosphorus nutrition in potato (Solanum tuberosum L.). Plant Sci 167:977–986

    Article  CAS  Google Scholar 

  • Schweiger G (1990) Charakterisierung und RFLP-analyse spezifischer Genomkomponenten bei Solanum und ihr Einsatz zur Identifikation somatischer Hybride von S. tuberosum- Zuchtlinien. Dissertation, Eberhard Karls Universität Tübingen

  • Sharma S, Chanemougasoundharam A, Sarkar D, Pandey SK (2004) Carboxylic acids affect induction, development and quality of potato (Solanum tuberosum L.) microtubers grown in vitro from single-node explants. Plant Growth Regul 44:219–229

    Article  CAS  Google Scholar 

  • Sharma S, Sarkar D, Pandey SK (2010) Phenotypic characterization and nuclear microsatellite analysis reveal genomic changes and rearrangements underlying androgenesis in tetraploid potatoes (Solanum tuberosum L.). Euphytica 171:313–326

    Article  CAS  Google Scholar 

  • Sotirova V, Shtereva L, Zagorska N, Dimitrov B, Bogatsevska N (1999) Resistance responses of plants regenerated from tomato anther and somatic tissue cultures to Clavibacter michiganense subsp. michiganense. Israel J Plant Sci 47:237–243

    Google Scholar 

  • Torjek O, Kiss E, Mazik-Tokei K, Hutvagner G, Silhavy D, Banfalvi Z, Kertesz Z, Pank J, Heszky L (2001) Comparative molecular analysis of winter wheat cultivars and their doubled haploid derivatives. Cereal Res Commun 29:41–48

    CAS  Google Scholar 

  • Valdes AM, Slatkin M, Freimer NB (1993) Allele frequencies at microsatellite loci: the stepwise mutation model revisited. Genetics 133:737–749

    CAS  PubMed  Google Scholar 

  • Van de Peer Y, De Watcher R (1994) TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Applic Biosci 10:569–570

    Google Scholar 

  • Veilleux RE (1998) Gametoclonal variation in crop plants. In: Jain SM, Brar DS, Ahloowalia BS (eds) Somaclonal variation and induced mutations in crop improvement. Kluwer, Dordrecht, pp 123–133

    Google Scholar 

  • Veilleux RE, Shen LY, Paz MM (1995) Analysis of the genetic composition of anther-derived potato by random amplified polymorphic DNA and simple sequence repeats. Genome 38:1153–1162

    CAS  PubMed  Google Scholar 

  • Yamagishi M (2002) Gametoclonal variation in anther culture-derived rice plants. II Segregation of mutated plants at the first progeny generation. J Genet Breed 56:303–308

    Google Scholar 

  • Yoshida S, Watanabe K, Fujino M (1998) Non-random gametoclonal variation in rice regenerants from callus subcultured for a prolonged period under high osmotic stress. Euphytica 104:87–94

    Article  Google Scholar 

  • Zagorska NA, Shtereva LA, Kruleva MM, Sotirova VG, Baralieva DL, Dimitrov BD (2004) Induced androgenesis in tomato (Lycopersicon esculentum Mill.). III Characterization of the regenerants. Plant Cell Rep 22:449–456

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We thank Sheesh Ram Thakur and Ram Dayal for field management, and Indian Council of Agricultural Research (ICAR), New Delhi for financial support in the form of an ad-hoc research project (F. No. 8-45/2004-Hort. II). Comments and suggestions on the manuscript from the Editor/an anonymous reviewer are gratefully acknowledged.

Author information

Authors and Affiliations

  1. Cell and Molecular Biology Laboratory, Division of Crop Improvement, Central Potato Research Institute (CPRI), Shimla, Himachal Pradesh, 171001, India

    Debabrata Sarkar, Sushruti Sharma, Poonam Chandel & Suman Kumar Pandey

  2. Biotechnology Unit, Division of Crop Improvement, Central Research Institute for Jute & Allied Fibres (CRIJAF), Barrackpore, Kolkata, West Bengal, 700120, India

    Debabrata Sarkar

Authors
  1. Debabrata Sarkar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sushruti Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Poonam Chandel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Suman Kumar Pandey
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Debabrata Sarkar.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PDF 30 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sarkar, D., Sharma, S., Chandel, P. et al. Evidence for gametoclonal variation in potato (Solanum tuberosum L.). Plant Growth Regul 61, 109–117 (2010). https://doi.org/10.1007/s10725-010-9455-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10725-010-9455-z

Keywords

Search

Navigation