Skip to main content
SpringerLink
Log in

Promotion of somatic embryo production from embryogenic calluses of monocotyledonous and dicotyledonous plants by heavy-ion beam irradiation

  • Brief communication
  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

Effect of heavy-ion beam irradiation on the growth and development of embryogenic calluses was examined in the liliaceous monocotyledon Tricyrtis hirta and the umbelliferous dicotyledon Daucus carota. Embryogenic calluses of T. hirta were irradiated with 5, 10, 20 or 50 Gy of 12C6+, 14N7+ or 20Ne10+ ions, and those of D. carota were irradiated with 5, 10, 20 or 50 Gy of 14N7+ ions. In both species, irradiation at 10–50 Gy inhibited growth of embryogenic calluses, and callus growth rate decreased as irradiation dose increased. Interestingly irradiation at low doses greatly promoted somatic embryo production from embryogenic calluses in both species. In T. hirta, calluses irradiated with 5 and 10 Gy 12C6+ ions, 10 Gy 14N7+ ions, and 5 Gy 20Ne10+ ions produced more than twice as many embryos as the control, non-irradiated calluses. In D. carota, embryogenic calluses irradiated with 5 Gy 14N7+ ions produced more than one and a half times as many embryos as the control. Somatic embryo production in both species was inhibited by irradiation at 20 and 50 Gy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  • Abe T, Bae CH, Ozaki T, Wang JM, Yoshida S (2000) Stress-tolerant mutants induced by heavy-ion beams. Gamma Field Symp 39:45–56

    Google Scholar 

  • Al-Safadi B, Simon PW (1990) The effects of gamma irradiation on the growth and cytology of carrot (Daucus carota L.) tissue culture. Environ Exp Bot 30:361–371

    Article  Google Scholar 

  • Encheva J, Ivanov P, Tsvetkova F, Nikolova V (1993) Development of a new initial breeding material in sunflower (Helianthus annuus L.) using direct organogenesis and somatic embryogenesis. Euphytica 68:181–185

    Article  Google Scholar 

  • Gaj MD (2004) Factors influencing somatic embryogenesis induction and plant regeneration with particular reference to Arabidopsis thaliana (L.) Heynh. Plant Growth Regul 43:27–47

    Article  CAS  Google Scholar 

  • Godo T, Okuno H, Saito H, Miyazawa Y, Ryuto H, Fukunishi N, Abe T (2007) Effects of ion beam irradiation on survival and mutation induction of triploid Senno (Lychnis senno Siebold et Zucc.). Bull Bot Gard Toyama 12:41–46

    Google Scholar 

  • Iqbal MCM, Möllers C, Röbbelen G (1994) Increased embryogenesis after colchicine treatment of microspore cultures of Brassica napus L. J Plant Physiol 143:222–226

    CAS  Google Scholar 

  • Ishii K, Yamada Y, Hase Y, Shikazono N, Tanaka A (2003) RAPD analysis of mutants obtained by ion beam irradiation to hinoki cypress shoot primordia. Nucl Instr Met Phys Res B 206:570–573

    Article  CAS  Google Scholar 

  • Kanaya T, Saito H, Hayashi Y, Fukunishi N, Ryuto H, Miyazaki K, Kusumi T, Abe T, Suzuki K (2008) Heavy-ion beam-induced sterile mutants of verbena (Verbena × hybrida) with an improved flowering habit. Plant Biotechnol 25:91–96

    Google Scholar 

  • MacDonald MV, Aslam FN (1986) The effect of gamma irradiation on buds of Brassica napus ssp. oleifera prior to anther culture. Crucif Newslett 11:90

    Google Scholar 

  • Miyazaki K, Suzuki K, Abe T, Katsumoto Y, Yoshida S, Kusumi T (2002) Isolation of variegated mutants of Petunia hybrida using heavy ion beam irradiation. RIKEN Accel Prog Rep 35:130

    Google Scholar 

  • Miyazaki K, Suzuki K, Iwaki K, Kusumi T, Abe T, Yoshida S, Fukui H (2006) Flower pigment mutations induced by heavy ion beam irradiation in an interspecific hybrid of Torenia. Plant Biotechnol 23:163–167

    CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Muthusamy A, Vasanth K, Sivasankari D, Chandrasekar BR, Jayabalan N (2007) Effects of mutagens on somatic embryogenesis and plant regeneration in groundnut. Biol Plant 51:430–435

    Article  CAS  Google Scholar 

  • Nakano M, Mizunashi K, Tanaka S, Godo T, Nakata M, Saito H (2004) Somatic embryogenesis and plant regeneration from callus cultures of several species in the genus Tricyrtis. In Vitro Cell Dev Biol Plant 40:274–278

    Article  CAS  Google Scholar 

  • Okamura M, Yasuno N, Ohtsuka M, Tanaka A, Shikazono N, Hase Y (2003) Wide variety of flower-color and -shape mutants regenerated from leaf cultures irradiated with ion beams. Nucl Instr Met Phys Res B 206:574–578

    Article  CAS  Google Scholar 

  • Pius J, George L, Eapen S, Rao PS (1994) Evaluation of somaclonal and mutagen induced variation in fingermillet. Plant Breed 112:239–243

    Article  Google Scholar 

  • Puigderrajols P, Jofe A, Mir G, Pla M, Verdaguer D, Huguet G, Molinas M (2002) Developmentally and stress-induced small heat shock proteins in cork oak somatic embryos. J Exp Bot 373:1445–1452

    Article  Google Scholar 

  • Rey HY, Sansberro PA, Collavino MM, Davina JR, Gonzales AM, Mroginski LA (2002) Colchicine, trifluralin, and oryzalin promoted development of somatic embryos in Ilex paraguariensis (Aquifoliaceae). Euphytica 123:49–56

    Article  CAS  Google Scholar 

  • Sasaki K, Aida R, Niki T, Yamaguchi H, Narumi T, Nishijima T, Hayashi Y, Ryuto H, Fukunishi N, Abe T, Ohtsubo N (2008) High-efficiency improvement of transgenic torenia flowers by ion beam irradiation. Plant Biotechnol 25:81–89

    CAS  Google Scholar 

  • Sugiyama M, Saito H, Ichida H, Hayashi Y, Ryuto H, Fukunishi N, Terakawa T, Abe T (2008) Biological effects of heavy-ion beam irradiation on cyclamen. Plant Biotechnol 25:101–104

    Google Scholar 

  • Tanaka A (1999) Mutation induction by ion beams in Arabidopsis. Gamma Field Symp 38:19–28

    Google Scholar 

  • Yamaguchi H, Shimizu A, Hase Y, Degi K, Tanaka A, Morishita T (2008) Mutation induction with ion beam irradiation of lateral buds of chrysanthemum and analysis of chimeric structure of induced mutants. Euphytica 165:97–103

    Article  Google Scholar 

  • Zhou LB, Li WJ, Ma S, Dong XC, Yu LX, Li Q, Zhou GM, Gao QX (2006) Effects of ion beam irradiation on adventitious shoot regeneration from in vitro leaf explants of Saintpaulia ionahta. Nucl Instr Met Phys Res B 244:349–353

    Article  CAS  Google Scholar 

  • Zimmerman L (1993) Somatic embryogenesis: a model for early development in higher plants. Plant Cell 5:1411–1423

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This experiment was performed at RIBF operated by RIKEN Nishina Center and the Center for Nuclear Study, University of Tokyo. This work was partly supported by a research grant for the study on genesis of matter from Ministry of Education, Culture, Sports, Science and Technology of Japan.

Author information

Author notes

  1. Yusuke Watanabe & Toshikazu Nomizu

    Present address: Niigata Agricultural Research Institute, Horticultural Research Center, 177 Mano, Seiro, 957-0111, Japan

  2. Shiro Mori

    Present address: Department of Agro-Environmental Science, Takushoku University Hokkaido Junior College, 4558 Menu, Fukagawa, 074-8585, Japan

  3. Hiromichi Ryuto

    Present address: Faculty of Engineering, Kyoto University, Kyoto Daigaku-Katsura, Nishikyo-ku, Kyoto, 615-8530, Japan

Authors and Affiliations

  1. Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, Niigata, 950-2181, Japan

    Masaru Nakano, Yusuke Watanabe, Toshikazu Nomizu, Mami Suzuki, Keiko Mizunashi, Shiro Mori, Junji Amano & Dong-Sheng Han

  2. Radiation Biology Team, Nishina Accelerator Research Center, RIKEN, 2-1 Hirosawa, Wako, 351-0198, Japan

    Hiroyuki Saito & Tomoko Abe

  3. Beam Technology Team, Nishina Accelerator Research Center, RIKEN, 2-1 Hirosawa, Wako, 351-0198, Japan

    Hiromichi Ryuto & Nobuhisa Fukunishi

Authors
  1. Masaru Nakano
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yusuke Watanabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Toshikazu Nomizu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mami Suzuki
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Keiko Mizunashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shiro Mori
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Junji Amano
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Dong-Sheng Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Hiroyuki Saito
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Hiromichi Ryuto
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Nobuhisa Fukunishi
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Tomoko Abe
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Masaru Nakano.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nakano, M., Watanabe, Y., Nomizu, T. et al. Promotion of somatic embryo production from embryogenic calluses of monocotyledonous and dicotyledonous plants by heavy-ion beam irradiation. Plant Growth Regul 60, 169–173 (2010). https://doi.org/10.1007/s10725-009-9438-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10725-009-9438-0

Keywords

Search

Navigation