Abstract
Barley (Hordeum vulgare L.) seeds were soaked in aqueous 10−4 M dihydroquercetin (DHQ) to examine its influence on seed germination and further growth of seedlings under optimal soil watering and flooding conditions. The adaptive potential of the plants was estimated by the content of thiobarbituric acid reactive substances (TBARs) and the activity of ascorbate peroxidase (AsP). High-grade seeds were germinated evenly under (−DHQ)- and (+DHQ)-treatments. Low-grade seeds soaked in DHQ, showed no mold and twofold germination rate in comparison with the same seeds soaked in water. The seedlings grown from the similarly germinated seeds did not differ from each other in the shoot growth, independent of the DHQ-pretreatment. The root growth was higher in DHQ-pretreated plants. Soil flooding suppressed the shoot and root growth rates in non-pretreated and DHQ-pretreated plants, however TBARs content was lower in the roots and leaves of (+DHQ)-seedlings as compared to the (−DHQ)-ones. The activity of AsP increased more significantly in the (+DHQ)-plants. The ratio between TBARs content and the AsP activity was lower in the leaves of (+DHQ)-plants both under optimal soil conditions and flooding. Thus, the treatment of low-grade barley seeds with DHQ protects the seeds against mold and increases adaptive potential of the seedlings.
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References
Asada K (2006) Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiol 141:391–396. doi:10.1104/pp.106.082040
Balakhnina TI, Bennicelli RP, Stępniewska Z et al (2004) Oxygen stress in the root zone and plant response (some examples). In: Józefaciuk G (ed) Physics, chemistry and biogeochemistry in soil and plant studies. Alf-Graf, Lublin, pp 23–26
Baraboy VA (1976) Biologicheskoe deistvie rastitel’nikh fenol’nikh soedinenii (Biological action of plant phenolic compounds). Naukova Dumka, Kiev
Bennicelli RP, Stepniewski W, Zakrzhevsky DA, Balakhnina TI et al (1998) The effect of soil aeration on superoxide dismutase activity, malondialdehyde level, pigment content and stomatal diffusive resistance in maize seedlings. Environ Exp Bot 39:203–211. doi:10.1016/S0098-8472(97)00044-0
Biokhimzashchita (2002) Lariksin-regulator rosta rastenii s fungisidnoi aktivnost’yu k kompleksu gribnikh, bakterial’nikh i virusnikh boleznei narabativaetsya iz drevisini listvennitsy (Larixin as plant growth regulator with fungicidal activity to complex of mycotic, bacterial and viral infections is made from the larch wood). Institute of Cytology and Genetics SB RAS, Technical Certificate 2449-001-55457436-02
Blanke MM, Cooke DT (2004) Effects of flooding and drought on stomatal activity, transpiration, photosynthesis, water potential and water channel activity in strawberry stolons and leaves. Plant Growth Regul 42:153–160. doi:10.1023/B:GROW.0000017489.21970.d4
Brown JE, Khodr H, Hider RC et al (1998) Structural dependence of flavonoid interactions with Cu2+ ions: implications for their antioxidant properties. Biochem J 330:1173–1178
Giannopolitis CN, Ries SK (1977) Superoxide dismutases. I. Occurrence in higher plants. Plant Physiol 59(2):309–314
Glinski J, Stepniewski W (1985) Soil aeration and its role for plants. CRC, Boca Raton
Haraguchi H, Mochida Y, Sakai S et al (1996) Protection against oxidative damage by dihydroflavonols in Engelhardtia chrysolepis. Biosci Biotechnol Biochem 60(6):945–948
Jackson MB (1991) Regulation of water relationships in flooded plants by ABA from leaves, roots and xylem sap. In: Davies WJ, Jones HG (eds) Abscisic acid: physiology and biochemistry. Bios Scientific, Oxford, pp 217–226
Jackson MB, Drew MC (1984) Effects of flooding on growth and metabolism of herbaceous plants. In: Kozlowski TT (ed) Flooding and plant growth. Academic Press, Orlando, Florida, pp 47–128
Kalashnikov YE, Zakrzhevsky DA, Balakhnina TI (1994) Effect of soil hypoxia on activation of oxygen and the system of protection from oxidative destruction in roots and leaves of Hordeum vulgare L. Russ J Plant Physiol 41:583–588
Kandaswami C, Perkins E, Drzewiecki G et al (1992) Differential inhibition of proliferation of human squamous cell carcinoma, gliosarcoma and embryonic fibroblast-like lung cells in culture by plant flavonoids. Anticancer Drugs 3(5):525–530. doi:10.1097/00001813-199210000-00013
Kostyuk VA, Potapovich AI (1998) Antiradical and chelating effects in flavonoid protection against silica-induced cell injury. Arch Biochem Biophys 355(1):43–48. doi:10.1006/abbi.1998.0708
Larson RA (1988) The antioxidants of higher plants. Phytochemistry 27:969–978. doi:10.1016/0031-9422(88)80254-1
Melnikova NB, Yoffe ID (2002) Biosovmestimost’ digidrokvertsetina s lipofil’nym i gidrofil’nym fragmentami biomembrani. Vliyanie ionov metallov i askorbinovoi kisloty (Biocompatibility of dihydroquercetin with lipophilic fragments of biomembrane. Effects of metal ions and ascorbic acid). Khim Rast Syr (Chem Plant Stuff) 2:93–103
Morita J (1988) Inactivation of bacteriophages by polyphenols in the presence of cupric ion. Agric Biol Chem 52(7):1669–1673
Muzafarov EN, Ruzieva RK (1985) Fenol’nye soedineniya kak factory regulyatsii fotosinteza i rosta (Phenolic compounds as a factor of photosynthesis and growth regulation). In: Kefeli VI, Toma SI (eds) Rost rastenii i ego regulyatsiya (Plant growth and its regulation). Shtiintsa, Kishinev, pp 210–218
Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880
Rice-Evans CA, Miller NJ, Paganga G (1997) Antioxidant properties of phenolic compounds. Trends Plant Sci 2(4):152–159. doi:10.1016/S1360-1385(97)01018-2
Teselkin YO, Zhambalova BA, Babenkova I et al (1996) Dihydroquercetin antioxidant properties. Biophysics 41(3):620–624
Trought MCT, Drew MC (1980) The development of waterlogging in wheat seedlings (Triticum aestivum L.). I. Shoot and root growth in relation to changes in the concentrations of dissolved gases and solutes in the soil solution. Plant Soil 54:77–94. doi:10.1007/BF02182001
Uchiyama M, Mihara M (1978) Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 86:287–297. doi:10.1016/0003-2697(78)90342-1
Uminsky AA, Havsteen BH, Bakaneva BF (2007) Biokhimiya flavonoidov i ik znachenie v meditsine (The biochemistry and medical significance of flavonoids). Photon-vek, Pushchino
Yamasaki H, Sakihama Y, Ikehara N (1997) Flavonoid-peroxidase reaction as a detoxification mechanism of plant cells against H2O2. Plant Physiol 115:1405–1412
Yan B, Dai Q, Liu X et al (1996) Flooding-induced membrane damage, lipid oxidation and activated oxygen generation in corn leaves. Plant Soil 179:261–268. doi:10.1007/BF00009336
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Balakhnina, T.I., Gavrilov, A.B., Włodarczyk, T.M. et al. Dihydroquercetin protects barley seeds against mold and increases seedling adaptive potential under soil flooding. Plant Growth Regul 57, 127–135 (2009). https://doi.org/10.1007/s10725-008-9327-y
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DOI: https://doi.org/10.1007/s10725-008-9327-y