Skip to main content
SpringerLink
Log in

Is there crosstalk between extracellular and intracellular calcium mobilization in jasmonic acid signaling

  • Brief Communication
  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

The changes in cytosolic Ca2+ levels play an important role in the jasmonic acid (JA) signal transduction pathway. We demonstrate that an increase in cytosolic free Ca2+ concentration ([Ca2+]cyt) of Arabidopsis leaf cells was affected by pretreatment with heparin and 3,4,5-trimethoxybenzoic acid 8-(diethylamino)octyl ester (TMB-8). With pretreatment of heparin, an antagonist of inositol 1,4,5-trisphosphate (IP3) sensitive channels, the basal and JA induced fluorescence of [Ca2+]cyt were both decreased. Furthermore, heparin and TMB-8, another antagonist of IP3 sensitive channels, enhanced the JA-induced gene expression of JR1. These data suggest that there may be a fine tune control system between extracellular and intracellular Ca2+ mobilization in JA signaling pathway.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  • Baum G, Long JC, Jenkin GI, Trewavas AJ (1999) Stimulation of the blue light phototropic receptor NPH1 causes a transient increase in cytosolic Ca2+. Proc Natl Acad Sci USA 96:13554–13559

    Article  PubMed  CAS  Google Scholar 

  • Blume B, Nürnberger T, Nass N, Scheel D (2000) Receptor-mediated increase in cytoplasmic free calcium required for activation of pathogen defense in parsley. Plant Cell 12:1425–1440

    Article  PubMed  CAS  Google Scholar 

  • Boter M, Ruiz-Rivero O, Abdeen A, Prat S (2004) Conserved MYC transcription factors play a key role in jasmonate signaling both in tomato and Arabidopsis. Genes Dev 18:1577–1591. doi:10.1101/gad.297704

    Article  PubMed  CAS  Google Scholar 

  • Cessna SG, Chandra S, Lows PS (1998) Hypo-osmotic shock of tobacco cells stimulates Ca2+ fluxes deriving first from external and then internal Ca2+ stores. J Biol Chem 273:27286–27291. doi:10.1074/jbc.273.42.27286

    Article  PubMed  CAS  Google Scholar 

  • Farmer EE, Almeras E, Krishnamurthy V (2003) Jasmonates and related oxylipins in plant responses to pathogenesis and herbivory. Curr Opin Plant Biol 6:372–378. doi:10.1016/S1369-5266(03)00045-1

    Article  PubMed  CAS  Google Scholar 

  • Fisahn J, Herde O, Willmitzer L, Pena-Cortes H (2004) Analysis of the transient increase in cytosolic Ca2+ during the action potential of higher plants with high temporal resolution: requirement of Ca2+ transients for induction of jasmonate acid biosynthesis and PINII gene expression. Plant Cell Physiol 45:456–459. doi:10.1093/pcp/pch054

    Article  PubMed  CAS  Google Scholar 

  • Gehring CA, Irving HR, Kabbara AA, Parish RW, Boukli NM, Broughton WJ (1997) Rapid, plateau-like increases in intracellular free calcium are associated with nod-factor—induced root-hair deformation. Mol Plant Microbe Interact 10:791–802. doi:10.1094/MPMI.1997.10.7.791

    Article  CAS  Google Scholar 

  • Gilroy S, Read ND, Trewavas AJ (1990) Elevation of cytoplasmic calcium by caged calcium or caged inositol triphosphate initiates stomatal closure. Nature 346:769–771. doi:10.1038/346769a0

    Article  PubMed  CAS  Google Scholar 

  • Gong M, van der Luit AH, Knight MR, Trewavas AJ (1998) Heat-shock-induced changes in intracellular Ca2+ level in tobacco seedlings in relation to thermotolerance. Plant Physiol 116:429–437. doi:10.1104/pp.116.1.429

    Article  CAS  Google Scholar 

  • Harada A, Sakai T, Okada K (2003) Phot1 and phot2 mediate blue light-induced transient increases in cytosolic Ca2+ differently in Arabidopsis leaves. Proc Natl Acad Sci USA 100:8583–8588

    Article  PubMed  CAS  Google Scholar 

  • Hetherington AH, Brownlee C (2004) The generation of Ca2+ signals in plants. Annu Rev Plant Biol 55:401–427. doi:10.1146/annurev.arplant.55.031903.141624

    Article  PubMed  CAS  Google Scholar 

  • Kenton P, Mur L, Draper J (1999) A requirement for calcium and protein phosphatase in the jasmonate-induced increase in tobacco leaf acid phosphatase specific activity. J Exp Bot 50:1331–1341. doi:10.1093/jexbot/50.337.1331

    Article  CAS  Google Scholar 

  • Knight MR, Campbell AK, Smith SM, Trewavas TA (1991) Transgenic plant aequorin reports the effects of touch and cold-shock and elicitors on cytoplasmic calcium. Nature 352:524–526. doi:10.1038/352524a0

    Article  PubMed  CAS  Google Scholar 

  • Lam CMC, Yeung PKK, Wong JTY (2005) Monitoring cytosolic calcium in the Dinoflagellate Crypthecodinium cohnii with calcium orange-AM. Plant Cell Physiol 46:1021–1027. doi:10.1093/pcp/pci102

    Article  PubMed  CAS  Google Scholar 

  • León J, Rojo E, Sánchez-Serrano JJ (2001) Wounding signaling in plants. J Exp Bot 52:1–9. doi:10.1093/jexbot/52.354.1

    Article  PubMed  Google Scholar 

  • Liu H-T, Li B, Shang Z-L, Li X-Z, Mu R-L, Sun D-Y, Zhou R-G (2003) Calmodulin is involved in heat shock signal transduction in wheat. Plant Physiol 132:1186–1195

    Article  PubMed  CAS  Google Scholar 

  • Lorenzo O, Chico JM, Sánchez-Serrano JJ, Solano R (2004) JASMONATE-INSENSITIVE1 encodes a MYC transcription factor essential to discriminate between different jasmonate-regulated defense responses in Arabidopsis. Plant Cell 16:1938–1950

    Article  PubMed  CAS  Google Scholar 

  • Mason HS, Mullet JE (1990) Expression of two soybean vegetative storage protein genes during development and in response to water deficit, wounding, and jasmonic acid. Plant Cell 2:569–579

    Article  PubMed  CAS  Google Scholar 

  • Pei ZM, Murata Y, Benning G, Thomine S, Klusener B, Allen GJ, Grill E, Schroeder JI (2000) Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells. Nature 406:731–734

    Article  PubMed  CAS  Google Scholar 

  • Sambrook , Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

    Google Scholar 

  • Sanders D, Brownlee C, Harper JF (1999) Communicating with calcium. Plant Cell 11:691–706

    Article  PubMed  CAS  Google Scholar 

  • Sanders D, Pelloux J, Brownlee C, Harper JF (2002) Calcium at the crossroads of signaling. Plant Cell 14:S401–S417

    PubMed  CAS  Google Scholar 

  • Schumaker KS, Sze H (1987) Inositol 1,4,5-trisphosphate releases Ca2+ from vacuolar membrane vesicles of oat roots. J Biol Chem 262:3944–3946

    PubMed  CAS  Google Scholar 

  • Shaw SL, Long SR (2003) Nod factor elicits two separable calcium responses in Medicago truncatula root hair cells. Plant Physiol 131:976–984

    Article  PubMed  CAS  Google Scholar 

  • Sun QP, Guo Y, Sun Y, Sun DY, Wang XJ (2006) Influx of extracellular Ca2+ involved in jasmonic-acid-induced elevation of [Ca2+]cyt and JR1 expression in Arabidopsis thaliana. J Plant Res 119:343–350

    Article  PubMed  CAS  Google Scholar 

  • Sun QP, Wang X (2004) The detection of cytosolic free Ca2+ in cells of Arabidopsis thaliana leaves. J Trop Subtrop Bot 12:79–82

    CAS  Google Scholar 

  • Titarenko E, Rojo E, León J, Sánchez-Serrano JJ (1997) Jasmonic acid-dependent and -independent signaling pathways control wound-induced gene activation in Arabidopsis thaliana. Plant Physiol 115:817–826

    Article  PubMed  CAS  Google Scholar 

  • Wood NT, Allan AC, Haley A, Viry-Moussaid M, Trewavas TA (2000) The characterization of differential calcium signaling in tobacco guard cells. Plant J 24:335–344

    Article  PubMed  CAS  Google Scholar 

  • Xiong L, Schumaker KS, Zhu J-K (2002) Cell signaling during cold, drought, and salt stress. Plant Cell 14:S165–S183

    Article  PubMed  CAS  Google Scholar 

  • Zhang W-H, Rengel Z, Kuo J (1998) Determination of intracellular Ca2+ in cells of intact wheat roots: loading of acetoxymethyl ester of Fluo-3 under low temperature. Plant J 15:147–151

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by National Natural Science Foundation of China (grant no. 30700428), Beijing Natural Science Foundation (grant no. 5072009) and The New Star Plan of Science and Technology item of Beijing (grant no. 2006B26).

Author information

Authors and Affiliations

  1. Department of Biotechnology, Beijing Agriculture College, Beijing, 102206, People’s Republic of China

    Qing-peng Sun, Shan-xia Wan, Fu-kuan Zhao & Yu-lan Hao

  2. Center of Tissue Culture, Beijing Agriculture College, Beijing, 102206, People’s Republic of China

    Yong-kun Yu

Authors
  1. Qing-peng Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yong-kun Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shan-xia Wan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fu-kuan Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yu-lan Hao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Qing-peng Sun.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sun, Qp., Yu, Yk., Wan, Sx. et al. Is there crosstalk between extracellular and intracellular calcium mobilization in jasmonic acid signaling. Plant Growth Regul 57, 7–13 (2009). https://doi.org/10.1007/s10725-008-9317-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10725-008-9317-0

Keywords

Search

Navigation