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Morphological and molecular characterization revealed high species level diversity among cultivated, introduced and wild roses (Rosa sp.) of western Himalayan region

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Abstract

High level of genetic diversity and morphological variations were observed among and within the different Rosa species studied. The key traits which differentiated the species were nature of plant, habit, shape of prickles, shape of stipules, leaflet shape, type of leaf margin, vestiture of ventral side of leaflet and flower shape, whereas, traits for distinguishing the accessions within a species (as in case of accessions from western Himalayan region) were flower colour, petal number, shape of hip and hip colour. Some of the accessions from western Himalayan region formed out-groups (IHBT-WR-18, IHBT-WR-21, IHBT-WR-31and IHBT-WR-43B) and could not be classified at the species level. High degree of variations among the accessions of R. multiflora group as compared to variations among the accessions of R. moschata, R. brunonii and R. cathayensis, suggests high level of out-breeding in R. multiflora which may contribute to further variations. Desirable variations were observed for flower colour, petal number, lack of prickles, perpetual flowering, large flower diameter and large fruit size which contribute significantly to diversity of the roses and have potential use in breeding programs.

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References

  • Ambasta SP (1986) The useful plants of India. Publication and Information Directorate CSIR, New Delhi, pp 528–530

    Google Scholar 

  • Babaei A, Tabaei-Aghdaei SR, Khosh-Khui M et al (2007) Microsatellite analysis of Damask rose (Rosa damascena Mill.) accessions from various regions reveals multiple genotypes. BMC Plant Biol 10:7–12

    Google Scholar 

  • Bailey LH (1947) The standard cyclopedia of horticulture, vol 3. The Mac Millian Co., New York, pp 2081–2098

    Google Scholar 

  • Bailey LH (1976) Hortus third. The Mac Millian Co., New York, pp 974–982

    Google Scholar 

  • Baydar GN, Baydar H, Debener T (2004) Analysis of genetic relationships among Rosa damascena plants grown in Turkey by using AFLP and microsatellite markers. J Biotech 111:263–267

    Article  Google Scholar 

  • Bretting PK, Widerlencher MP (1995) Genetic markers and horticultural germplasm management. Hort Sci 30:1349–1356

    Google Scholar 

  • Broertjes C, van Harten AM (1978) Application of mutation breeding methods in the improvement of vegetatively propagated crops. Elsevier, Amsterdam

    Google Scholar 

  • Chowdhery HJ, Wadhwa BM (1984) Flora of Himachal Pradesh analysis, vol 1. B.S.I. Publication, Delhi, pp 252–255

    Google Scholar 

  • Collet H (1984) Flora simlensis. International Book Distributors, Dehra Dun, pp 167–169

    Google Scholar 

  • Cubero JI, Millan T, Osuna F (1994) Varietal identification in Rosa by using isozyme and RAPD markers. Acta Hort 424:261–264

    Google Scholar 

  • Daels-Rakotoarison DA, Gressier B, Trotin F et al (2002) Effects of Rosa canina fruit extract on neutrophil respiratory burst. Phytother Res 16(2):157–161

    Article  CAS  PubMed  Google Scholar 

  • Daysoylu KS, Duman AD, Gezginc Y et al (2005) Rose hips in yoghurt manufacturing. Acta Hortic 690:285–288

    Article  Google Scholar 

  • Dhyani D (2004) Conservation and morphological variability studies in Rosa cathayensis. J Ornam Hortic 7(3–4):97–101

    Google Scholar 

  • Dhyani D, Mukherjee D (2002) Gulabkiekprajati, Rosa moschata ke prakritik prabhedo mein akriti k vibhinnata evam sanrachhan ka adhyayan. BhartiyaVaigyanik evam Audyogik Anusandhan Patrika 8:23–25 (in Hindi)

    Google Scholar 

  • Dhyani D, Singh S (2014) Potential wild rose germplasm of Western Himalayas: conservation, evaluation and registration. Indian J Agric Sci 84(2):53–59

    Google Scholar 

  • Duman AD, Daysoylu KS, Gezginc Y et al (2005) Supplementation of ice cream with different rose hip products. Acta Hortic 690:289–291

    Article  Google Scholar 

  • Emadpour M, Hosseini R, Beiramizadeh E (2009) A high genetic diversity revealed between commercial rose cultivars by RAPD-PCR technique. Int J Plant Prod 3(4):61–66

    CAS  Google Scholar 

  • Ercisli S, Guleryuz M (2005) Rose hip utilization in Turkey. Acta Hortic 690:77–81

    Article  Google Scholar 

  • Fredrick C, Waganer A, Morvillo N (2002) Randomly amplified polymorphic DNA (RAPD) analysis of musk rose (Rosa moschata). Pro Fla State Hort Soc. 115:117–119

    Google Scholar 

  • Gudin S (2000) Rose: genetics and breeding. In: Janick J (ed) Plant breeding reviews, Chapter 17, vol 17. Wiley, New York, pp 159–189

    Google Scholar 

  • Gupta BL (1979) Forest Flora of the Chakrata, Dehra Dun and Saharanpur Forest Division of United Provinces. Jayyed Press, Delhi, pp 220–223

    Google Scholar 

  • Hammer O, Harper DAT, Ryan PD (2001) PAST: Paleontological statistics software package for education and data analysis. Palaeontol Electron 4(1):9

    Google Scholar 

  • Hevia HF, Joublan MJP, Berti DM et al (1999) Date of harvesting musk roses: quality and yield of hips. Agrociencia 15(2):285–289

    Google Scholar 

  • Hooker JD (1879) Flora of British India, vol 2. L. Reev & Co., Ltd., Kent, pp 363–368

    Google Scholar 

  • Joublan JP, Rios D (2005) Rose culture and industry in Chile. Acta-Hort 690:65–69

    Article  Google Scholar 

  • Jurgens AH, Seitz B, Kowarik I (2007) Genetic differentiation of Rosa canina L. at regional and continental scales. Plant Syst Evol 269:39–53

    Article  Google Scholar 

  • Kaur N, Sharma RK, Dhayani D et al (2005) Molecular characterization of inter specific hybrids of scented roses using RAPD markers. In: Fourth international symposium on rose research and cultivation, 18–22 September, Santa Barbara, CA, USA

  • Kiani M, Zamani Z, Khalighi A et al (2007) Wide genetic diversity of Rosa damascena Mill. germplasm in Iran as revealed by RAPD analysis. Sci Hortic 115:386–392

    Article  Google Scholar 

  • Kovacs S, Facsar G, Udvardy L et al (2005) Phenological, morphological and pomological characteristics of some rose species found in Hungary. Acta Hortic 690:71–76

    Article  Google Scholar 

  • Marshall HH (1973) Rose breeding for the prairies. Can Agric 18:24–25

    Google Scholar 

  • Matsumoto S, Fukui H (1996) Identification of rose cultivars and clonal plants by random amplified polymorphic DNA. Sci Hortic 67:49–54

    Article  CAS  Google Scholar 

  • Mohapatra A, Rout G (2005) Identification and analysis of genetic variation among rose cultivars using random amplified polymorphic DNA. Naturforsh 60:611–617

    CAS  Google Scholar 

  • Nair NV, Nair S, Sreenivassan TV et al (1999) Analysis of genetic diversity and phylogeny in Saccharum and related genera using RAPD markers. Genet Resour Crop Evol 46:73–79

    Article  Google Scholar 

  • Naithaini HB (1990) Flowering plants of India, Nepal and Bhutan. Surya Publishers, Dehra Dun, pp 145–146

    Google Scholar 

  • Nwakanma DC, Pillay M, Okoli BE et al (2003) Sectional relationships in the genus Musa L. inferred from the PCR-RFLP of organelle DNA sequences. Theor Appl Genet 107:850–856. doi:10.1007/s00122-003-1340-y

    Article  CAS  PubMed  Google Scholar 

  • Olsson ME, Andersson S, Werlemark G et al (2005) Carotenoids and phenolics in rose hips. Acta-Hort 690:249–252

    Article  CAS  Google Scholar 

  • Özcan M (2002) Nutrient composition of rose (Rosa canina L.) seed and oils. J Med Food 5(3):137–140

    Article  PubMed  Google Scholar 

  • Ozmen I, Ercisli S, Hzarc Y et al (2005) Investigation of antioxidant enzyme activities and lipid peroxidation of Rosa canina and R. dumalis fruits. Acta Hortic 690:245–248

    Article  CAS  Google Scholar 

  • Pal BP (1991) The rose in India. Publication and Information Division, ICAR, New Delhi

    Google Scholar 

  • Peakall R, Smouse PE (2006) Genalex 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295

    Article  Google Scholar 

  • Peakall R, Smouse PE (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research: an update. Bioinformatics 28:2537–2539

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Perrier X, Jacquemoud-Collet JP (2006) Dissimilarity analysis and representation for Windows. http://darwin.cirad.fr/

  • Pirseyedi SM, Mardi M, Davazdahemami S et al (2005) Analysis of the genetic diversity between 12 Iranian Damask rose (Rosa damascena Mill.) using amplified fragment length polymorphism markers. Iran J Biotechnol 3:225–230

    CAS  Google Scholar 

  • Prasad KV, Kumar S, Choudhary ML (2006) Molecular characterisation offragrant rose cultivars. Indian J Hortic 63:229–234

    Google Scholar 

  • Qun G-X, Uggla M, Rumpunen K (2005) Antioxidant activity of dried and boiled rose hips. Acta-Hort 690:239–243

    Google Scholar 

  • Rathore DS, Srivastava UC (1992) Rosa species (A bulletin). NBPGR, Regional station, Phagli, Shimla, India

    Google Scholar 

  • Rehder A (1947) Manual of cultivated trees and shrubs hardy in North America, 2nd edn. MacMillan Co, NY

    Google Scholar 

  • Sabnis TS (1986) Flora of the Punjab and associated hill regions. Scientific Publisher, Jodhpur

    Google Scholar 

  • Shamsizade LA, Novruzov EN (2005) Distribution, fruit properties and productivity of Rosa species in Great Caucasus, Azerbaijan. Acta Hortic 690:101–105

    Article  Google Scholar 

  • Sharma BM, Jamwal PS (1988) Flora of Upper Liddar Valley of Kashmir Himalayas, vol 1. Scientific Publisher, Jodhpur, pp 197–198

    Google Scholar 

  • Tabaei-Aghdaei SR, Monfared HH, Fahimi H et al (2006) Genetic variation analysis of different populations of Rosa damascenain NW Iran using RAPD markers. Iran J Bot 12:121–127

    Google Scholar 

  • Uggla M, Martinsson M (2005) Cultivate the wild roses: experiences from rose hip production in Sweden. Acta Hortic 690:83–89

    Article  Google Scholar 

  • Vos P, Hogers R, Bleeker M et al (1995) AFLP: a new technique for DNA fingerprinting. Nucl Acids Res 23:4407–4414

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Xu Q, Wen X, Deng X (2004) A simple protocol for isolating genomic DNA. From chestnut rose (Rosa roxburghii Tratt) for RFLP and PCR analyses. Plant Mol Biol Rep 22:301a–301g

    Article  Google Scholar 

  • Zieslin N, Moe R (1985) Rosa sp. In: Halevy AH (ed) Handbook of flowering, vol 4. CRC Press, Boca Raton, FL, pp 214–225

    Google Scholar 

Download references

Acknowledgments

We thankfully acknowledge the support of Director, CSIR-Institute of Himalayan Bioresource Technology, Palampur (HP, India) for providing the facilities for the study.

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Authors and Affiliations

  1. CSIR-Institute of Himalayan Bioresource Technology, P.O. Box 6, Palampur, Himachal Pradesh, 176 061, India

    S. Singh, D. Dhyani, A. Nag & R. K. Sharma

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  1. S. Singh
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  2. D. Dhyani
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  3. A. Nag
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  4. R. K. Sharma
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Correspondence to S. Singh.

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Singh, S., Dhyani, D., Nag, A. et al. Morphological and molecular characterization revealed high species level diversity among cultivated, introduced and wild roses (Rosa sp.) of western Himalayan region. Genet Resour Crop Evol 64, 515–530 (2017). https://doi.org/10.1007/s10722-016-0377-0

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