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Peroxidase gene-based estimation of genetic relationships and population structure among Citrus spp. and their relatives

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Abstract

Many stress-related interactions such as pathogen infection, insect tolerance, salt tolerance, auxin degradation, cell wall lignification, tissue suberization, and plant senescence involve various isoforms of peroxidases. Peroxidase gene polymorphism (POGP) markers have been used to estimate diversity, relationships and population structure among 80 Citrus and their relatives in Aurantioideae by using unweighted pair group method arithmetic average (UPGMA) and bayesian substructuring analysis. Fourteen primers produced a total of 148 fragments and 147 of them were polymorphic. The UPGMA analysis demonstrated that the accessions had a similarity range from 0.27 to 0.98 and were distinguished. The results of this study were mostly consistent with previous reports of different marker systems, but few different findings were also detected. The subtribe Clauseninae (tribe Clauseneae) did not clearly separate from the subtribes of the tribe Citreae. Substructuring analysis indicated that there were six subpopulations among the accessions studied. This study revealed that the POGP markers can be utilized to estimate genetic diversity, relationships and population structure in Citrus and related species in the Aurantioideae subfamily.

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References

  • Amaya I, Botella MA, de la Calle M, Medina MI, Heredia A, Bressan RA, Hasegawa PM, Quesada MA, Valpaesta V (1999) Improved germination under osmotic stress of tobacco plants over expressing a cell wall peroxidase. FEBS Lett 457:80–84

    Article  PubMed  CAS  Google Scholar 

  • Barkley NA, Roose ML, Krueger RR, Federici CT (2006) Assessing genetic diversity and population structure in a citrus germplasm collection utilizing simple sequence repeat markers (SSRs). Theor Appl Genet 112:1519–1531

    Article  PubMed  CAS  Google Scholar 

  • Barrett HC, Rhodes AM (1976) A numerical taxonomic study of affinity relationships in cultivated Citrus and its close relatives. Syst Bot 1:105–136

    Article  Google Scholar 

  • Bayer RJ, Mabberley DJ, Morton C, Miller CH, Sharma IK, Pfeil BE, Rich S, Hitchcock R, Sykes S (2009) A molecular phylogeny of the orange subfamily (Rutaceae: Aurantioideae) using nine cpDNA sequences. Am J of Bot 96:668–685

    Article  CAS  Google Scholar 

  • Biswas KM, Chai L, Amar MH, Zhang X, Deng XX (2011) Comparative analysis of genetic diversity in Citrus germplasm collection using AFLP, SSAP, SAMPL and SSR markers. Sci Hort 129:798–803

    Article  CAS  Google Scholar 

  • Ceylan H (2010) Detection of peroxidase gene polymorphism in wheats from Turkey. Erciyes University Institute of Graduate Studies in Science and Engineering, Msc. Thesis, 75 p. (in Turkish)

  • Cristofani-Yaly M, Novelli VM, Bastinel M, Machado MA (2010) Transferability and level of heterozygosity of microsatellite markers in Citrus species. Plant Mol Biol Rep 29:418–423

    Article  Google Scholar 

  • Dice LR (1945) Measures of the amount of ecologic association between species. Ecology 26:297–302

    Article  Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15

    Google Scholar 

  • Espelie KE, Franceschi VR, Kolattukudy PE (1986) Immunocytochemical localization and time course of appearance of an anionic peroxidase associated with suberization in wound-healing potato tuber tissue. Plant Phys 81:487–492

    Article  CAS  Google Scholar 

  • Fang DQ, Krueger RR, Roose ML (1998) Phylogenetic relationships among selected Citrus germplasm accessions revealed by inter-simple sequence repeat (ISSR) markers. J Am Soc Hort 123:612–617

    CAS  Google Scholar 

  • FAO (2011) Food and Agricultural Organization of the United Nations. http://faostat.fao.org/site/567/DesktopDefault.aspx?PageID=567#ancor

  • Federici CT, Fang DQ, Scora RW, Roose ML (1998) Phylogenetic relationships within the genus Citrus (Rutaceae) and related genera as revealed by RFLP and RAPD analysis. Theor Appl Genet 96:812–822

    Article  CAS  Google Scholar 

  • Fukunaga K, Hill J, Vigouroux Y, Matsuoka Y, Sanchez GJ, Liu K, Buckler ES, Doebley J (2005) Genetic diversity and population structure of Teosinte. Genetics 169:2241–2254

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Gulsen O, Roose ML (2000) The origin of Interdonato lemon inferred from cpRFLP, SSR, isozyme and ISSR markers. Proc Int Soc Citricult IX Congr, pp 158–159

  • Gulsen O, Roose ML (2001) Chloroplast and nuclear genome analysis of the parentage of lemons. J Am Soc Hort Sci 126:210–215

    CAS  Google Scholar 

  • Gulsen O, Shearman RC, Heng-Moss TM, Mutlu N, Lee DJ, Sarath G (2007) Peroxidase gene polymorphism in buffalograss and other grasses. Crop Sci 47:767–772

    Article  CAS  Google Scholar 

  • Gulsen O, Sever-Mutlu S, Mutlu N, Tuna M, Karaguzel O, Shearman RC, Riordan TP, Heng-Moss TM (2009) Polyploidy creates higher diversity among Cynodon accessions as assessed by molecular markers. Theor Appl Genet 118:1309–1319

    Article  PubMed  CAS  Google Scholar 

  • Gulsen O, Kaymak S, Ozongun S, Uzun A (2010a) Genetic analysis of Turkish apple germplasm using peroxidase gene-based markers. Sci Hort 125:368–373

    Article  CAS  Google Scholar 

  • Gulsen O, Uzun A, Canan I, Seday U, Canihos E (2010b) A new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers. Euphytica 173:265–277

    Article  CAS  Google Scholar 

  • Herrero R, Asins MJ, Carbonell AE, Navarro L (1996) Genetic diversity in the orange subfamily Aurantioideae. I. Intraspecies and intragenus genetic variability. Theor Appl Genet 92:599–609

    Article  PubMed  CAS  Google Scholar 

  • Hinman RL, Lang J (1965) Peroxidase catalyzed oxidation of indole-3-acetic acid. Biochemistry 4:144–158

    Article  PubMed  CAS  Google Scholar 

  • Hiraga S, Yamamoto K, Ito H, Sasaki K, Matsui H, Honma M, Nagamura Y, Sasaki T, Ohashi Y (2001) Diverse expression profiles of 21 rice peroxidase genes. FEBS Lett 471:245–250

    Google Scholar 

  • Hodgson RW (1967) Horticultural varieties of citrus. In: Reuther W, Webber HJ, Batchelor LD (eds) The citrus industry, vol 1. University of California Press, Berkeley, pp 431–591

    Google Scholar 

  • Jackson LK, Futch SH (2003) Ambersweet Orange. Fact Sheet HS-176, Horticultural Sciences Department, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida

  • Kahn TL, Krueger RR, Gumpf DJ, Roose ML, Arpaia ML, Batkin TA, Bash JA, Bier OJ, Clegg MT, Cockerham ST, Coggins Jr CW, Durling D, Elliott G, Mauk PA, Mcguire PE, Orman C, Qualset CO, Roberts PA, Soost RK, Turco J, Van Gundy SG, Zuckerman B (2001) Citrus genetic resources in California: Analysis and recommendations for long-term conservation. Report No. 22. University of California Division of Agriculture and Natural Resources, Genetic Resources Conservation Program, Davis CA, USA

  • Lombardo G, Schicchi R, Marino P, Palla F (2012) Genetic analysis of Citrus aurantium L. (Rutaceae) cultivars by ISSR molecular markers. Plant Biosyst Int J Deal Asp Plant Biol 146(Sup 1):19–26

    Google Scholar 

  • Lu Z, Zhou Z, Xie R (2011) Molecular phylogeny of the “True Citrus Fruit Trees” group (Aurantioideae, Rutaceae) as inferred from chloroplast DNA sequence. Agric Sci China 10:49–57

    Article  CAS  Google Scholar 

  • Luro F, Rist D, Ollitrault P (2000) Sequence tagged microsatellites polymorphism: an alternative tool for cultivar identification and evaluation of genetic relationships in Citrus. Proc Int Soc Citricult IX Congr 1:170–171

    Google Scholar 

  • Mantel N (1967) The detection of disease clustering and a generalized regression approach. Cancer Res 27:209–220

    PubMed  CAS  Google Scholar 

  • Morton CM (2009) Phylogenetic relationships of the Aurantioideae (Rutaceae) based on the nuclear ribosomal DNA ITS region and three noncoding chloroplast DNA regions, atpB-rbcL spacer, rps16, and trnL-trnF. Org Divers Evol 9:52–68

    Article  Google Scholar 

  • Morton CM, Grant M, Blackmore S (2003) Phylogenic relationships of the Aurantioideae inferred from chloroplast DNA sequence data. Am J Bot 90:1463–1469

    Article  PubMed  CAS  Google Scholar 

  • Nicolosi E, Deng ZN, Gentile A, La Malfa S, Continella G, Tribulato E (2000) Citrus phylogeny and genetic origin of important species as investigated by molecular markers. Theor Appl Genet 100:1155–1166

    Article  CAS  Google Scholar 

  • Ocal N (2012) Determination of genetic diversity in some watermelon cultivars using peroxidase gene markers. Erciyes University Institute of Graduate Studies in Science and Engineering, Msc. Thesis 113 p (in Turkish)

  • Pang XM, Hu CG, Deng XX (2007) Phylogenetic relationship within Citrus and related genera as inferred from AFLP markers. Genet Resour Crop Evol 54:429–436

    Article  CAS  Google Scholar 

  • Passardi F, Cosio C, Penel C, Dunand C (2005) Peroxidases have more functions than a Swiss army knife. Plant Cell Rep 24:255–265

    Article  PubMed  CAS  Google Scholar 

  • Pessina D, Gentili R, Barcaccia G, Nicole S, Rossi G, Barbesti S, Sgorbati S (2011) DNA content, morphometric and molecular marker analyses of Citrus limonimedica, C. limon and C. medica for the determination of their variability and genetic relationships within the genus Citrus. Sci Hort 129:663–673

    Article  CAS  Google Scholar 

  • Pritchard JK, Przeworski M (2001) Linkage disequilibrium in humans: models and data. Am J Hum Genet 69:1–14

    Google Scholar 

  • Rohlf FJ (2000) NTSYS-pc, Numerical Taxonomy and Multivariate Analysis System. Version 2.11. New York, Exeter, Setauket

  • Scora RW (1975) On the history and origin of Citrus. Bull Torrey Bot Club 102:369–375

    Article  Google Scholar 

  • Smith JSC, Chin ECL, Shu H, Smith OS, Wall SJ, Senior ML, Mitchel SE, Kresorich S, Tiegle J (1997) An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): comparisons with data from RFLPs and pedigree. Theor Appl Genet 95:163–173

    Article  CAS  Google Scholar 

  • Swingle WT, Reece PC (1967) The botany of Citrus and its wild relatives. In: Reuther W, Webber HJ, Batchelor LD (eds) The Citrus industry, vol 1., University of California Press, Berkeley, CA, pp 389–390

    Google Scholar 

  • Tanaka T (1977) Fundamental discussion of Citrus classification. Stud Citrol 14:1–6

    Google Scholar 

  • Uzun A, Gulsen O, Kafa G, Seday U (2009a) Field performance and molecular diversification of lemon selections. Sci Hort 120:473–478

    Article  CAS  Google Scholar 

  • Uzun A, Yesiloglu T, Aka-Kacar Y, Tuzcu O, Gulsen O (2009b) Genetic diversity and relationships within Citrus and related genera based on sequence related amplified polymorphism markers (SRAPs). Sci Hort 121:306–312

    Article  CAS  Google Scholar 

  • Uzun A, Gulsen O, Yesiloglu T, Aka-Kacar Y, Tuzcu O (2010) Distinguishing grapefruit and pummelo accessions using ISSR markers. Czech J Genet Plant Breed 46:170–177

    CAS  Google Scholar 

  • Uzun A, Yesiloglu T, Polat I, Aka-Kacar Y, Gulsen O, Yildirim B, Tuzcu O, Tepe S, Canan I, Anil S (2011) Evaluation of genetic diversity in lemons and some of their relatives based on SRAP and SSR markers. Plant Mol Biol Rep 29:693–701

    Article  CAS  Google Scholar 

  • Webber HJ (1967) History and development of the Citrus industry. In: Reuther W, Webber HJ, Batchelor LD (eds) The citrus industry, vol 1. University of California Press, Berkeley, pp 1–39.

  • Xu Q, Chen LL, Ruan X, Chen D, Zhu A, Chen C, Bertrand B, Jiao WB, Hao BH, Lyon MP, Chen J, Gao S, Xing F, Lan H, Chang JW, Ge X, Lei Y, Hu Q, Mia Y, Wang L, Xiao S, Biswas MK, Zeng W, Guo F, Cao H, Yang X, Xu XW, Cheng YJ, Xu J, Liu JH, Luo OJ, Tang Z, Guo WW, Kuang H, Zhang HY, Roose ML, Nagarajan N, Deng XX, Ruan Y (2013) The draft genome of sweet orange (Citrus sinensis). Nat Genet 45:59–68

    Article  PubMed  CAS  Google Scholar 

  • Yamamoto M, Abkenar AA, Matsumoto R, Kubo T, Tominaga S (2008) CMA staining analysis of chromosomes in several species of Aurantioideae. Genet Resour Crop Evol 55:1167–1173

    Article  Google Scholar 

  • Yoshida K, Kaothien P, Matsui T, Kawaoka A, Shinmyo A (2003) Molecular biology and application of plant peroxidase genes. Appl Microbiol Biotech 60:665–670

    Article  CAS  Google Scholar 

  • Zhen-hua L, Zhi-qin Z, Rang-jin X (2011) Molecular phylogeny of the “True Citrus Fruit Trees” group (Aurantioideae, Rutaceae) as inferred from chloroplast DNA sequence. Agr Sci in China 10:49–57

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Horticulture, Erciyes University, Melikgazi, Kayseri, Turkey

    Aydin Uzun & Osman Gulsen

  2. Alata Horticultural Research Station, Erdemli, Mersin, Turkey

    Ubeyit Seday

  3. Department of Horticulture, Cukurova University, Adana, Turkey

    Turgut Yesiloglu & Yildiz Aka Kacar

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  1. Aydin Uzun
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  2. Osman Gulsen
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  3. Ubeyit Seday
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  4. Turgut Yesiloglu
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  5. Yildiz Aka Kacar
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Correspondence to Aydin Uzun.

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Uzun, A., Gulsen, O., Seday, U. et al. Peroxidase gene-based estimation of genetic relationships and population structure among Citrus spp. and their relatives. Genet Resour Crop Evol 61, 1307–1318 (2014). https://doi.org/10.1007/s10722-014-0112-7

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  • DOI: https://doi.org/10.1007/s10722-014-0112-7

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