Abstract
Western Himalayan tea represents the status of Geographical Indication in the form of “Kangra Tea” which is unique to the tea grown world over due to its delicate flavor and high quality. The tea germplasm resources of western Himalayan region include selections from the commercial tea estates, abandoned tea gardens of Kangra valley and elite tea clones of CSIR-IHBT, Palampur. Data were recorded for different morphological traits contributing to yield and the biochemical parameters were evaluated using standard HPLC procedures. Multivariate clustering of the data differentiated the germplasm resources into distinct groups based on comparisons among the clusters for leaf size and biochemical parameters. Leaf size differentiated the tea accessions into six phenotypic groups, while shoot density which is an important yield parameter was observed to be independent of leaf size. On the basis of total catechin and caffeine contents, tea accessions were differentiated into nine groups of which Group I in comparison with other groups, recorded the highest total catechin content, moderate to high caffeine level and high astringency factor (AF). Epicatechin gallate (ECG) had a significantly high correlation with AF, implying that high levels of ECG are critical along with corresponding levels of Epigallocatechin gallate (EGCG) for the production of Theaflavin 3,3′digallate (TFDG) which is an important quality constituent providing astringency and briskness to black tea liquor. Sustainability of tea breeding programme can be achieved through organization of germplasm resources and by utilizing elite tea genotypes in breeding programme.
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References
Anonymous (2008) Minutes of the workshop on status of tea industry in Kangra valley. http://www.ihbt.res.in/TIM/minutes.pdf
Balasaravanan T, Pius PK, Rajkumar R, Muraleedharan N, Shasany AK (2003) Genetic diversity among the south Indian germplasm (Camellia sinensis, C. assamica spp. lasiocalyx) using AFLP markers. Plant Sci 165:365–372
Bald C (1957) Indian Tea—Its culture and manufacture, 6th edn. Logmans, Green and Co., London
Barua DN (1963) Characteristics of the tocklai released clones. Two Bud 10(3):26–28
Barua DN (1989) Science and practice of tea culture. Tea Research Association Calcutta, Jorhat 29–34
Brown AG, Eyton WB, Holmes A, Ollis WD (1969) The identification of the arubigins as polymeric proanthocyanidins. Phytochemistry 8:2333–2340
Chin J, Weng P, Xiaq Y, Xu M, Pei S (2005) Genetic diversity and differentiation of Camellia sinensis L. (cultivated tea) and its wild relatives in Yunnan province of china, revealed by morphology, biochemistry and allozyme studies. Genet Resour Crop Evol 52:41–52
Eden T (1964) Tea, 3rd edn. Logmans, Green and Co., London
FAO (2009) Food and Agiculture Oraganization of the United Nations—Production FAOSTAT
Gerats AM, Martin C (1992) Flavanoid synthesis in petunia hybrida: genetics and molecular biology of flower colour. In: Stafford HA, Ibrahim RK (eds) Phenolic metabolism in plants. Plenum Press, New York, pp 167–175
Gulati A, Rajkumar S, Karthigeyan S et al (2009) Catechin and catechin fractions as biochemical markers to study the diversity of Indian tea (Camellia sinensis (L.) O. Kuntze) germplasm. Chem Biodivers 6:1042–1052
Hajra NG (2001) Advances in selection and breeding of tea—a review. J Plant Crops 29(3):1–17
Hammer O, Harper DAT, Ryan PD (2001) PAST: paleontological statistics software package for education and data analysis. Palaeontol Electron 4(1):9
Harbone JB (1967) Comparative biochemistry of flavonoids. Academic Press, London, pp 226–227
IPGRI (1997) Descriptors for tea (Camellia sinensis). International Plant Genetic Resources Institute, Rome
Karthigeyan S, Rajkumar S, Sharma RK, Gulati A, Sud RK, Ahuja PS (2008) High level of genetic diversity between the selected accessions of tea (Camellia sinensis (L.) O. Kuntze) from abandoned tea gardens in western Himalaya. Biochem Genet 41:810–819
Kottur G, Venkatesan S, Shanmugasundaram R, Kumar S, Murugesan S (2010) Diversity among various forms of catechins and its synthesizing enzyme (phenylalanine ammonia lyase) in relation to quality of black tea (Camellia spp.). J Sci Food Agric 90:1533–1537
Magoma GN, Wachira FN, Obanda M, Imbuga M, Agong SG (2000) The use of catechins as biochemical markers in diversity studies of tea (Camellia sinensis). Genet Resour Crop Evol 47:107–114
Mohanan M, Sharma VS (1981) Morphology and systematics of some tea (Camellia spp.) cultivars. In: Proceedings of the fourth annual symposium on plantation crops, PLACROSYM IV, Central Coffee Research Institute, Chikmagalur 577 117, India, pp 391–400
Obanda M, Owuor P, Taylor SJ (1997) Flavonols composition and caffeine content of green leaf as quality potential indicators of Kenyan black teas. J Sci Food Agric 74:209–215
Owuor PO, Obanda M (1995) Clonal variation in the individual theaflavin levels and their impact on astringency and sensory evaluations. Food Chem 54:273–277
Raina SN, Ahuja PS, Sharma RK et al (2012) Genetic structure and diversity of India hybrid tea. Genet Resour Crop Evol 59:1527–1541
Roberts EAH, Smith RF (1963) Phenolic substances of manufactured tea. II. Spectrophotometric evaluation of tea liquors. J Sci Food Agric 14:689–700
Robertson A (1983) Effects of physical and chemical conditions on the in vitro oxidation of tea catechins. Phytochemistry 22:889–896
Robertson A (1992) The chemistry and biochemistry of black tea production, the non-volatiles. In: Willson KC, Clifford MN (eds) Chapman and Hall, London, pp 603–647
Sanderson GW, Ranadive AS, Eisenberg LS et al (1976) Contribution of phenolic compounds to the taste of tea. Phenolic sulphur and nitrogen in foods and flavours symposium. ACS Symp Ser 26:14–46
Sarma S (1999) Biosynthesis of precursors for liquor and flavor characteristics in black tea. In: Jain NK (ed) Global advances in tea science. Vali Books International (P) Ltd, pp 723–732
Satyanarayana N, Sharma VS (1982) Biometric basis for yield prediction in tea clonal selection. In: Proceeding of PLACROSYM IV, Mysore, India, 3–5 Dec 1981, pp 237–243
Sharma V, Gulati A, Ravindranath SD, Kumar V (2005) A simple and convenient method for analysis of tea biochemicals by reverse phase HPLC. J Food Compost Anal 18:583–594
Sharma RK, Negi MS, Sharma S et al (2010) AFLP-based genetic diversity assessment of commercially important tea germplasm in India. Biochem Genet 48:549–564
Sharma V, Joshi R, Gulati A (2011) Seasonal clonal variations and effects of stresses on quality chemicals and prephenate dehydratase enzyme activity in tea (Camellia sinensis). Eur Food Res Technol 232:307–317
Takeda Y (1994) Differences in caffeine and tannin contents between tea cultivars and application to tea breeding. Jpn Agric Res Q 28:117–123
Takino Y, Imagawa H, Harikawa H, Tanaka A (1964) Studies on the mechanism of the oxidation of tea catechins. Part III. Formation of a reddish orange pigment and its spectral relationship to some benzotropolone derivatives. Agric Biol Chem 28(1):64–71
Venkataramani KS, Sharma VS (1975) Notes on the UPASI tea clones approved by tea board and released for commercial planting. Planters’ Chron 70:119–121
Acknowledgments
We thankfully acknowledge the support of Dr. P. S. Ahuja, Director, CSIR-Institute of Himalayan Bioresource Technology, Palampur (HP, India) for providing the facilities for the study.
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Singh, S., Sud, R.K., Gulati, A. et al. Germplasm appraisal of western Himalayan tea: a breeding strategy for yield and quality improvement. Genet Resour Crop Evol 60, 1501–1513 (2013). https://doi.org/10.1007/s10722-012-9938-z
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DOI: https://doi.org/10.1007/s10722-012-9938-z