Abstract
Microsatellite variation at the nuclear and chloroplast genomes was evaluated for wild European and wild American hops, in order to assess the genetic diversity and origin of cultivated hops. Seven nuclear loci and 32 chloroplast loci were used in the analysis of 182 hop accessions including wild European (68), wild American (48), and cultivars (66). A total of 116 alleles were identified using 7 nuclear microsatellites showing different averages of polymorphism and distribution in the wild American and European accessions and cultivars. Two main groups were established as revealed by several statistical analyses; one including European wild accessions and cultivars and a second group consisting of American wild accessions. Three polymorphic chloroplast microsatellite loci were detected, six alleles were scored which defined a total of five haplotypes that were exclusive or presented different distribution between American and European wild accessions. A major influence of the wild European haplotypes was detected among hop cultivars. To the best of our knowledge, this is the first work reporting the use of chloroplast microsatellites in hops.
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Acknowledgments
This research was supported by funds from the Spanish Ministry of Science and Technology and FEDER (project MCT-02-AGL-02472) and the project CGL2005-06821-C02-01. E.L.P. is in receipt of an F.P.U. grant. The plant material was provided by the USDA-ARS National Clonal Germplasm Repository (Corvallis, OR, USA) supported by CRIS 5358-21000-033-00D and by Branka Javornik from the University of Ljubljana and Slovene Institute of Hop Research and Brewing (Zalec, Slovenia). Thanks are given to the people in the Departamento de Biotecnología (INIA) where part of this research was carried out. Thanks also to Leo Blanco and Fernando Rodríguez for their statistical assistance. The authors want to thank Dr. Jernej Jakse and Fernando Rodríguez for their critical pre-view and comments which helped to improve this paper.
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Peredo, E.L., Ángeles Revilla, M., Reed, B.M. et al. The influence of European and American wild germplasm in hop (Humulus lupulus L.) cultivars. Genet Resour Crop Evol 57, 575–586 (2010). https://doi.org/10.1007/s10722-009-9495-2
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DOI: https://doi.org/10.1007/s10722-009-9495-2