Abstract
A common bean genomic library was constructed using the ‘IAC-UNA’ variety enriched for (CT) and (GT) for microsatellite motifs. From 1,209 sequenced clones, 714 showed microsatellites distributed over 471 simple and 243 compound motifs. GA/CT and GT/CA were the most frequent motifs found among these sequences. A total of 123 microsatellites has been characterized. Out of these, 87 were polymorphic (73.7%), 33 monomorphic (26.8%), and 3 (2.4%) did not amplify at all. In a sample of 20 common bean materials selected from the Agronomic Institute Germplasm Bank, the number of alleles per locus varied 2–9, with an average of 2.82. The polymorphic information content (PIC) of each marker varied from 0.05 to 0.83, with a 0.45 average value. Cluster and principal coordinate analysis of the microsatellite data were consistent with the original assignment of the germplasm accessions into the Andean and Mesoamerican gene pools of common bean. Low polymorphism levels detected could be associated with the domestication process. These microsatellites could be a valuable resource for the bean community because of their use as new markers for genetic studies.
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Acknowledgements
This work was supported by the Foundation for Research for the State of São Paulo (FAPESP), contract 02/03225-9. Dr. A. P. Souza received a fellowship from the National Council of Research and Development (CNPq). Dr. L.L. Benchimol received a post-graduate fellowship (02/00752) and T. Campos received under-graduate (03/13282-2) and post-graduate (140310/2005-3) fellowships from FAPESP and CNPq. We would like to thank Dr. Ange-Marie Risterrucci for helping with the construction of the enriched library and Dr. JP Jacquemoud-Collet for providing Microsat software (CIRAD, France); Dr. Dario A. Palmieri and Dr. Marco A. Takita for helping with the PHRED/CAP3 analysis (IAC, Cordeirópolis, S.P., Brazil) and Dr. Maria I. Zucchi for the bootstrap analysis (IAC, Campinas, S.P., Brazil).
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An erratum to this article can be found at http://dx.doi.org/10.1007/s10722-007-9243-4
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Benchimol, L.L., Campos, T.d., Carbonell, S.A.M. et al. Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of Mesoamerican and Andean origins using new developed microsatellite markers. Genet Resour Crop Evol 54, 1747–1762 (2007). https://doi.org/10.1007/s10722-006-9184-3
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DOI: https://doi.org/10.1007/s10722-006-9184-3