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Glycosaminoglycans and glycolipids as potential biomarkers in lung cancer

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Abstract

In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients. The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different. In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues. In regards to CS/DS, the level of 4S disaccharide (the major component of CS-type A and DS) decreased and the level of 6S disaccharide (the major component of CS- type C) increased in cancer tissues. We also compared the content and structure of GAGs in lung tissues from smoking and non-smoking patients. Analysis of the glycolipids showed all lipids present in these lung tissues, with the exception of sphingomyelin were higher in cancer tissues than in normal tissues. Western analysis showed that syndecan 1 and 2 proteoglycans displayed much higher expression in cancer tissue/biopsy samples. This investigation begins to provide an understanding of patho-physiological roles on glycosaminoglycans and glycolipids and might be useful in identifying potential biomarkers in lung cancer.

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Acknowledgments

This work was supported by National Institutes of Health Grants HL125371, GM38060, HL096972 and HL062244 and HL136271. Research Program was also funded by the Ministry of Education (2015R1D1A1A01060512). Thanks to Yanlei Yu for processing the data from western Western blotting images.

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Authors and Affiliations

  1. Key Laboratory of Marine Drugs, Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, China

    Guoyun Li

  2. Department of Chemistry and Chemical Biology, Biological Sciences, Biomedical Engineering and Chemical and Biological Engineering Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY, 12180, USA

    Guoyun Li, Lingyun Li, Eun Ji Joo, Fuming Zhang & Robert J. Linhardt

  3. College of Medicine, Konyang University, Kongyang Hospital, Daejeon, South Korea

    Ji Woong Son, Young Jin Kim, Jae Ku Kang & Kyung Bok Lee

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  1. Guoyun Li
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Correspondence to Fuming Zhang or Robert J. Linhardt.

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Li, G., Li, L., Joo, E.J. et al. Glycosaminoglycans and glycolipids as potential biomarkers in lung cancer. Glycoconj J 34, 661–669 (2017). https://doi.org/10.1007/s10719-017-9790-7

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  • DOI: https://doi.org/10.1007/s10719-017-9790-7

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