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Inhibition of Helicobacter pylori adhesion to Kato III cells by intact and low molecular weight acharan sulfate

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Abstract

We investigated the inhibitory activity of glycosaminoglycans (GAGs) in terms of growth, adhesion, and VacA vacuolation of Helicobacter pylori. Intact acharan sulfate (AS, MW:114 kDa) potently inhibited H. pylori adhesion to Kato III cells with IC50 value of 1.4 mg/mL, while other GAGs did not show any inhibitory activity except for heparin which is a well-known inhibitor of H. pylori adhesion. To investigate whether low molecular weight acharan sulfate (LMWAS) can inhibit H. pylori adhesion, we performed chemical depolymerization of AS by radical reactions to obtain LMWAS. Its physicochemical properties were characterized by high-performance size exclusion chromatography (HPSEC), agarose gel electrophoresis, disaccharide compositional analysis after digestion with heparinase II, and 1H-NMR spectroscopy. The most potent molecular size of LMWAS was 3 kDa with IC50 value of 32 μg/mL, which is 44-fold more potent than intact AS. These results suggest that AS as well as other GAGs can be chemically depolymerized by free radicals and LMWAS compared to intact AS can be applied as a pharmaceutical candidate in order to inhibit H. pylori adhesion to Kato III cells.

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Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) to YSK (No. 20090083533) and the grant (PJ006652) by National Academy of Agricultural Science, RDA to BSH. We thank Prof. Toshihiko Toida in Chiba University for the interpretation of 1H-NMR results.

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Authors and Affiliations

  1. Department of Agricultural Bio-Resources, National Academy of Agricultural Science, RDA, Suwon, 441-707, South Korea

    Joon-Soo Sim & Bum-Soo Hahn

  2. College of Pharmacy, Natural Products Research Institute, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul, 151–742, South Korea

    Joon-Soo Sim, A-Rang Im & Yeong Shik Kim

  3. College of Pharmacy, Inje University, Gimhae, Gyeongnam, 621–749, South Korea

    Youmie Park

  4. College of Pharmacy, Kyung Hee University, Seoul, 130–701, South Korea

    Ji-Eun Shin, Eun-Ah Bae & Dong-Hyun Kim

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  1. Joon-Soo Sim
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Correspondence to Yeong Shik Kim.

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Sim, JS., Hahn, BS., Im, AR. et al. Inhibition of Helicobacter pylori adhesion to Kato III cells by intact and low molecular weight acharan sulfate. Glycoconj J 28, 411–418 (2011). https://doi.org/10.1007/s10719-011-9340-7

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  • DOI: https://doi.org/10.1007/s10719-011-9340-7

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