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SPR and ITC determination of the kinetics and the thermodynamics of bivalent versus monovalent sugar ligand–lectin interactions

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Abstract

A kinetic study of the interaction of bivalent and monovalent sugar ligands with a lectin was undertaken with the aid of surface plasmon resonance (SPR) method. The study involved a series of bivalent α-d-mannopyranoside containing sugar ligands, with systematic variation in the distance between the sugar ligands. The detailed kinetic studies showed that bivalent ligands underwent a faster association (k on) and a slower dissociation (k off) of the ligand–lectin complexes, in comparison to the monovalent ligand–lectin complexes. The kinetic constants were complemented further by assessing the thermodynamic parameters with the aid of isothermal titration calorimetry (ITC). The initiation of cross-linking of ligand–lectin interactions emerge from the early stages of the complexation. The dynamic light scattering (DLS) and the transmission electron microscopy (TEM) techniques allowed judging the sizes and morphologies of the complex in the solution and solid states, respectively.

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Abbreviations

ITC:

Isothermal titration calorimetry

SPR:

Surface plasmon resonance

CMC:

Critical micellar concentration

DLS:

Dynamic light scattering

TEM:

Transmission electron microscopy

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Acknowledgement

We thank Department of Science and Technology, New Delhi, for a financial support. We thank Professor Venugopal and Mr. Alok for DLS measurements. BNM thanks the Council of Scientific and Industrial Research for a research fellowship.

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Authors and Affiliations

  1. Department of Organic Chemistry, Indian Institute of Science, Bangalore, 560 012, India

    Bandaru Narasimha Murthy & Narayanaswamy Jayaraman

  2. National Institute of Immunology, New Delhi, 110 067, India

    Sharmistha Sinha & Avadhesha Surolia

  3. Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560 012, India

    Shantinath S. Indi

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  1. Bandaru Narasimha Murthy
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  2. Sharmistha Sinha
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  3. Avadhesha Surolia
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  4. Shantinath S. Indi
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  5. Narayanaswamy Jayaraman
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Correspondence to Avadhesha Surolia or Narayanaswamy Jayaraman.

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Murthy, B.N., Sinha, S., Surolia, A. et al. SPR and ITC determination of the kinetics and the thermodynamics of bivalent versus monovalent sugar ligand–lectin interactions. Glycoconj J 25, 313–321 (2008). https://doi.org/10.1007/s10719-007-9076-6

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