Abstract
The plant chloroplast (cp) genome is a highly conserved structure which is beneficial for evolution and systematic research. Currently, numerous complete cp genome sequences have been reported due to high throughput sequencing technology. However, there is no complete chloroplast genome of genus Dodonaea that has been reported before. To better understand the molecular basis of Dodonaea viscosa chloroplast, we used Illumina sequencing technology to sequence its complete genome. The whole length of the cp genome is 159,375 base pairs (bp), with a pair of inverted repeats (IRs) of 27,099 bp separated by a large single copy (LSC) 87,204 bp, and small single copy (SSC) 17,972 bp. The annotation analysis revealed a total of 115 unique genes of which 81 were protein coding, 30 tRNA, and four ribosomal RNA genes. Comparative genome analysis with other closely related Sapindaceae members showed conserved gene order in the inverted and single copy regions. Phylogenetic analysis clustered D. viscosa with other species of Sapindaceae with strong bootstrap support. Finally, a total of 249 SSRs were detected. Moreover, a comparison of the synonymous (Ks) and nonsynonymous (Ka) substitution rates in D. viscosa showed very low values. The availability of cp genome reported here provides a valuable genetic resource for comprehensive further studies in genetic variation, taxonomy and phylogenetic evolution of Sapindaceae family. In addition, SSR markers detected will be used in further phylogeographic and population structure studies of the species in this genus.
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Acknowledgements
The authors thanks Jinming Chen for useful comments on the manuscript. This work was supported by the Sino-Africa Joint Research Center (#Y323771W07 and #SAJC201322) and Special Funds for the Young Scholars of Taxonomy of Chinese Academy of Sciences Grants to Liao Kuo (Grant no. ZSBR-013).
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Supplementary Table S1: Nonsynonymous substitution (Ka), Synonymous substitution (Ks), and Ka/Ks values for individual genes or gene groups (XLSX 21 KB)
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Saina, J.K., Gichira, A.W., Li, ZZ. et al. The complete chloroplast genome sequence of Dodonaea viscosa: comparative and phylogenetic analyses. Genetica 146, 101–113 (2018). https://doi.org/10.1007/s10709-017-0003-x
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DOI: https://doi.org/10.1007/s10709-017-0003-x