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New insights on the origin of B chromosomes in Astyanax scabripinnis obtained by chromosome painting and FISH

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Abstract

Chromosome painting (CP) with a probe of B chromosome obtained by microdissection and fluorescence in situ hybridization (FISH) with probes of As51 satellite DNA, C o t−1 DNA, and 18S and 5S rDNA confirmed sharing of some repetitive DNA but not rDNA between A and B chromosomes in the fish Astyanax scabripinnis. Meiotic analysis revealed a pachytene B chromosome bivalent nearly half the size of its mitotic configuration, suggesting a self-pairing of B chromosome arms. Such an isochromosome nature of somatic B chromosome was further evidenced by CP and FISH. All the findings obtained suggest (i) intraspecific origin of B chromosome, and (ii) evolutionary enrichment of repetitive DNA classes, especially those contained in the C o t−1 and the As51 probes, in B chromosome. However, the precise origin of B chromosome in the present species remains to be elucidated by further molecular cytogenetic analysis because of painting of some A chromosome regions with the B chromosome-derived probe.

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Acknowledgments

The authors are grateful to the Brazilian environmental protection agency IBAMA (Instituto Brasileiro do Meio Ambiente e Recursos Naturais Renováveis) for authorization to catch the specimens in nature (IBAMA/MMA/SISBIO license number 15117). The study was funded by the Brazilian fostering agencies CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico; process number 473448/2007-6), CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; process number 087/2007), FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) and the Araucária Foundation (Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná; process number 17217/2009).

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Authors and Affiliations

  1. Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Av. Carlos Cavalcanti, 4748, Ponta Grossa, PR, 84030-900, Brazil

    Marcelo Ricardo Vicari, Helena Flávia de Mello Pistune, Jonathan Pena Castro, Mara Cristina de Almeida & Roberto Ferreira Artoni

  2. Departamento de Genética e Evolução, Universidade Federal de São Carlos, Via Washington Luiz, km 235, São Carlos, SP, 13565-905, Brazil

    Luiz Antonio Carlos Bertollo & Orlando Moreira-Filho

  3. Facultad de Ciencias, Departamento de Genética, Universidad de Granada, Campus Fuentenueva s/n, 18071, Granada, Spain

    Juan Pedro M. Camacho

Authors
  1. Marcelo Ricardo Vicari
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  2. Helena Flávia de Mello Pistune
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  3. Jonathan Pena Castro
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  4. Mara Cristina de Almeida
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  5. Luiz Antonio Carlos Bertollo
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  6. Orlando Moreira-Filho
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  7. Juan Pedro M. Camacho
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  8. Roberto Ferreira Artoni
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Correspondence to Roberto Ferreira Artoni.

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Vicari, M.R., de Mello Pistune, H.F., Castro, J.P. et al. New insights on the origin of B chromosomes in Astyanax scabripinnis obtained by chromosome painting and FISH. Genetica 139, 1073–1081 (2011). https://doi.org/10.1007/s10709-011-9611-z

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  • DOI: https://doi.org/10.1007/s10709-011-9611-z

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