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Cytogenetic analysis of the Ethiopian fruit fly Dacus ciliatus (Diptera: Tephritidae)

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Abstract

The Ethiopian fruit fly, Dacus ciliatus, is an important pest of cucurbits, which recently invaded the Middle East. The genetics and cytogenetics of D. ciliatus have been scarcely studied. Such information is, however, an essential basis for understanding the biology of insect pests, as well as for the design of modern control strategies. We report here the mitotic karyotype and detailed photographic maps of the salivary gland polytene chromosomes of this species. The mitotic metaphase complement consists of six pairs of chromosomes, including one pair of heteromorphic sex (XX/XY) chromosomes. The heterogametic sex is ascribed to the male. The analysis of the salivary gland polytene complement shows a total number of five long chromosomes (10 polytene arms), which correspond to the five autosomes of the mitotic nuclei, and a heterochromatic mass corresponding to the sex chromosomes. Banding patterns, as well as the most characteristic features and prominent landmarks of each polytene chromosome are presented and discussed. Chromosomal homologies between D. ciliatus and Bactrocera oleae are proposed by comparing chromosome banding patterns and by in situ hybridization of the hsp70 gene.

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Acknowledgments

We thank Ian White (Natural History Museum, UK) and Marc De Meyer (Royal Museum for Central Africa, Belgium) for discussing the phylogenetic relationships of Dacinae.

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  1. I. Kounatidis

    Present address: Laboratory of Genes and Development, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK

Authors and Affiliations

  1. Department of Genetics, Development and Molecular Biology, School of Biology, Faculty of Sciences, Aristotle University of Thessaloniki (AUTH), 54124, Thessaloniki, Greece

    E. Drosopoulou, I. Nakou, I. Kounatidis & P. Mavragani-Tsipidou

  2. Department of Entomology, Agricultural Research Organization, Institute of Plant Protection, Bet-Dagan, Israel

    D. Nestel

  3. Department of Agriculture, Crop Production and Rural Environment, University of Thessaly, Phytokou St., 38446, N. Ionia (Volos), Magnisia, Greece

    N. T. Papadopoulos

  4. Department of Environmental and Natural Resources, University of Ioannina, 30100, Agrinio, Greece

    K. Bourtzis

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  1. E. Drosopoulou
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Drosopoulou, E., Nestel, D., Nakou, I. et al. Cytogenetic analysis of the Ethiopian fruit fly Dacus ciliatus (Diptera: Tephritidae). Genetica 139, 723–732 (2011). https://doi.org/10.1007/s10709-011-9575-z

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