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Molecular diversity and relationships among Cymbidium goeringii cultivars based on inter-simple sequence repeat (ISSR) markers

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Abstract

Spring orchid (Cymbidium goeringii) is a popular flowering plant species. There have been few molecular studies of the genetic diversity and conservation genetics on this species. An assessment of the level of genetic diversity in cultivated spring orchid would facilitate development of the future germplasm conservation for cultivar improvement. In the present study, DNA markers of intersimple sequence repeats (ISSR) were identified and the ISSR fingerprinting technique was used to evaluate genetic diversity in C. goeringii cultivars. Twenty-five ISSR primers were selected to produce a total of 224 ISSR loci for evaluation of the genetic diversity. A wide genetic variation was found in the 50 tested cultivars with Nei’s gene diversity (H = 0.2241) and 93.75% of polymorphic loci. Fifty cultivars were unequivocally distinguished based on ISSR fingerprinting. Cultivar-specific ISSR markers were identified in seven of 50 tested cultivars. Unweighted pair-group mean analysis (UPGMA) and principal coordinates analysis (PCA) grouped them into two clusters: one composed the cultivars mainly from Japan, and the other contained three major subclusters mainly from China. Two Chinese subclusters were generally consistent with horticultural classification, and the third Chinese subcluster contained cultivars from various horticultural groups. Our results suggest that the ISSR technique provides a powerful tool for cultivar identification and establishment of genetic relationships of cultivars in C. goeringii.

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Acknowledgments

This research was funded in part by the National Natural Science Foundation (No. 30670199, 30770185, and 30870180), the Natural Science Foundation of Zhejiang Province (No. X305692 and No. X301406), the Zhijiang Scientific and Technological Program (2006C32016), the Hangzhou Scientific and Technological Program (2005132H06), and Qianjiang Scholar Program. The authors are grateful to Mr. Stephen G. Glover at CFS-NRCan for the critical review on the manuscript.

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Author notes

  1. Jiang-Jie Lu

    Present address: Key Laboratory of Modern Forest Breeding Technology, Zhejiang Forestry College, Linan, 311300, China

Authors and Affiliations

  1. Key Laboratory of Biochemistry and Molecular Biology, Hangzhou Normal University, Hangzhou, 310018, China

    Hui-Zhong Wang, Zhen-Xing Wu, Jiang-Jie Lu & Nong-Nong Shi

  2. College of Food, Biological and Environmental Engineering, Zhejiang Gongshang University, Hangzhou, 310035, China

    Yan Zhao

  3. Zhejiang Orchid Society, Hangzhou, 310018, China

    Zhi-Tao Zhang

  4. Pacific Forestry Centre, Canadian Forest Service, Natural Resources Canada, Victoria, Canada, V8Z 1M5

    Jun-Jun Liu

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  1. Hui-Zhong Wang
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Correspondence to Hui-Zhong Wang or Jun-Jun Liu.

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Wang, HZ., Wu, ZX., Lu, JJ. et al. Molecular diversity and relationships among Cymbidium goeringii cultivars based on inter-simple sequence repeat (ISSR) markers. Genetica 136, 391–399 (2009). https://doi.org/10.1007/s10709-008-9340-0

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