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A striking lack of genetic diversity across the wide-ranging amphibian Gastrophryne carolinensis (Anura: Microhylidae)

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Abstract

We examine phylogeographic structure across a wide-ranging microhylid frog (Gastrophryne carolinensis) using both mitochondrial (mtDNA) and nuclear (AFLP) data. Species with similar ecological characteristics such as large range size, low vagility, or existence across known biogeographic barriers, often are comprised of multiple, cryptic lineages. Surprisingly, our analyses of both portions of the genome show very little phylogeographic or population genetic structure. The family Microhylidae is one of the largest families of anurans with over 60 genera and around 400 species distributed across much of the world (Americas, Asia, Africa, and Madagascar), but very few phylogeographic studies have assessed intraspecific genetic diversity across the mitochondrial and nuclear genomes. Our results suggest that G. carolinensis, one of only three species of microhylid native to the US, has experienced a severe population bottleneck with subsequent range expansion. Comparable molecular data from closely related microhylids, in addition to demographic and ecological analyses, will provide valuable insight into patterns of genetic diversity and the processes driving phylogeographic diversity in these wide-ranging frogs.

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Abbreviations

AFLP:

Amplified fragment length polymorphism

ISSR:

Intersimple sequence repeat

IUCN:

The World Conservation Union

LC:

Species of least concern

m:

Meters

ML:

Maximum-likelihood

mM:

Millimolar

MP:

Maximum parsimony

mtDNA:

Mitochondrial DNA

ng:

Nanograms

NJ:

Neighbor-joining

μl:

Microliter

PCoA:

Principal coordinate analysis

RAPD:

Random amplification of polymorphic DNA

cyt b :

Cytochrome b

ND4:

Nicotinamide adenine dinucleotide dehydrogenase subunit 4

tRNA:

Transfer RNA

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Acknowledgements

We are grateful to the following individuals and institutions for their gracious contribution of tissue samples: Z. Ceviron, C. Austin, and R. Brumfield (Louisiana State University Museum of Natural History), T. LaDuc (Texas Natural History Collections), K. Ashton (Archbold Biological Station), and S. Trauth (Arknsas State University). For field help we thank A. Braswell, S. Trauth and his students, J. Apodaca, C. Winne, J. D. Wilson, M. Watson, M. Welker, C. Cox, and C. Makowsky. W. Holzenagel was instrumental in assisting with genetic analyses, and P. Chippindale assisted with analyses. We also thank two anonymous reviewers for helpful comments on this manuscript. Funding was provided by the National Science Foundation (DEB 0414033 and DBI 04-571) to LJR and the University of Alabama.

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  1. Robert Makowsky

    Present address: Department of Biology, University of Texas at Arlington, P.O. Box 19498, Arlington, TX, 76019, USA

  2. Jason Chesser

    Present address: Department of Biology, University of Mississippi, P.O. Box 1848, University, MS, 38677, USA

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  1. Department of Biological Sciences, University of Alabama, MHB Hall, P.O. Box 870345, Tuscaloosa, AL, 35487, USA

    Robert Makowsky, Jason Chesser & Leslie J. Rissler

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Makowsky, R., Chesser, J. & Rissler, L.J. A striking lack of genetic diversity across the wide-ranging amphibian Gastrophryne carolinensis (Anura: Microhylidae). Genetica 135, 169–183 (2009). https://doi.org/10.1007/s10709-008-9267-5

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  • DOI: https://doi.org/10.1007/s10709-008-9267-5

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