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Two novel elements (CFG1 and PYG1) of Mag lineage of Ty3/Gypsy retrotransposons from Zhikong scallop (Chlamys farreri) and Japanese scallop (Patinopecten yessoensis)

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Abstract

Two novel elements (CFG1 and PYG1) of Mag lineage of Ty3/Gypsy retrotransposons were cloned from Zhikong scallop (Chlamys farreri) and Japanese scallop (Patinopecten yessoensis). The total length of the CFG1 element is 4826 bp, including 5′-LTR (192 bp), the entire ORF (4047 bp) and 3′-LTR (189 bp). The entire ORFs of both CFG1 and PYG1 elements are composed of 1348 aa and do not have any frameshifts. Their closest relative is Jule element from the poeciliid fish (Xiphophorus maculatus). On average, the diploid genome of C. farreri contains approximately 84 copies of CFG1 elements. We summarize the major features of CFG1, PYG1 and other elements of Mag lineage of the Ty3/Gypsy group. mRNA expression of CFG1 element in larvae increases gradually before the gastrulae stage and decreases gradually afterward, whereas in adductor such expression in adductor muscle and digestive gland are lower than those in other tissues. Overall, mRNA expression of CFG1 element in the early larvae is significantly higher than that in adult tissues. In muscle tissue, while the promoter and partial GAG domain of CFG1 element are unmethylated, the partial RT domain is highly methylated. These results suggest that CFG1 expression may be controlled by a post-transcriptional gene silencing mechanism that is associated with coding-region (RT domain) methylation.

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Abbreviations

LTR:

Long terminal repeat

ORF:

Open reading frame

GAG:

Group-specific antigen

POL:

Polyprotein

PR:

Protease

RT:

Reverse transriptase

RH:

RNase H

IN:

Integrase

IR:

Inverted repeat

PBS:

Primer binding site

PPT:

Polypurine tract

UGW:

Universal GenomeWalkerTM

AFLP:

Amplified fragment length polymorphism

NJ:

Neighbor-joining

RT-PCR:

Reverse transcriptase-polymerase chain reaction

IAP:

Intracisternal A particle

PTGS:

Post-transcriptional gene silencing

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Acknowledgements

This work was supported by grants from The National High Technology Research and Development Program of China (2006AA10A408; 20060110A4013), a Specialized Research Fund for the Doctoral Program of Higher Education (20060423015), and “NCET-06-0594”.

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Authors and Affiliations

  1. Lab of Molecular Genetics and Breeding of Mollusk, College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao, 266003, China

    Shi Wang, Zhenmin Bao, Xiaoli Hu, Mingyu Shao, Lingling Zhang & Jingjie Hu

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  1. Shi Wang
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  2. Zhenmin Bao
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  3. Xiaoli Hu
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  4. Mingyu Shao
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  5. Lingling Zhang
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Correspondence to Zhenmin Bao.

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Wang, S., Bao, Z., Hu, X. et al. Two novel elements (CFG1 and PYG1) of Mag lineage of Ty3/Gypsy retrotransposons from Zhikong scallop (Chlamys farreri) and Japanese scallop (Patinopecten yessoensis). Genetica 133, 37–46 (2008). https://doi.org/10.1007/s10709-007-9180-3

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  • DOI: https://doi.org/10.1007/s10709-007-9180-3

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