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Congruence between morphological and molecular markers inferred from the analysis of the intra-morphotype genetic diversity and the spatial structure of Oxalis tuberosa Mol.

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Abstract

Oxalis tuberosa is an important crop cultivated in the highest Andean zones. A germplasm collection is maintained ex situ by CIP, which has developed a morphological markers system to classify the accessions into morphotypes, i.e. groups of morphologically identical accessions. However, their genetic uniformity is currently unknown. The ISSR technique was used in two experiments to determine the relationships between both morphological and molecular markers systems. The intra-morphotype genetic diversity, the spatial structures of the diversity and the congruence between both markers systems were determined. In the first experience, 44 accessions representing five morphotypes, clearly distinct from each other, were analyzed. At the molecular level, the accessions exactly clustered according to their morphotypes. However, a genetic variability was observed inside each morphotype. In the second experiment, 34 accessions gradually differing from each other on morphological base were analyzed. The morphological clustering showed no geographical structure. On the opposite, the molecular analysis showed that the genetic structure was slightly related to the collection site. The correlation between both markers systems was weak but significant. The lack of perfect congruence between morphological and molecular data suggests that the morphological system may be useful for the morphotypes management but is not appropriate to study the genetic structure of the oca. The spatial structure of the genetic diversity can be related to the evolution of the species and the discordance between the morphological and molecular structures may result from similar selection pressures at different places leading to similar forms with a different genetic background.

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Acknowledgements

This research has been supported by a FRIA (Fonds pour la formation à la Recherche dans l’Industrie et dans l’Agriculture, Belgium) fellowship to the first author. The authors thank the FNRS (Fonds National de la Recherche Scientifique, Belgium) for providing financial support for this work (FRFC 2.4507.03). Thanks also to the INIEA (Instituto Nacional de Investigación y Extensión Agraria, Peru) to have delivered appropriate authorization to study the oca germplasm.

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Authors and Affiliations

  1. Département de Biologie Appliquée et Productions Agricoles, Ecophysiologie et Amélioration Végétale, Université Catholique de Louvain, Place Croix du Sud, 2 bte 11, 1348, Louvain-La-Neuve, Belgium

    Audrey Pissard, Anne-Michèle Faux, Sébastien Paulet & Pierre Bertin

  2. International Potato Center (CIP), Apartado 1558, Lima 12, Peru

    Carlos Arbizu & Marc Ghislain

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  1. Audrey Pissard
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  2. Carlos Arbizu
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  3. Marc Ghislain
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  4. Anne-Michèle Faux
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  5. Sébastien Paulet
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  6. Pierre Bertin
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Correspondence to Audrey Pissard.

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Pissard, A., Arbizu, C., Ghislain, M. et al. Congruence between morphological and molecular markers inferred from the analysis of the intra-morphotype genetic diversity and the spatial structure of Oxalis tuberosa Mol.. Genetica 132, 71–85 (2008). https://doi.org/10.1007/s10709-007-9150-9

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  • DOI: https://doi.org/10.1007/s10709-007-9150-9

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