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A tandemly repetitive centromeric DNA sequence of the fish Hoplias malabaricus (Characiformes: Erythrinidae) is derived from 5S rDNA

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Abstract

A substantial fraction of the eukaryotic genome consists of repetitive DNA sequences that include satellites, minisatellites, microsatellites, and transposable elements. Although extensively studied for the past three decades, the molecular forces that generate, propagate and maintain repetitive DNAs in the genomes are still discussed. To further understand the dynamics and the mechanisms of evolution of repetitive DNAs in vertebrate genome, we searched for repetitive sequences in the genome of the fish species Hoplias malabaricus. A satellite sequence, named 5SHindIII-DNA, which has a conspicuous similarity with 5S rRNA genes and spacers was identified. FISH experiments showed that the 5S rRNA bona fide gene repeats were clustered in the interstitial position of two chromosome pairs of H. malabaricus, while the satellite 5SHindIII-DNA sequences were clustered in the centromeric position in nine chromosome pairs of the species. The presence of the 5SHindIII-DNA sequences in the centromeres of several chromosomes indicates that this satellite family probably escaped from the selective pressure that maintains the structure and organization of the 5S rDNA repeats and become disperse into the genome. Although it is not feasible to explain how this sequence has been maintained in the centromeric regions, it is possible to hypothesize that it may be involved in some structural or functional role of the centromere organization.

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References

  • S.F. Altschul W. Gish W. Miller E.W. Myers D.J. Lipman (1990) ArticleTitleBasic local alignment search tool J. Mol. Biol. 215 403–410 Occurrence Handle2231712 Occurrence Handle1:CAS:528:DyaK3MXitVGmsA%3D%3D Occurrence Handle10.1006/jmbi.1990.9999

    Article  PubMed  CAS  Google Scholar 

  • R. Beridze (1986) Satellite DNA Springer Berlin, Heidelberg, New York

    Google Scholar 

  • L.A.C. Bertollo C.A. Mestriner (1998) ArticleTitleThe X1X2Y sex chromosome system in the fish Hoplias malabaricus. II. Meiotic analyses Chromosome Res. 6 141–147 Occurrence Handle9543017 Occurrence Handle1:CAS:528:DyaK1cXitVWnsrk%3D Occurrence Handle10.1023/A:1009243114124

    Article  PubMed  CAS  Google Scholar 

  • L.A.C. Bertollo C.S. Takahashi O. Moreira-Filho (1978) ArticleTitleKaryotypic studies of two allopatric populations of the genus Hoplias (Pisces, Erythrinidae) Brazil. J. Genet. 2 17–37

    Google Scholar 

  • L.A.C. Bertollo C.S. Takahashi O. Moreira-Filho (1983) ArticleTitleMultiple sex chromosomes in the genus Hoplias (Pisces, Erythrinidae) Cytologia 48 1–12

    Google Scholar 

  • L.A.C. Bertollo O. Moreira-Filho M.S. Fontes (1997a) ArticleTitleKaryotypic diversity and distribution in Hoplias malabaricus (Pisces, Erythrinidae): Cytotypes with 2n. 40 chromosomes Brazil. J. Genet. 20 237–242

    Google Scholar 

  • L.A.C. Bertollo M.S. Fontes A.S. Fenocchio J. Cano (1997b) ArticleTitleThe X1X2Y sex chromosome system in the fish Hoplias malabaricus. I. G-, C- and chromosome replication banding Chromosome Res. 5 493–499 Occurrence Handle1:CAS:528:DyaK2sXnvVGrt7w%3D Occurrence Handle10.1023/A:1018477232354

    Article  CAS  Google Scholar 

  • L.A.C. Bertollo G.G. Born J.A. Dergam A.S. Fenocchio O. Moreira-Filho (2000) ArticleTitleA biodiversity approach in the Neotropical fish Hoplias malabaricus. Karyotypic survey, geographic distribution of cytotypes and cytotaxonomic considerations Chrom. Res. 8 603–613 Occurrence Handle11117356 Occurrence Handle1:CAS:528:DC%2BD3cXovVyku7g%3D Occurrence Handle10.1023/A:1009233907558

    Article  PubMed  CAS  Google Scholar 

  • G.G. Born L.A.C. Bertollo (2000) ArticleTitleAn XX/XY sex chromosome system in a fish species, Hoplias malabaricus, with a polymorphic NOR-bearing X chromosome Chromosome Res. 8 111–118 Occurrence Handle10780699 Occurrence Handle1:CAS:528:DC%2BD3cXislSnt74%3D Occurrence Handle10.1023/A:1009238402051

    Article  PubMed  CAS  Google Scholar 

  • S. Brenner G. Elgar R. Sandford A. Macrae B. Venkatesh S. Aparicio (1993) ArticleTitleCharacterization of the pufferfish (Fugu) genome as a compact model vertebrate genome Nature 6452 265–268 Occurrence Handle10.1038/366265a0

    Article  Google Scholar 

  • A. Canapa P.N. Cerioni M. Barucca E. Olmo V. Caputo (2002) ArticleTitleA centromeric satellite DNA may be involved in heterochromatin compactness in gobiid fishes Chrom. Res. 10 297–304 Occurrence Handle12199143 Occurrence Handle1:CAS:528:DC%2BD38XlsFKgsrc%3D Occurrence Handle10.1023/A:1016519708187

    Article  PubMed  CAS  Google Scholar 

  • T. Cavalier-Smith (1985) The Evolution of Genome Size Wiley New York

    Google Scholar 

  • B. Charlesworth P. Snlegowski W. Stephan (1994) ArticleTitleThe evolutionary dynamics of repetitive DNA in eukaryotes Nature 371 215–220 Occurrence Handle8078581 Occurrence Handle1:CAS:528:DyaK2cXmt1emu7o%3D Occurrence Handle10.1038/371215a0

    Article  PubMed  CAS  Google Scholar 

  • G. Childs R. Maxson R.H. Cohn L. Kedes (1981) ArticleTitleOrphons: dispersed genetic elements from tandem repetitive genes of eukaryotes Cell 23 651–663 Occurrence Handle6784929 Occurrence Handle1:CAS:528:DyaL3MXhs12ltLY%3D Occurrence Handle10.1016/0092-8674(81)90428-1

    Article  PubMed  CAS  Google Scholar 

  • C. Clabby U. Goswami F. Flavin N.P. Wilkins J.A. Houghton R. Powell (1996) ArticleTitleCloning, characterization and chromosomal location of a satellite DNA from the Pacific oyster, Crassostrea gigas Gene 168 205–209 Occurrence Handle8654945 Occurrence Handle1:CAS:528:DyaK28Xhslehurs%3D Occurrence Handle10.1016/0378-1119(95)00749-0

    Article  PubMed  CAS  Google Scholar 

  • F. Cremisi R. Vignali R. Batistoni G. Barsacchi (1988) ArticleTitleHeterochromatic DNA in Triturus (Anphibia, Urodela) II. A centromeric satellite DNA Chromosoma 97 204–211 Occurrence Handle3219917 Occurrence Handle1:CAS:528:DyaL1MXhtV2htb0%3D Occurrence Handle10.1007/BF00292962

    Article  PubMed  CAS  Google Scholar 

  • E.M. Denovam J.M. Wright (1990) ArticleTitleA satellite DNA family from pollock (Pollachius virens) Gene 87 279–283 Occurrence Handle10.1016/0378-1119(90)90313-G

    Article  Google Scholar 

  • J.A. Dergam L.A.C. Bertollo (1990) ArticleTitleKaryotypic diversi¢cation in Hoplias malabaricus (Osteichthyes, Erythrinidae) of the São Francisco and Alto Paraná basins, Brazil Brazil. J. Genet. 13 755–766

    Google Scholar 

  • G.A. Dover (1986) ArticleTitleLinkage disequilibrium and molecular drive in the rDNA gene family Genetics 122 249–252

    Google Scholar 

  • G. Drouin M. Moniz Sá Particlede (1995) ArticleTitleThe concerted evolution of 5S ribosomal genes linked to the repeat units of other multigene families Mol. Biol. Evol. 12 481–493 Occurrence Handle7739390 Occurrence Handle1:CAS:528:DyaK2MXltF2nur4%3D

    PubMed  CAS  Google Scholar 

  • E.E. Eichler D. Sankoff (2003) ArticleTitleStructural Dynamics of eukaryotes chromosome evolution Science 301 793–797 Occurrence Handle12907789 Occurrence Handle1:CAS:528:DC%2BD3sXmtVGqsLg%3D Occurrence Handle10.1126/science.1086132

    Article  PubMed  CAS  Google Scholar 

  • B.M. Emerson R.G. Roeder (1984) ArticleTitleIsolation and genomic arrangement of active and inactive forms of mammalian 5S RNA genes J. Biol. Chem. 259 7916–7925 Occurrence Handle6203899 Occurrence Handle1:CAS:528:DyaL2cXksV2nu7s%3D

    PubMed  CAS  Google Scholar 

  • J. Falquet R. Creusot M. Dron (1997) ArticleTitleMolecular analysis of DNA homologous to IGS subrepeats Plant Physiol. Biochem. 35 611–622 Occurrence Handle1:CAS:528:DyaK2sXlslejt78%3D

    CAS  Google Scholar 

  • J. Felsenstein (1985) ArticleTitleConfidence limits on phylogenies: An approach using the bootstrap Evolution 39 783–791 Occurrence Handle10.2307/2408678

    Article  Google Scholar 

  • M.A. Garrido-Ramos M. Jamilena R. Lozano C. Ruiz Rejón M. Ruiz Rejón (1995) ArticleTitleThe EcoRI centromeric satellite DNA of the Sparidae family (Pisces, Perciformes) contains a sequence motive common to other vertebrates centromeric satellite DNAs Cytogen. Cell Genet. 71 345–351 Occurrence Handle1:CAS:528:DyaK28Xot1egsA%3D%3D

    CAS  Google Scholar 

  • T. Haaf M. Schmid C. Steinlein P.M. Galetti H. Willard (1993) ArticleTitleOrganization and molecular cytogenetics of a satellite DNA family from Hoplias malabaricus (Pisces, Erythrinidae) Chromosome Res. 1 77–86 Occurrence Handle8143092 Occurrence Handle1:CAS:528:DyaK2MXmtV2muw%3D%3D Occurrence Handle10.1007/BF00710610

    Article  PubMed  CAS  Google Scholar 

  • S. Henikoff K. Ahmad H.S. Malik (2001) ArticleTitleThe centromere paradox: stable inheritance with rapidly evolving DNA Science 293 1098–1102 Occurrence Handle11498581 Occurrence Handle1:CAS:528:DC%2BD3MXmtVWku7o%3D Occurrence Handle10.1126/science.1062939

    Article  PubMed  CAS  Google Scholar 

  • J.E. Horvath J.A. Bailey D.P. Locke E.E. Eichler (2001) ArticleTitleLessons from the human genome: transitions between euchromatin and heterochromatin Hum. Mol. Genet. 10 2215–2223 Occurrence Handle11673404 Occurrence Handle1:CAS:528:DC%2BD3MXotVKhs7w%3D Occurrence Handle10.1093/hmg/10.20.2215

    Article  PubMed  CAS  Google Scholar 

  • M. Kato (1999) ArticleTitleStructural bistability of repetitive DNA elements featuring CA/TG dinucleotide steps and mode of evolution of satellite DNA Eur. J. Biochem. 265 204–209 Occurrence Handle10491175 Occurrence Handle1:CAS:528:DyaK1MXmsFKks78%3D Occurrence Handle10.1046/j.1432-1327.1999.00714.x

    Article  PubMed  CAS  Google Scholar 

  • H. Komiya S. Takemura (1979) ArticleTitleNucleotide sequence of 5S ribosomal RNA from rainbow trout (Salmo gairdnerii) liver J. Biochem. 86 1067–1080 Occurrence Handle115850 Occurrence Handle1:STN:280:DyaL3c%2FkvFGqug%3D%3D

    PubMed  CAS  Google Scholar 

  • S. Kumar K. Tamura I. Jakobsen M. Nei (2001) ArticleTitleMEGA: Molecular evolutionary genetic analysis Bioinformatics 12 1244–1249 Occurrence Handle10.1093/bioinformatics/17.12.1244

    Article  Google Scholar 

  • Z. Larin M.D. Fricker C. Tyler-Smith (1994) ArticleTitle De novo formation of several features of a centromere following introduction of a Y alphoid YAC into mammalian cells Hum. Mol. Genet. 3 689–695 Occurrence Handle8081354 Occurrence Handle1:CAS:528:DyaK2cXkt1yisro%3D

    PubMed  CAS  Google Scholar 

  • R. Leah S. Frederiksen J. Engberg P.D. Sorensen (1990) ArticleTitleNucleotide sequence of a mouse 5S rRNA variant gene Nucl. Acids Res. 18 7441–7441 Occurrence Handle2259634 Occurrence Handle1:CAS:528:DyaK3MXhsFyhtL4%3D

    PubMed  CAS  Google Scholar 

  • A.R. Lohe P.A. Roberts (1990) ArticleTitleAn unusual Y chromosome of Drosophila simulans carrying amplified rDNA spacer without RNA genes Genetics 125 399–406 Occurrence Handle2379820 Occurrence Handle1:CAS:528:DyaK3cXlsVKnsbs%3D

    PubMed  CAS  Google Scholar 

  • P.A. Lopes A.J. Alberdi J.A. Dergam A.S. Fenocchio (1998) ArticleTitleCytotaxonomy of Hoplias malabaricus (Osteichthyes, Erythrinidae) in the Aguapey river (Province of Corrientes, Argentina) Copeia 1998 485–487 Occurrence Handle10.2307/1447447

    Article  Google Scholar 

  • S. Ohno U. Wolf N.B. Atkin (1968) ArticleTitleEvolution from fish to mammals by gene duplication Hereditas 59 169–187 Occurrence Handle5662632 Occurrence Handle1:STN:280:DyaF1czivVynsw%3D%3D Occurrence Handle10.1111/j.1601-5223.1968.tb02169.x

    Article  PubMed  CAS  Google Scholar 

  • D. Pinkel T. Straume J. Gray (1986) ArticleTitleCytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization Proc. Natl. Acad. Sci. 83 2934–2938 Occurrence Handle3458254 Occurrence Handle1:CAS:528:DyaL28XktlynsbY%3D Occurrence Handle10.1073/pnas.83.9.2934

    Article  PubMed  CAS  Google Scholar 

  • G. Pont F. DeGroote G. Picard (1987) ArticleTitleSome extrachromosomal circular DNAs from Drosophila embryos are homologous to tandemly repeated genes J. Mol. Biol. 195 447–451 Occurrence Handle3116263 Occurrence Handle1:CAS:528:DyaL2sXksFehu74%3D Occurrence Handle10.1016/0022-2836(87)90665-6

    Article  PubMed  CAS  Google Scholar 

  • S. Renault F. DeGroote G. Picard (1993) ArticleTitleIdentification of short tandemly repeated sequences in extrachromosomal circular DNAs from Drosophila melanogaster embryos Genome 36 244–254 Occurrence Handle8514153 Occurrence Handle1:CAS:528:DyaK3sXmsVagtLw%3D

    PubMed  CAS  Google Scholar 

  • J. Sambrook D.W. Russel (2001) Molecular Cloning. A Laboratory Manual Third Edition Cold Spring Harbor Laboratory Press Cold Spring Harbor, New York

    Google Scholar 

  • D. Sart M.R. Cancilla E. Earle J.I. Mao R. Saffery K.M. Tainton P. Kalitsis J. Martyn A.E. Barry K.H. Choo (1997) ArticleTitleA functional neo-centromere formed through activation of a latent human centromere and consisting of non-alpha-satellite DNA Nature Genet. 16 144–153 Occurrence Handle9171825 Occurrence Handle10.1038/ng0697-144

    Article  PubMed  Google Scholar 

  • M.F. Singer (1982) ArticleTitleHighly repetitive sequences in mammalian genomes Int. Rev. Cytol. 76 67–112 Occurrence Handle6749748 Occurrence Handle1:CAS:528:DyaL38XlslGqurs%3D

    PubMed  CAS  Google Scholar 

  • E.M. Southern (1975) ArticleTitleDetection of specific sequences among DNA fragments separated by gel electrophoresis J. Mol. Biol. 98 503–517 Occurrence Handle1195397 Occurrence Handle1:CAS:528:DyaE28XktVeltQ%3D%3D

    PubMed  CAS  Google Scholar 

  • A.T. Sumner (1972) ArticleTitleA simple technique for demonstrating centromeric heterochromatin Exp. Cell Res. 75 304–306 Occurrence Handle4117921 Occurrence Handle1:STN:280:DyaE3s%2FkslGitA%3D%3D Occurrence Handle10.1016/0014-4827(72)90558-7

    Article  PubMed  CAS  Google Scholar 

  • J.D. Thompson D.G. Higgins T.J. Gibson (1994) ArticleTitleClustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice Nucl. Acids Res. 22 4673–4680 Occurrence Handle7984417 Occurrence Handle1:CAS:528:DyaK2MXitlSgu74%3D

    PubMed  CAS  Google Scholar 

  • A. Viñas M. Abuín B.G. Pardo P. Martínez L. Sánchez (2004) ArticleTitleCharacterization of a new HpaI centromeric satellite DNA in Salmo salar Genetica 121 81–87 Occurrence Handle15098740 Occurrence Handle10.1023/B:GENE.0000019927.30049.9c

    Article  PubMed  Google Scholar 

  • B. Vissel A. Nagy K.H.A. Choo (1992) ArticleTitleA satellite III sequence shared by human chromosomes 13, 14 and 21 that is contiguous with alpha satellite DNA Cytogen. Cell Genet. 61 81–86 Occurrence Handle1:CAS:528:DyaK3sXhvVCkurY%3D Occurrence Handle10.1159/000133374

    Article  CAS  Google Scholar 

  • A.K.C. Wong J.B. Rattner (1988) ArticleTitleSequence organization and cytological localization of the minor satellite of mouse Nucl. Acids Res. 16 11645–11661 Occurrence Handle3211746 Occurrence Handle1:CAS:528:DyaL1MXlt1OqsLk%3D

    PubMed  CAS  Google Scholar 

  • J.M. Wright (1989) ArticleTitleNucleotide sequence, genomic organization and the evolution of a major repetitive DNA family in tilapia (Oreochromis mossambicus/hornorum) Nucl. Acids Res. 17 5071–5079 Occurrence Handle2762120 Occurrence Handle1:CAS:528:DyaL1MXlt1yjsbs%3D

    PubMed  CAS  Google Scholar 

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  1. Departamento de Morfologia, UNESP-Universidade Estadual Paulista, Instituto de Biociências, CEP 18618-000, Botucatu, SP, Brazil

    Cesar Martins, Irani Alves Ferreira, Claudio Oliveira & Fausto Foresti

  2. Departamento de Genética e Evolução, Universidade Federal de São Carlos, Centro de Ciências Biológicas e da Saúde, CEP 13565-905, São Carlos, SP, Brazil

    Pedro Manoel Galetti Jr

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  1. Cesar Martins
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  2. Irani Alves Ferreira
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  3. Claudio Oliveira
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  4. Fausto Foresti
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  5. Pedro Manoel Galetti Jr
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Correspondence to Cesar Martins.

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Martins, C., Ferreira, I.A., Oliveira, C. et al. A tandemly repetitive centromeric DNA sequence of the fish Hoplias malabaricus (Characiformes: Erythrinidae) is derived from 5S rDNA. Genetica 127, 133–141 (2006). https://doi.org/10.1007/s10709-005-2674-y

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