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The effects of Roundup on gametes and early development of common carp (Cyprinus carpio L)

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Abstract

To determine the effects of Roundup, a commercial formulation of glyphosate, gametes, and embryos of common carp (Cyprinus carpio L) was exposed to wide range of herbicide concentrations (0.0, 0.1, 0.5, 2.0, 5.0, 10.0, 20.0, and 50.0 mg/l). The obtained results showed different effects of Roundup on common carp gametes. Herbicide reduced swelling of eggs (but the effect was not concentration-related), while sperm showed low sensitivity to Roundup (time of spermatozoa motility was reduced in a significant way only at 20 mg/l, and at remaining concentrations, only a slight tendency was observed). During the embryonic development, Roundup caused a decrease of common carp embryonic survival (and the effect was concentration-related); however, it had no effect on development rate. During the embryogenesis, three types of embryo body malformation were observed: yolk sac edema, spine curvature, and shortening of body, but their frequencies were not associated with the presence or concentration of herbicide. However, Roundup affected quality of newly hatched larvae of common carp by increasing their mortality. No effect of herbicide on percentage of deformed larvae was observed but larvae hatched in water with Roundup tended to show more complex anomalies compared to those from the control. Obtained data showed that even low concentrations of this herbicide in waters can significantly reduce egg swelling, survival of embryos, and quality of fish larvae.

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References

  • Acquavella JF, Bruce H, Alexander BH, Mandel JS, Gustin C, Baker B, Chapman P, Bleeke M (2004) Glyphosate biomonitoring for farmers and their families: results from the farm family exposure study. Environ Health Perspect 112:321–326

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Alonzo HGA, Correa CL (2008) In: Oga, S., Camargo, M.M.A., Batistuzzo, J.A.O. (Eds.), Praguicidas. 3aed. Atheneu Editora, São Paulo, pp. 621–642

  • Ayoola SO (2008) Toxicity of glyphosate herbicide on Nile tilapia (Oreochromis niloticus) juvenile. Afr J Agric Res 3(12):825–834

    Google Scholar 

  • Baylis AD (2000) Why glyphosate is a global herbicide: strengths, weaknesses and prospects. Pest Manag Sci 56:299–308

    Article  CAS  Google Scholar 

  • Busse MD, Ratcliff AW, Shestak CJ, Powers RF (2001) Glyphosate toxicity and effects of long-term vegetation control on soil microbial communities. Soil Biol Biochem 33:1777–1789

    Article  CAS  Google Scholar 

  • Cavalli VLLO, Cattani D, Heinz Rieg CE, Pierozan P, Zanatta L, Parissoto EB, Filho DW, Mena Barreto Silva FR, Pessoa-Pureur R, Zamoner A (2013) Roundup disrupts male reproductive functions by triggering calcium-mediatedcell death in rats testis and Sertoli cells. Free Radic Biol Med 65:335–346

    Article  CAS  Google Scholar 

  • Cavas T, Konen S (2007) Detection of cytogenetic and DNA damage in peripheral erythrocytes of goldfish (Carassius auratus) exposed to a glyphosate formulation using the micronucleus test and the comet assay. Mutagenesis 22(4):263–268

    Article  PubMed  CAS  Google Scholar 

  • Clair A, Mesnage R, Travert C, Seralini GEA (2012) Glyphosatebased herbicide induces necrosis and apoptosis in mature rat testicularncells in vitro, and estosterone decrease at lower levels. Toxicol Vitro 26(2):269–279

    Article  CAS  Google Scholar 

  • Contardo-Jara V, Klingelmann E, Wiegant C (2008) Bioaccumulation glyphosate and its formulation Roundup in lumbriculus variegates and its effects on biotransformation and antioxidants enzymes. Environ Pollut 157:57–63

    Article  PubMed  CAS  Google Scholar 

  • Costa MJ, Monteiro DA, Oliveira-Neto AL, Rantin FT, Kalinin AL (2008) Oxidative stress biomarkers and heart function in bullfrog tadpoles exposed to Roundup Original®. Ecotoxicology 17(3):153–163

    Article  PubMed  CAS  Google Scholar 

  • Cox C, Surgan M (2006) Unidentified inert ingredients in pesticides: implications for human and environmental health. Environ Health Perspect 114:1803–1806

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Fan J, Geng J, Ren H, Wang X (2013) Time-effect relationship of toxicity induced by Roundup® and its main constituents in liver of Carassius auratus. Comput Water Energy Environ Eng 2:20–25

    Article  Google Scholar 

  • Folmar LC, Sanders HO, Julin AM (1979) Toxicity of the herbicide glyphosate and several of its formulations to fish and aquatic invertebrates. Arch Environ Contain Toxicol 8:269–278

    Article  CAS  Google Scholar 

  • Franz JE, Mao MK, Sikorski JA (1997) Glyphosate: a unique global herbicide. ACS monograph 189. Am Chem Soc, Washington, DC, pp. 653

  • Giesy JP, Dobson S, Solomon KR (2000) Ecotoxicological risk assessment for Roundups herbicide. Rev Environ Contam Toxicol 167:35–120

    CAS  Google Scholar 

  • Gimsing AL, Borggaard OK, Bang M (2004) Influence of soil composition on adsorption of glyphosate and phosphate by contrasting Danish surface soils. Eur J Soil Sci 55:183–191

    Article  CAS  Google Scholar 

  • Glusczak L, Miron DDS, Crestani M, da Fonseca MB, Pedron FDA, Duarte MF, Vieira VUP (2006) Effect of glyphosate herbicide on acetylcholinesterase activity and metabolic and hematological parameters in piava (Leporinusobtusidens). Ecotoxicol Environ Saf 65:237–241

    Article  PubMed  CAS  Google Scholar 

  • Glusczak L, Miron DDS, Moraes BS, Simoes RR, Schetinger MRC, Morsch VM, Loro VL (2007) Acute effects of glyphosate herbicide on metabolic and enzymatic parameters of silver catfish (Rhamdia quelen). Comp Biochem Physiol 146C:519–524

    CAS  Google Scholar 

  • Guilherme S, Santos MA, Barroso C, Gaivao I, Pacheco M (2012) Differential genotoxicity of Roundup formulation and its constituents in blood cells of fish (Anguilla Anguilla): considerations on chemical interactions and DNA damaging mechanisms. Ecotoxicology 21(5):1381–1390

    Article  PubMed  CAS  Google Scholar 

  • Howe CM, Berrill M, Pauli BD, Helbing CC, Werry K, Veldhoen N (2004) Toxicity of glyphosate-based pesticides to four North American frog species. Environ Toxicol Chem 23:1928–1938

    Article  PubMed  CAS  Google Scholar 

  • Jezierska B, Bartnicka B (1995) The effect of pH on embryonic development of carp (Cyprinus carpio L.). Aquaculture 129(1–4):133–134

    Article  Google Scholar 

  • Jezierska B, Lugowska K, Witeska M, Sarnowski P (2000) Malformations of newly hatched common carp larvae. Electronic Journal of Polish Aquacultural Universities. Fisheries 3 (2)

  • Jezierska B, Lugowska K, Witeska M (2009) The effects of heavy metals on embryonic development of fish (a review). Fish Physiol Biochem 35:625–640

    Article  PubMed  CAS  Google Scholar 

  • Jiraungkoorskul W, Upatham ES, Kruatrachue M, Sahaphong S, Vichasri-Grams S, Pokethitiyook P (2002) Histopathological effects of Roundup, a glyphosate herbicide, on Nile tilapia (Oreochromisniloticus). Sci Asia 28:121–127

    Article  CAS  Google Scholar 

  • Jofre DM, Germano García MJ, Salcedo R, Morales M, Alvarez M, Enriz D, Giannini F (2013) Fish toxicity of commercial herbicides formulated with glyphosate. J Environ Anal Toxicol:4–1

  • Kelly DW, Poulin R, Tompkins DM, Townsend CR (2014) Synergistic effects of glyphosate formulation and parasite infection on fish malformations and survival. J Appl Ecol 47:498–504

    Article  CAS  Google Scholar 

  • Kreutz LC, Gil Barcellos LJ, de Faria Valle S, de Oliveira Silva T, Anziliero D, Davi dos Santos E, Pivato M, Zanatta R (2011) Altered hematological and immunological parameters in silver catfish Rhamdia quelen following short term exposure to sublethal concentration of glyphosate. Fish Shellfish Immunol 30(1):51–57

    Article  PubMed  CAS  Google Scholar 

  • Lopes FM, Varela Junior AS, Corcini CD, da Silva AC, Guazzelli VG, Tavares G, da Rosa CE (2014) Effect of glyphosate on the sperm quality of zebrafish Danio rerio. Aquat Toxicol 155:322–326

    Article  PubMed  CAS  Google Scholar 

  • Lugowska K (2009) Embryonic development of barbel (Barbus barbus). Isr J Aquacult Bamidgeh 61:68–72

    Google Scholar 

  • Modesto KA, Martinez CBR (2010) Effects of Roundup Transorb on fish: hemato-logy, antioxidant defenses and acetylcholinesterase activity. Chemosphere 81:781–787

    Article  PubMed  CAS  Google Scholar 

  • Monheit S (2007) Glyphosate-based aquatic herbicides. An overview of risk. [Online]. Available: http://teamarundo.org/control manage/docs/glyphosate aqua risk. pdf

  • Neskovic NK, Poleksic V, Elezovic I, Karan V, Budimir M (1996) Biochemical and histopathological effects of glyphosate on carp, Cyprinus carpio L. Bull Environ Contam Toxicol 56:295–302

    Article  PubMed  CAS  Google Scholar 

  • Nwani CD, Ibiam UA, Ibiam OU, Nworie O, Onyishi G, Atama C (2013) Investigation on acute toxicity and behavioral changes in Tilapia zilli due to glyphosate-based herbicide, forceup J. Anim. Plant Sci 23 (3)

  • Peixoto F (2005) Comparative effects of the Roundup and glyphosate on mitochondrial oxidative phosphorylation. Chemosphere 61:1115–1122

    Article  PubMed  CAS  Google Scholar 

  • Ramírez-Duarte WF, Rondon-Barragan LS, Eslava-Mocha PR (2008) Acute toxicity and histopathological alterations of Roundup® herbicide on “cachama blanca” (Piaractus brachypomus). Pesq Vet Bras 28(11):547–554

    Article  Google Scholar 

  • Relyea RA (2004) Growth and survival of five amphibian species exposed to combinations of pesticides. Environ Toxicol Chem 23:1737–1742

    Article  PubMed  CAS  Google Scholar 

  • Relyea RA (2005) The impact of insecticides and herbicides on the biodiversity and productivity of aquatic communities. Ecol Appl 15:618–627

    Article  Google Scholar 

  • Romano MA, Romano RM, Santos LD, Wisniewski P, Campos DA, Souza PB, Vlau P, Bernardi MM, Nunes MT, Oliveira CA (2012) Glyphosate impairs male offspring reproductive development by disrupting gonadotropin expression. Reprod Toxicol 86:663–673

    CAS  Google Scholar 

  • Rurangwa E, Kime DE, Ollevier F, Nash JP (2004) The measurement of sperm motility and factors affecting sperm quality in cultured fish. Aquaculture 234:1–28

    Article  Google Scholar 

  • Sancho E, Cerón JJ, Ferrando MD (2000) Cholinesterase activity and hematological parameters as biomarkers of sublethal molinate exposure in Anguilla anguilla. Ecotoxicol Environ Saf 46:81–86

    Article  PubMed  CAS  Google Scholar 

  • Slominska I (1998) Sensitivity of early developmental stages of common carp (Cyprinus carpio L.) to lead and copper toxicity. Ph. D thesis, Institute of inland fisheries, Olsztyn, pp. 104 (In Polish)

  • Sulukan E, Kokturk M, Ceylan H, Beydemir S, Isik M, Atamanalp M, Ceyhun SB (2017) An approach to clarify the effect mechanism of glyphosate on body malformations during embryonic development of zebrafish (Danio rerio). Chemosphere 180:77–85

    Article  PubMed  CAS  Google Scholar 

  • Szarek J, Siwicki A, Andrzejewska A, Terech-Majewska E, Banaszkiewicz T (2000) Effects of the herbicide RoundupTM on the ultrastructural pattern of hepatocytes in carp (Cyprinus carpio). Mar Environ Res 50:263–266

    Article  PubMed  CAS  Google Scholar 

  • Tsui MTK, Chu LM (2003) Aquatic toxicity of glyphosate-based formulations: comparison between different organisms and the effects of environmental factors. Chemosphere 52:1189–1197

    Article  PubMed  CAS  Google Scholar 

  • Uren-Webster TM, Laing LV, Florence H, Santos EM (2014) Effects of glyphosate and its formulation, Roundup, on reproduction in zebrafish (Danio rerio). Environ Sci Technol 48:1271–1279

    Article  PubMed  CAS  Google Scholar 

  • Velasques RR, Zomer Sandrini J, da Rosa CE (2016) Roundup in zebrafish: effects on oxidative status and gene expression. Zebrafish 00:1–10

    Google Scholar 

  • Walsh LP, McCormick C, Marin C, Stocco DM (2000) Roundup inhibits steroidogenesis by disrupting steroidogenic acute regulatory (StAR) protein expression. Environ Health Perspect 108:769–776

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • WHO (1994) Glyphosate. In: Environmental Health Criteria, no. 159, World Health Organization, Geneva, Switzerland, ISBN 92-4-157159-4:177

  • Witeska M, Jezierska B, Chaber J (1995) The influence of cadmium on common carp embryos and larvae. Aquaculture 129:129–132

    Article  CAS  Google Scholar 

  • Yousof S, Ahmad I, Mohamad SA (2014) Effect of glyphosate-based herbicide on early life stages of java medaka (Oryzias javanicus): a potential tropical test fish. Marine Pollution Bull 85:494–498

    Article  CAS  Google Scholar 

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Funding

The present study was financed from funds provided by the Siedlce University of Natural Science and Humanities, project No 387/14/S.

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  1. Department of Animal Physiology, Siedlce University of Natural Sciences and Humanities, Prusa 12, 08-110, Siedlce, Poland

    Katarzyna Lugowska

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  1. Katarzyna Lugowska
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Correspondence to Katarzyna Lugowska.

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Lugowska, K. The effects of Roundup on gametes and early development of common carp (Cyprinus carpio L). Fish Physiol Biochem 44, 1109–1117 (2018). https://doi.org/10.1007/s10695-018-0498-9

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  • DOI: https://doi.org/10.1007/s10695-018-0498-9

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