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Effects of Rhizoma Alismatis extract on biochemical indices and adipose gene expression in oleic acid-induced hepatocyte injury in Jian carp (Cyprinus carpio var. Jian)

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Abstract

Fatty liver is an increasingly serious disease of fish in aquaculture. However, the mechanisms responsible for the occurrence of fatty liver remain unclear, and no effective methods for the prevention and treatment of this disease have yet been found. In the present study, we aimed to develop an in vitro model of hepatocyte injury using oleic acid as hepatotoxicant and evaluate the protective effects of Rhizoma Alismatis extract (RAE) in Jian carp using this model. Primary hepatocytes from Jian carp were isolated and purified and cultured in vitro. The result indicated that 0.4 mmol L−1 oleic acid and 48 h could be the optimal conditions to induce hepatocyte injury model in cultured hepatocytes. Hepatocytes were exposed to oleic acid, followed by the addition of RAE at 0, 1, 5, 10, 20, or 50 μg mL−1. The hepatocytes and supernatant were then analyzed. RAE suppressed oleic acid-induced elevations in aspartate aminotransferase, alanine aminotransferase, triglycerides, total cholesterol, lactate dehydrogenase, alkaline phosphatase, cholinesterase, malondialdehyde, γ-glutamyl transferase, cytochrome P450 1A, cytochrome P450 2E1, liver-type fatty acid binding protein, free fatty acid, fatty acid synthetase, and tumor necrosis factor-α (P < 0.01 or P < 0.05); reduced protein levels of cytochrome P450 1A, nuclear factor (NF)-κB p65, and NF-κB c-Rel; and inhibited cytochrome P4503A, NF-κB c-Rel, nuclear factor erythroid-related factor 2, peroxisome proliferator-activated receptor-α, and cytochrome P4501A mRNA levels. In conclusion, RAE exhibited a protective effect against hepatocyte injury in Jian carp. Further in vivo studies are needed to provide more evidence for the use of RAE as a hepatoprotective agent for the treatment of hepatocyte injury.

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Abbreviations

TC:

Total cholesterol

ROS:

Reactive oxygen species

TG:

Triglycerides

RAE:

Rhizoma Alismatis extract

FFA:

Free fatty acid

CHE:

Cholinesterase

GPT:

Glutamate pyruvate transaminase

ALP:

Alkaline phosphatase

GOT:

Glutamate oxalate transaminase

γ-GT:

γ-Glutamyl transferase

LDH:

Lactate dehydrogenase

CYP 1A:

Cytochrome P450 1A

MDA:

Malondialdehyde

CYP 3A:

Cytochrome P450 3A

PBS:

Phosphate-buffered saline

CYP2E1:

Cytochrome P4502E1

Nrf2:

Nuclear factor erythroid-related factor 2

IgG:

Immunoglobulin G

PPAR-α:

Peroxisome proliferator-activated receptor-α

FFA:

Free fatty acids

L-FABP:

Liver-type fatty acid binding protein

FAS:

Fatty acid synthase

ACoAC:

Acetyl coenzyme A carboxylase

LPO:

Lipid peroxide

FCS:

Fetal calf serum

NF-κB p65:

Nuclear factor (NF)-κB p65

NF-κB c-Rel:

Nuclear factor (NF)-κB c-Rel

TNF-α:

Tumor necrosis factor-α

NAFLD:

Non-alcoholic fatty liver disease

BCA:

Bicinchonininc acid

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Acknowledgments

This work was supported by the Central Public-Interest Scientific Institution Basal Research Fund of China (2015JBFM01).

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Authors and Affiliations

  1. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China

    Jinliang Du, Rui Jia, Li-ping Cao, Weidong Ding, Pao Xu & Guojun Yin

  2. International Joint Research Laboratory for Fish Immunopharmacology, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China

    Jinliang Du, Rui Jia, Li-ping Cao, Weidong Ding, Pao Xu & Guojun Yin

  3. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China

    Pao Xu & Guojun Yin

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  1. Jinliang Du
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  2. Rui Jia
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  3. Li-ping Cao
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  4. Weidong Ding
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  5. Pao Xu
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  6. Guojun Yin
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Correspondence to Pao Xu or Guojun Yin.

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Du, J., Jia, R., Cao, Lp. et al. Effects of Rhizoma Alismatis extract on biochemical indices and adipose gene expression in oleic acid-induced hepatocyte injury in Jian carp (Cyprinus carpio var. Jian). Fish Physiol Biochem 44, 747–768 (2018). https://doi.org/10.1007/s10695-017-0428-2

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  • DOI: https://doi.org/10.1007/s10695-017-0428-2

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