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Interactive effects of dietary leucine and isoleucine on growth, blood parameters, and amino acid profile of Japanese flounder Paralichthys olivaceus

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Abstract

A 60-day feeding trial was conducted to assess the interactions of dietary leucine (Leu) and isoleucine (Ile) on Japanese flounder. Fish of 2.69 ± 0.04 g were fed experimental diets containing two levels of Leu (2.58 and 5.08% of diet) combined with three levels of Ile (1.44, 2.21, and 4.44% of diet), respectively. After the feeding trial, growth, proximate composition, muscle total amino acid profile, blood parameters, mucus lysozyme activity, and stress tolerance to freshwater were measured. Statistically significant (P < 0.05) interactive effects of Leu and Ile were found on growth parameters (final body weight, body weight gain, and special growth rate) of Japanese flounder. Antagonism was discovered in high dietary Leu groups, while stimulatory effects were obtained for increased dietary Ile in low Leu groups. Interactive effects of these two branched-chain amino acids were also found on hepatosomatic index of test fish. In addition, crude lipid content of fish whole body was significantly altered by various diets, with antagonism observed in low dietary Leu groups. Interactive effects also existed in muscle amino acid profiles for low fish meal diets, but no interactive impacts were observed on blood parameters. Furthermore, lysozyme activities and freshwater stress were significantly affected by different diets. And antagonism was found on lysozyme activities in low Leu groups. Moreover, high Leu and high Ile levels of diet significantly altered freshwater stress tolerance of Japanese flounder. These findings suggested that dietary Leu and Ile can effect interactively, and fish fed with diets containing 2.58% Leu with 4.44% Ile and 5.08% Leu with 1.44% Ile showed better growth performance.

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Acknowledgments

The first author would like to thank the corresponding author for his entire assistant during this research.

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Authors and Affiliations

  1. Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea, Ministry of Agriculture, Dalian Ocean University, 52 Heishijiao Street, Dalian, 116023, China

    Liping Wang, Yuzhe Han, Zhiqiang Jiang, Menglei Sun, Bin Si, Fei Chen & Ning Bao

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  1. Liping Wang
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  2. Yuzhe Han
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  3. Zhiqiang Jiang
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  4. Menglei Sun
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  7. Ning Bao
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Correspondence to Yuzhe Han.

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This research was funded by Grants from Public Science and Technology Research Funds Projects of Ocean of State Oceanic Administration People’s Republic of China (grant no. 201405003) and the Initial Project for Doctors of Liaoning Province (grant no. 201601289).

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Wang, L., Han, Y., Jiang, Z. et al. Interactive effects of dietary leucine and isoleucine on growth, blood parameters, and amino acid profile of Japanese flounder Paralichthys olivaceus . Fish Physiol Biochem 43, 1265–1278 (2017). https://doi.org/10.1007/s10695-017-0370-3

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