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Sublethal effects of pulp and paper mill effluent on two commonly cultured carps: a SEM- and EDS-based hematological biomarker analysis

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Abstract

Blood being a vehicle for the transport of industrial pollutants in living system, fish hematology is considered as potent biomarker. In the present study, we investigated respective sublethal effects of pulp and paper mill effluents on hematology of two commonly cultured carps, Cyprinus carpio and Ctenopharyngodon idella, using optical, scanning electron microscopy and energy-dispersive X-ray spectroscopy. Irrespective of species, results showed significant decrease in erythrocyte, hematocrit and hemoglobin contents while an increase in white blood cell counts (P < 0.05). We observed an increasing trend of MCV (170.0 ± 3.07 to 193.16 ± 2.5) and MCH (34.31 ± 1.89 to 38.71 ± 3.61) up to 28th day in C. carpio (P < 0.05), while, in C. idella, the highest percent increase in MCV (180.8 ± 2.19) and MCH (32.9 ± 0.62) was observed on seventh exposure day, which subsequently declined, respectively, to 173.1 ± 17.1 and 27.9 ± 2.45 on 28th day. Unlike C. carpio, significant and progressive MCHC declining trend (18.23 ± 0.28 to 16.13 ± 0.31) was observed in C. idella. The most commonly observed abnormalities under SEM include echinocytes, cytoplasmic blebbing, cytoplasmic ring, spherocytes, lobopodial projections and acanthocytes in red blood cells of exposed fishes. EDS further revealed the presence of aluminum, antimony, arsenic, cadmium, mercury, tungsten, zinc and titanium; some of these metals were not even detected in the effluent samples, suggesting the probable metal bio-concentration in fish tissue, and subsequent jeopardization is a major concern particularly in the industrial area. Our study further suggested the use of sensitive and specific techniques like SEM and EDS in fish hematological biomarker analysis along with the conventional approach.

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Acknowledgments

We acknowledge the support of SAIF, NEHU, Shillong, for providing electron microscopy facilities and Biotech Hub, Life Science, AUS, Silchar, for light microscopic facilities. This study complies with the necessary ethical standards.

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  1. Aquatic Toxicology and Remediation Laboratory, Department of Life Science and Bioinformatics, Assam University, Silchar, 788011, India

    Sangeeta Dey & Suchismita Das

  2. Ethnobotany and Medicinal Plants Research Laboratory, Department of Life Science and Bioinformatics, Assam University, Silchar, 788011, India

    Manabendra Dutta Choudhury

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  1. Sangeeta Dey
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Correspondence to Suchismita Das.

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Dey, S., Choudhury, M.D. & Das, S. Sublethal effects of pulp and paper mill effluent on two commonly cultured carps: a SEM- and EDS-based hematological biomarker analysis. Fish Physiol Biochem 42, 1791–1805 (2016). https://doi.org/10.1007/s10695-016-0258-7

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