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Purification of glucose-6-phosphate dehydrogenase and glutathione reductase enzymes from the gill tissue of Lake Van fish and analyzing the effects of some chalcone derivatives on enzyme activities

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An Erratum to this article was published on 03 March 2016

Abstract

Glucose-6-phosphate dehydrogenase (G6PD) and glutathione reductase (GR) are metabolically quite important enzymes. Within this study, these two enzymes were purified for the first time from the gills of Lake Van fish. In the purifying process, ammonium sulfate precipitation and 2′,5′-ADP Sepharose 4B affinity column chromatography techniques for glucose-6-phosphate dehydrogenase, temperature degradation and 2′,5′-ADP Sepharose 4B affinity column chromatography for glutathione reductase enzyme were used. The control of the enzyme purity and determination of molecular weight were done with sodium dodecyl sulfate polyacrylamide gel electrophoresis. K M and V max values were determined with Lineweaver–Burk plot. Besides, the effects of some chalcone derivatives on the purified enzymes were analyzed. For the ones showing inhibition effect, % activity–[I] figures were drawn and IC50 values were determined. K i value was calculated by using Cheng–Prusoff equation.

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Acknowledgments

This research was supported by the Ağrı İbrahim Çeçen University Unit of Scientific Research Projects, Project number FEF.12.004.

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Authors and Affiliations

  1. Faculty of Pharmacy, University of Ağrı İbrahim Çeçen, 04100, Ağrı, Turkey

    Muslum Kuzu & Naim Uzun

  2. Department of Nutrition and Dietetics, University of Ağrı İbrahim Çeçen, Ağrı, Turkey

    Abdulselam Aslan, Veysel Comakli & Ramazan Demirdag

  3. Karlsruhe Institute of Technology, Karlsruhe, Germany

    Ishtiaq Ahmed

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  1. Muslum Kuzu
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  2. Abdulselam Aslan
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  3. Ishtiaq Ahmed
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  4. Veysel Comakli
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  5. Ramazan Demirdag
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  6. Naim Uzun
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Correspondence to Muslum Kuzu.

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Kuzu, M., Aslan, A., Ahmed, I. et al. Purification of glucose-6-phosphate dehydrogenase and glutathione reductase enzymes from the gill tissue of Lake Van fish and analyzing the effects of some chalcone derivatives on enzyme activities. Fish Physiol Biochem 42, 483–491 (2016). https://doi.org/10.1007/s10695-015-0153-7

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  • DOI: https://doi.org/10.1007/s10695-015-0153-7

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