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Transferability of oxytetracycline (OTC) from feed to carp muscle and evaluation of the antibiotic effects on antioxidant systems in liver and kidney

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Abstract

Oxytetracycline (OTC) is employed in fish farms to contest or prevent bacterial infections. We simulated an OTC treatment at therapeutic level (75 mg kg−1) and at higher doses (150, 300 mg kg−1) for 10 days. A withdrawal period of 10 days was considered for treated carp, carrying out the same chemical and biochemical analyses (total glutathione, superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione S-transferase and malondialdehyde). The aim was to obtain data related to the carryover in muscle and on variations in the antioxidant indicators in liver and kidney. The OTC residual levels in muscle showed a dose–response relationship. After 10 days of treatment at the recommended dose (75 mg kg−1), the mean value in muscle was 295 μg kg−1. After 10 withdrawal days, residues in all treated groups were not entirely eliminated by fish. Residues of recommended 75 mg kg−1 OTC dose were lower than the maximum permitted by EEC regulation: 100 μg kg−1. Disturbance in the antioxidant systems in liver and kidney was recorded in (150, 300 mg kg−1) carp, as well as during the withdrawal period. A lowered superoxide dismutase activity and higher levels of catalase, glutathione peroxidase, glutathione reductase and glutathione were evaluated in liver, while in kidney only higher malondialdehyde and glutathione S-transferase concentrations were recorded for 300 mg kg−1 dose. The therapeutic OTC dose exerted lower effects, and only in liver, enhancement of GPx and GR activities was recorded. After the withdrawal period, altered antioxidant responses in tissues were restored for all three OTC doses.

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Acknowledgments

The work was financially supported by “Ministero della Salute” and co-financed by “Fondo Sociale Europeo (FSE) nell’ambito del Programma Operativo Regionale (POR) Umbria, FSE Obiettivo Competitività Regionale e Occupazione 2007–2013,” Italy.

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Authors and Affiliations

  1. Department of Cellular and Environmental Biology, University of Perugia, 06123, Perugia, Italy

    Antonia Concetta Elia, Nicole Pacini & Ambrosius Josef Martin Dörr

  2. C.Re.A.A. National Reference Centre for the Surveillance and Monitoring of Animal Feed, State Veterinary Institute, 10154, Turin, Italy

    Valentina Ciccotelli, Marilena Gili & Maria Cesarina Abete

  3. Fish Breeding Centre of Lake Trasimeno, Province of Perugia, 06123, Perugia, Italy

    Mauro Natali

  4. Department of Agricultural, Forest, and Food Sciences, University of Turin, 10095, Turin, Italy

    Laura Gasco

  5. Fish Disease Laboratory, State Veterinary Institute, 10154, Turin, Italy

    Marino Prearo

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  1. Antonia Concetta Elia
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  2. Valentina Ciccotelli
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  4. Ambrosius Josef Martin Dörr
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  9. Maria Cesarina Abete
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Correspondence to Antonia Concetta Elia.

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Elia, A.C., Ciccotelli, V., Pacini, N. et al. Transferability of oxytetracycline (OTC) from feed to carp muscle and evaluation of the antibiotic effects on antioxidant systems in liver and kidney. Fish Physiol Biochem 40, 1055–1068 (2014). https://doi.org/10.1007/s10695-013-9905-4

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