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Plasma biochemical responses of the omnivorous crucian carp (Carassius auratus) to crude cyanobacterial extracts

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Abstract

Healthy crucian carp (Carassius auratus) were treated by intraperitoneal (i.p.) injection of crude cyanobacterial extracts at two doses, 50 and 200 μg MC-LR equiv kg−1 BW. High mortality (100%) was observed within 60 h post injection in the high-dose group. In the treated fish, activities of four plasma enzymes, alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH), all showed substantial increases, with both dose and time-dependent effects. These increases of enzyme activity indicate severe impairment occurred in the liver of crucian carp over time. Plasma concentrations of energy-related biomolecules including glucose (GLU), cholesterol (CHO), triglyceride (TG), and total protein (TP) showed marked changes in the high-dose group, possibly a nutritional imbalance correlated with the liver injury caused by intraperitoneal exposure to crude cyanobacterial extracts.

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Acknowledgements

We would like to express our sincere thanks to Dr H.J.T. Goos and the anonymous reviewer for their useful comments and suggestions on the manuscript. This study is supported by a fund for lecture professor from Huazhong Agricultural University and the National Natural Science Foundation of China (30530170).

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Authors and Affiliations

  1. Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Fisheries College of Huazhong Agricultural University, Wuhan, 430070, P.R. China

    Xuezhen Zhang, Ping Xie, Weimin Wang & Dapeng Li

  2. Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, P.R. China

    Ping Xie & Dapeng Li

  3. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Jinzhou, 434000, P.R. China

    Zechao Shi

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  1. Xuezhen Zhang
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  2. Ping Xie
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  3. Weimin Wang
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  4. Dapeng Li
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Correspondence to Ping Xie.

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Zhang, X., Xie, P., Wang, W. et al. Plasma biochemical responses of the omnivorous crucian carp (Carassius auratus) to crude cyanobacterial extracts. Fish Physiol Biochem 34, 323–329 (2008). https://doi.org/10.1007/s10695-007-9191-0

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  • DOI: https://doi.org/10.1007/s10695-007-9191-0

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