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Marker assisted introgression of genes governing resistance to bacterial blight and blast diseases into an elite Basmati rice variety, ‘Pusa Basmati 1509’

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In the present study, we introgressed two genes each governing resistance to major diseases of rice namely, bacterial blight (BB) (xa13 and Xa21) and blast (Pi2 and Pi54) into a popular Basmati cultivar, Pusa Basmati 1509 (PB 1509) through marker assisted backcross breeding (MABB). Through foreground selection, seven plants homozygous for all the four genes were selected from a large population of 1832 BC2F2 plants and subjected to background selection coupled with phenotypic selection for agronomic and grain quality traits of the recurrent parent. BC2F2 selections were further advanced to BC2F4 generation to develop near-isogenic lines (NILs). Six NILs from the BC2F4 families with an RPG recovery ranging from 82.5% to 90.5% were evaluated in multi-location trials for agronomic performance, grain quality traits and disease resistance. The level of resistance to BB and blast diseases in all the selected NILs was similar to that of the donor parent. Three BB races, race 2, 4 and 6, respectively produced on average 1.8, 2.3 and 2.5 cm of lesion length in the NILs as against 15.6, 18.1 and 20.8 cm in PB1509. Further, the NILs recorded a disease score of 1.0 and 1.7 for two blast isolates Mo-nwi-38 and Mo-nwi-kas, respectively, as against the score of 4.0 in PB 1509 for both the isolates. The NILs were similar to PB 1509 for major agronomic and grain quality traits with the advantage of resistance to BB and blast diseases.

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Acknowledgements

This work is part of the Ph.D. programme of the first author. First author sincerely acknowledges Division of Genetics, ICAR-IARI for providing all the infrastructure and facilities for research. He also acknowledges the help rendered by the technical and supporting staff of the rice section, Division of Genetics and RBGRC, ICAR-IARI, Aduthurai for carrying out the field experiments.

Funding

The study received fellowship support from the Post-Graduate school, ICAR-IARI, New Delhi, University Grants Commission and Department of Science & Technology, Govt. of India.

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Author notes

  1. Vidya Sagar

    Present address: Division of Crop Improvement, ICAR-Indian Institute of Vegetable Research, Varanasi, 221305, India

Authors and Affiliations

  1. Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Vidya Sagar, Gaurav Dhawan, S. Gopala Krishnan, K. K. Vinod, Ranjith K. Ellur, Prolay K. Bhowmick, Haritha Bollinedi & Ashok Kumar Singh

  2. Rice Breeding and Genetics Research Centre, ICAR-Indian Agricultural Research Institute, Aduthurai, 612101, India

    M. Nagarajan

  3. Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Kalyan K. Mondal & G. Prakash

  4. Department of Agricultural Biotechnology, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur, Himachal Pradesh, 176062, India

    Rajeev Rathour

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  1. Vidya Sagar
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Authors’ contribution

AKS and GKS conceptualised the project; AKS and GKS led the experiment and did evaluation and mid-course corrections; VS, GD, RKE, MN and GKS generated the plant materials and conducted the field experiments; VS, KKM, GP and RR performed the disease screening experiments; VS, GKS, PKB, HB and KKV did the data curation and analyses; VS, GKS, KKV and AKS wrote the paper. All the authors have read and approved the final manuscript.

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Sagar, V., Dhawan, G., Gopala Krishnan, S. et al. Marker assisted introgression of genes governing resistance to bacterial blight and blast diseases into an elite Basmati rice variety, ‘Pusa Basmati 1509’. Euphytica 216, 16 (2020). https://doi.org/10.1007/s10681-019-2549-4

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