Abstract
Ranbir Basmati, an early maturing pure line selection of traditional Basmati 370, is widely cultivated Basmati variety in the Indian state of Jammu and Kashmir. The variety is tall statured, prone to lodging and highly susceptible to rice blast. The present study was carried out for genetic improvement of Ranbir Basmati for semi dwarf stature and resistance to blast by introgressing semi-dwarfing gene, sd1, and blast resistance gene, Pi9 from an improved Basmati rice variety, Pusa Basmati 1637 (PB 1637).The selfed progenies of two superior BC2F1 recombinants of the cross Ranbir Basmati/PB 1637 showing maximum recovery of recurrent parent genome and phenome were analyzed with gene-based markers to select plants homozygous for both Pi9 and sd1. These plants were processed via anther culture to produce homozygous doubled haploid (DH) lines. All the anther culture derived DH plants were short-statured compared to Ranbir Basmati and exhibited high level of resistance to blast. The analysis of yield and its component traits revealed the presence of superior transgressive segregants for various traits namely, effective tillers, grains per panicle and yield per plant. Combined use of marker-assisted selection and anther culture ensured speedy conversion of Ranbir Basmati into semi-dwarf genotypes with in-built resistance to rice blast. The study demonstrated the utility of anther culture as an adjunct to marker-assisted backcross breeding schemes for instant elimination of the residual background heterozygosity of the selected recombinants.
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References
Amante-Bordeos A, Sitch LA, Nelson R, Dalmacio RD, Oliva NP, Aswidinnoor H, Leung H (1992) Transfer of bacterial blight and blast resistance from the tetraploid wild rice Oryza minuta to cultivated rice, Oryza sativa. Theor Appl Genet 84(4):345–354
Amarawathi Y, Singh R, Singh AK, Singh VP, Mohapatra T, Sharma TR, Singh NK (2008) Mapping of quantitative trait loci for basmati quality traits in rice (Oryza sativa L.). Mol Breed 21(1):49–65
APEDA (2017) Basmati acreage and production. Basmati export development foundation, agricultural and processed food products export development authority (APEDA), New Delhi, Report–6. p 4
Basavaraj SH, Singh VK, Singh A, Singh A, Singh A, Anand D, Yadav S, Ellur RK, Singh D, Krishnan SG, Nagarajan M (2010) Marker-assisted improvement of bacterial blight resistance in parental lines of Pusa RH10, a superfine grain aromatic rice hybrid. Mol Breed 26(2):293–305
Bhatia D, Sharma R, Vikal Y, Mangat GS, Mahajan R, Sharma N, Lore JS, Singh N, Bharaj TS, Singh K (2011) Marker-assisted development of bacterial blight resistant, dwarf, and high yielding versions of two traditional basmati rice cultivars. Crop Sci 51(2):759–770
Bishnoi U, Jain RK, Rohilla JS, Chowdhury VK, Gupta KR, Chowdhury JB (2000) Anther culture of recalcitrant indica × Basmati rice hybrids. Euphytica 114(2):93–101
Chen CC, Wu YH (1983) Segmentations in microspores of rice during anther culture. Proc Natl Sci Counc ROC 7B:151–157
Chu CC, Wang CS, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sin 18(5):659–668
Ellis MH, Spielmeyer W (2002) Perfect markers for the semi dwarfing gene sd-1 in rice. IRRI Notes 27(2):13–14
Ellur RK, Khanna A, Yadav A, Pathania S, Rajashekara H, Singh VK, Krishnan SG, Bhowmick PK, Nagarajan M, Vinod KK, Prakash G (2016) Improvement of Basmati rice varieties for resistance to blast and bacterial blight diseases using marker assisted backcross breeding. Plant Sci 242:330–341
Germana MA (2011) Anther culture for haploid and doubled haploid production. Plant Cell, Tissue Organ Cult 104(3):283–300
Glaszmann JC (1987) Isozymes and classification of Asian rice varieties. Theor Appl Genet 74(1):21–30
Guiderdoni E, Galinato E, Luistro J, Vergara G (1992) Anther culture of tropical japonica × indica hybrids of rice (Oryza sativa L.). Euphytica 62(3):219–224
Hedden P (2003) The genes of the green revolution. Trends Genet 19(1):5–9
Imam J, Alam S, Mandal NP, Variar M, Shukla P (2014) Molecular screening for identification of blast resistance genes in North East and Eastern Indian rice germplasm (Oryza sativa L.) with PCR based makers. Euphytica 196(2):199–211
IRRI (2002) Standard evaluation system for rice. International Rice Research Institute, Manila
Juliano BO (1971) A simplified assay for milled rice amylose. Cereal Sci Today 16(10):334–340
Kaushal L, Balachandran SM, Ulaganathan K, Shenoy V (2014) Effect of culture media on improving anther culture response of rice (Oryza sativa L.). Int J Agric Innov Res 3(1):218–224
Khanna A, Sharma V, Ellur RK, Shikari AB, Krishnan SG, Singh UD, Prakash G, Sharma TR, Rathour R, Variar M, Prashanthi SK (2015) Development and evaluation of near-isogenic lines for major blast resistance gene(s) in Basmati rice. Theor Appl Genet 128(7):1243–1259
Khush GS (1995) Breaking the yield frontier of rice. Geo Journal 35(3):329–332
Kumar I, Khush GS (1986) Genetics of amylose content in rice (Oryza sativa L.). J Genet 65(2):1–11
Lanceras JC, Huang Z, Naivikul O, Vanavichit A, Ruanjaichon V, Tragoonrung S (2000) Mapping of genes for cooking and eating qualities in Thai Jasmine rice (KDML105). DNA Res 7(2):93–101
Lee YT, Lim MS, Kim HS, Shin HT, Kim CH, Bae SH, Cho CI (1989) An anther-derived new high quality rice variety with disease and insect resistance” Hwacheongbyeo”. Res Rep Rural Dev Admin-Rice 31(2):27–34
Little RR, Hilder GB, Dawson EH (1958) Differential effect of dilute alkali on 25 varieties of milled white rice. Cereal Chem 35(2):111–126
Lorieux M, Petrov M, Huang N, Guiderdoni E, Ghesquiere A (1996) Aroma in rice: genetic analysis of a quantitative trait. Theor Appl Genet 93(7):1145–1151
Luo Y, Yin Z (2013) Marker-assisted breeding of Thai fragrance rice for semi-dwarf phenotype, submergence tolerance and disease resistance to rice blast and bacterial blight. Mol Breed 32(3):709–721
Ma J, Lei C, Xu X, Hao K, Wang J, Cheng Z, Ma X, Ma J, Zhou K, Zhang X, Guo X, Wu F, Lin Q, Wang C, Zhai H, Wang H, Wan J (2015) Pi-64, encoding a novel CC-NBS-LRR protein, confers resistance to leaf and neck blast in rice. Mole Plant Microbe Interact 28(5):558–568
Mackill DJ, Bonman JM (1992) Inheritance of blast resistance in near-isogenic lines of rice. Phytopathology 82(7):746–749
Monna L, Kitazawa N, Yoshino R, Suzuki J, Masuda H, Maehara Y, Tanji M, Sato M, Nasu S, Minobe Y (2002) Positional cloning of rice semi dwarfing gene, sd-1: rice “green revolution gene” encodes a mutant enzyme involved in gibberellin synthesis. DNA Res 9(1):11–17
Murai M, Takamure I, Sato S, Tokutome T, Sato Y (2002) Effects of the dwarfing gene originating from ‘Dee-geo-woo-gen’ on yield and its related traits in rice. Breed Sci 52(2):95–100
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15(3):473–497
Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8(19):4321–4325
Nagaraju J, Kathirvel M, Kumar RR, Siddiq EA, Hasnain SE (2002) Genetic analysis of traditional and evolved Basmati and non-Basmati rice varieties by using fluorescence-based ISSR-PCR and SSR markers. Proc Natl Acad Sci USA 99(9):5836–5841
Patil VD, Nerkar YS, Misal MB, Harkal SR (1997) Parag 401, a semi-dwarf rice variety developed through anther culture. IRRI Notes 22(2):1
Pauk J, Janeso M, Simon-Kiss I (2009) Rice doubled haploids and breeding. In: Touraev A, Forster BP, Jain SM (eds) Advances in haploid production in higher plants. Springer, Berlin, pp 189–197
Pinthus MJ (1974) Lodging in wheat, barley, and oats: the phenomenon, its causes, and preventive measures. Adv Agron 25:209–263
Qu S, Liu G, Zhou B, Bellizzi M, Zeng L, Han B, Wang GL (2006) The broad-spectrum blast resistance gene Pi-9 encodes a nucleotide-binding site–leucine-rich repeat protein and is a member of a multigene family in rice. Genetics 172(3):1901–1914
Raina SK, Zapata FJ (1997) Enhanced anther culture efficiency of indica rice (Oryza sativa L.) through modification of the culture media. Plant Breed 116(4):305–315
Raina SK, Irfan ST (1998) High-frequency embryogenesis and plantlet regeneration from isolated microspores of Indica rice. Plant Cell Rep 17:957–962
Raina SK, Sathish P, Sarma KS (1987) Plant regeneration from in vitro cultures of anthers and mature seeds of rice (Oryza sativa L.) cv. Basmati-370. Plant Cell Rep 6(1):43–45
Rajpurohit D, Kumar R, Kumar M, Paul P, Awasthi A, Basha PO, Puri A, Jhang T, Singh K, Dhaliwal HS (2011) Pyramiding of two bacterial blight resistance and a semi dwarfing gene in Type 3 Basmati using marker-assisted selection. Euphytica 178(1):111–126
Rani NS, Singh RK (2003) Efforts on aromatic rice improvement in India. In: Singh RK, Singh US (eds) A treatise on the scented rices of India. Kalyani Publishers, New Delhi, pp 23–72
Rathour R, Singh BM, Sharma TR (2004) Population structure of Magnaporthe grisea from north western Himalayas and its implications for blast resistance breeding of rice. J Phytopathol 152(5):304–312
Rathour R, Katoch A, Kusum Kaushik RP, Sharma TR (2011) Virulence analysis of Magnaporthe oryzae for resistance gene deployment in north-western Himalayas. Plant Dis Res 26(2):183
Rathour R, Krishnan SG, Khanna A, Dhatwalia S, Kachra A, Sharma TR, Singh AK (2016) Development and validation of co-dominant gene based markers for Pi-9, a gene governing broad-spectrum resistance against blast disease in rice. Mol Breed 36(12):168
Roy B, Mandal AB (2005) Anther culture response in indica rice and variations in major agronomic characters among the androclones of a scented cultivar, Karnal local. Afr J Biotechnol 4(2):235–240
Senadhira D, Zapata-Arias FJ, Gregorio GB, Alejar MS, De LaCruz HC, Padolina TF, Galvez AM (2002) Development of the first salt tolerant rice cultivar through indica/indica anther culture. Field Crops Res 76(3):103–110
Singh P (2014) Development of bacterial blight resistant basmati rice doubled haploids through anther culture. M.Sc. thesis. School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, India
Singh RK, Singh US, Kush GS (2000) Aromatic rices. Oxford & IBH Publishing Co. Pvt. Ltd., New Delhi
Singh VK, Singh A, Singh SP, Ellur RK, Choudhary V, Sarkhel S, Singh D, Gopala Krishnan S, Nagarajan M, Vinod KK, Singh UD, Rathore R, Prasanthi SK, Agrawal PK, Bhatt JC, Mohapatra T, Prabhu KV, Singh AK (2012) Incorporation of blast resistance into “PRR78”, an elite Basmati rice restorer line, through marker assisted backcross breeding. Field Crops Res 128:8–16
Singh AK, Gopala Krishnan S, Nagarajan M, Bhowmick PK, Ellur RK, Haritha B, Vinod KK, Prabhu KV, Khanna A, Singh UD, Sharma TR, Prakash G, Seth R, Kumar D (2017) Notification of Basmati rice variety Pusa Basmati 1637. Indian J Genet 77(4):583–584
Singh V, Singh AK, Mohapatra T, Gopala Krishnan S, Ellur RK (2018) Pusa Basmati 1121—a rice variety with exceptional kernel elongation and volume expansion after cooking. Rice 11:19. https://doi.org/10.1186/s12284-018-0213-6
Snape JW (1989) Doubled haploid breeding: theoretical basis and practical applications. In: Mujeeb-Kazi A, Sitch LA (eds) Review of advances in plant biotechnology, 1985–1988: 2nd international symposium on genetic manipulation in crops. CIMMYT and IRRI, Mexico and Manila, pp 19–30
Sood BC, Siddiq EA (1978) A rapid technique for scent determination in rice. Indian J Genet Plant Breed 38(2):268–275
Spielmeyer W, Ellis MH, Chandler PM (2002) Semidwarf (sd-1), “green revolution” rice, contains a defective gibberellin 20-oxidase gene. Proc Natl Acad Sci USA 99(13):9043–9048
Takemoto-Kuno Y, Mitsueda H, Suzuki K, Hirabayashi H, Ideta O, Aoki N, Umemoto T, Ishii T, Ando I, Kato H, Nemoto H (2015) qAC2, a novel QTL that interacts with Wx and controls the low amylose content in rice (Oryza sativa L.). Theor Appl Genet 128(4):563–573
Thakur S, Gupta YK, Singh PK, Rathour R, Variar M, Prashanthi SK (2013) Molecular diversity in rice blast resistance gene Pi-ta makes it highly effective against dynamic population of Magnaporthe oryzae. Funct Integr Genomics 13:309–322
Tian D, Chen Z, Chen Z, Zhou Y, Wang Z, Wang F, Chen S (2016) Allele-specific marker-based assessment revealed that the rice blast resistance genes Pi-2 and Pi-9 have not been widely deployed in Chinese indica rice cultivars. Rice 9(1):19
Tomita M, Ishii K (2018) Genetic performance of the semi dwarfing allele sd-1 derived from a japonica rice cultivar and minimum requirements to detect its single-nucleotide polymorphism by miSeq whole-genome sequencing. Biomed Res Int. https://doi.org/10.1155/2018/4241725
Trijatmiko KR, Prasetiyono J, Thomson MJ, Cruz CM, Moeljopawiro S, Pereira A (2014) Meta-analysis of quantitative trait loci for grain yield and component traits under reproductive-stage drought stress in an upland rice population. Mol Breed 34(2):283–295
Xu X, Hayashi N, Wang CT, Fukuoka S, Kawasaki S, Takasuji H, Jiang CJ (2014) Rice blast resistance gene Pikahei-1(t), a member of a resistance gene cluster on chromosome 4, encodes a nucleotide-binding site and leucine-rich repeat protein. Mol Breed 34:691–700
Yan J, Xue Q, Zhu J (1996) Genetic studies of anther culture ability in rice (Oryza sativa). Plant Cell, Tissue Organ Cult 45:253–258
Yin KC, Hsu C, Chu CY, Pi FY, Wang ST, Liu TY, Chu CC, Wang CC, Sun CS (1976) A study of the new cultivar of rice raised by haploid breeding method. Sci Sin 19(2):227–242
Acknowledgements
The corresponding author gratefully acknowledges the financial support (San No. BT/PR10966/AGII/106/960/2014) received from the Department of Biotechnology, Govt. of India for the work reported in this paper.
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RR conceived and designed the experiments and wrote the final manuscript. PS performed MAS and anther culture experiments.TP performed crossing and background analysis. SGK and AKS helped in grain quality analysis. SGK, AKS and RKS also read and edited the manuscript.
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10681_2019_2473_MOESM1_ESM.pptx
Supplementary Fig. 1 Comparison of plant height of sd-1 homozygous doubled haploids with parental genotypes Ranbir Basmati and PB 1637 (PPTX 1194 kb)
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Samal, P., Pote, T.D., Krishnan, S.G. et al. Integrating marker-assisted selection and doubled haploidy for rapid introgression of semi-dwarfing and blast resistance genes into a Basmati rice variety ‘Ranbir Basmati’. Euphytica 215, 149 (2019). https://doi.org/10.1007/s10681-019-2473-7
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DOI: https://doi.org/10.1007/s10681-019-2473-7