Skip to main content
SpringerLink
Log in

QTL analysis reveals new eggplant loci involved in resistance to fungal wilts

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Fusarium spp. and Verticillium spp. are widespread soil pathogens responsible for vascular wilts causing heavy yield losses in eggplant (Solanum melongena) as well as in many other crops. Here we report on the identification of QTLs affecting the resistance to Fusarium and Verticillium in an F2 intraspecific population of 156 individuals bred from the cross ‘305E40’ × ‘67/3’, we previously characterized for key agronomic and biochemical traits. The female parent (‘305E40’) is an androgenetic introgressed line carrying the resistance locus Rfo-Sa1 derived from Solanum aethiopicum. The line is fully resistant to Fusarium and also displays a previously uncharacterized partial resistance to Verticillium. The male parent (‘67/3’) is an F8 selection from the eggplant intra-specific cross cv. ‘Purpura’ x cv. ‘CIN2’ which, unexpectedly, revealed a not previously characterized partial resistance to Fusarium, but it is highly susceptible to Verticillium. The degree of resistance of the F2 population was assessed following artificial inoculation in greenhouse (Fusarium) or growth chamber (Verticillium) of F2:3 progenies obtained by selfing each F2 individual. Other than a major QTL for the resistance to Fusarium, which lies in the genomic region of the Rfo-Sa1 locus, major and minor QTL influencing the response to both Fusarium and Verticillium were spotted, and putative tomato orthologous genes were identified as well. The markers linked to the spotted QTL may find application in the context of marker-assisted breeding.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Acciarri N, Rotino GL, Valentino D, Vitelli G, Sunseri F, Martelli G, Tamietti G (2001) Genetic improvement of eggplant for resistance to Verticillium through interspecific hybridization with Solanum sodomeum L. In Proceedings of the 11th Eucarpia meeting on genetics and breeding of capsicum and eggplant, pp. 298–302

  • Acciarri N, Rotino GL, Sabatini E, Valentino D, Sunseri F, Mennella G, Tamietti G (2004). Improvement of eggplants for resistance to Verticillium. In: Proceedings of the 12th Eucarpia meeting on genetics and breeding of capsicum and eggplant, p 178

  • Albert VA, Chang TH (2014) Evolution of a hot genome. Proc Natl Acad Sci USA 111(14):5069–5070. https://doi.org/10.1073/pnas.1402378111

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Altinok HH (2005) First report of fusarium wilt of eggplant caused by Fusarium oxysporum f. sp. melongenae in Turkey. Plant Pathol 54:577

    Article  Google Scholar 

  • Altınok HH, Can C (2010) Characterization of Fusarium oxysporum f. sp. melongenae isolates from eggplant in Turkey by pathogenicity, VCG and RAPD analysis. Phytoparasitica 38(2):149–157

    Article  Google Scholar 

  • Altinok HH, Can C, Çolak H (2013) Vegetative compatibility, pathogenicity and virulence diversity of Fusarium oxysporum f. sp. melongenae recovered from eggplant. J Phytopathol 1619:651–660

    Article  Google Scholar 

  • Altinok HH, Can C, Boyaci HF, Topcu V (2014) Genetic variability among breeding lines and cultivars of eggplant against Fusarium oxysporum f. sp. melongenae from Turkey. Phytoparasitica 421:75–84

    Article  Google Scholar 

  • Bae J, Halterman D, Jansky SH (2008) Development of a molecular marker associated with Verticillium wilt resistance in diploid interspecific potato hybrids. Mol Breed 22:61–69

    Article  CAS  Google Scholar 

  • Barbierato V, Toppino L, Rinaldi P, Sala T, Bassolino L, Valè G, Ferrarini A, Delledonne M, Bagnaresi P, Rotino GL (2016) Phenotype and gene expression analyses of the Rfo-sa1 resistant aubergine interaction with Fusarium oxysporum f. sp. melongenae and Verticillium dahliae. Plant Pathol 5(8):1297–1309. https://doi.org/10.1111/ppa.12518

    Article  Google Scholar 

  • Barchi L, Lanteri S, Portis E, Stagel A, Valè G, Toppino L, Rotino GL (2010) Segregation distortion and linkage analysis in eggplant (Solanum melongena L.). Genome 53:805–815

    Article  CAS  PubMed  Google Scholar 

  • Barchi L, Lanteri S, Portis E, Acquadro A, Valè G, Toppino L, Rotino GL (2011) Identification of SNP and SSR markers in eggplant using RAD tag sequencing. BMC Genomics. https://doi.org/10.1186/1471-2164-12-304

    PubMed  PubMed Central  Google Scholar 

  • Barchi L, Lanteri S, Portis E, Valè G, Volante A, Pulcini L, Ciriaci T, Acciarri N, Barbierato V, Toppino L, Rotino G (2012) A RAD Tag derived marker based eggplant linkage map and the location of QTLs determining anthocyanin pigmentation. PLoS ONE 7:e43740

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bhat RG, Subbarao KV (1999) Host range specificity in Verticillium dahliae. Phytopathology 8912:1218–1225

    Article  Google Scholar 

  • Bournival BL, Vallejos CE, Scott JW (1990) Genetic analysis of resistances to races 1 and 2 of Fusarium oxysporum f. sp. lycopersici from the wild tomato Lycopersicon pennellii. Theor Appl Genet 79(5):641–645

    Article  CAS  PubMed  Google Scholar 

  • Boyaci F, Unlu A, Abak K (2010) Screening for resistance to Fusarium wilt of some cultivated eggplants and wild Solanum accessions. In XXVIII International Horticultural Congress on Science and Horticulture for People IHC2010: International Symposium on New, pp 23–27

  • Boyaci HF, Unlu A, and Abak K (2011) Genetic analysis of resistance to wilt caused by Fusarium (Fusarium oxysporum melongenae) in eggplant (Solanum melongena). The Indian Journal of Agricultural Sciences, p 819

  • Cappelli C, Stravato VM, Rotino GL, Buonaurio R (1995) Sources of resistance among Solanum spp., to an Italian isolate of Fusarium oxysporum f. sp. melongenae, In: Andràsfalvi A, Moòr A, Zatykò (eds) EUCARPIA, 9th Meeting on Genet Breed Capsicum Eggplant, SINCOP, Budapest, pp 221–224

  • Cericola F, Portis E, Toppino L, Barchi L, Acciarri N, Ciriaci T, Sala T, Rotino GL, Lanteri S (2013) The population structure and diversity of eggplant from Asia and the Mediterranean Basin. PLoS ONE 8:e73702

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cericola F, Portis E, Lanteri S, Toppino L, Barchi L, Acciarri N, Pulcini L, Sala T, Rotino GL (2014) Linkage disequilibrium and genome-wide association analysis for anthocyanin pigmentation and fruit color in eggplant. BMC Genomics. https://doi.org/10.1186/1471-2164-15-896

    PubMed  PubMed Central  Google Scholar 

  • Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971

    CAS  PubMed  PubMed Central  Google Scholar 

  • Daunay (2008). Eggplant. Prohens J, Nuez F, editors. Handbook of Plant Breeding—Vegetables II. Springer, New York, pp 163–220

  • Daunay MC (2012) Eggplant. In: Peter KV, Hazra P (eds) Handbook of vegetables. Studium Press, Houston, pp 257–322

    Google Scholar 

  • Daunay MC, Lester RN, Laterrot H (1991). The use of wild species for the genetic improvement of Brinjal eggplant Solanum melongena and tomato Lycopersicon esculentum, In: Hawkes JC, Lester RN, Nee M, Estrada N (Eds). Solanaceae III: Taxonomy, Chemistry, Evolution, Vol 27. Royal Botanic Gardens Kew and Linnean Soc London, pp 389–413

  • Diwan N, Fluhr R, Eshed Y, Zamir D, Tanksley SD (1999) Mapping of Ve in tomato: a gene conferring resistance to the broad-spectrum pathogen, Verticillium dahliae race 1. Theor Appl Genet 982:315–319

    Article  Google Scholar 

  • Doglanar S, Frary A, Daunay MC, Lester RN, Tanksley SD (2002a) A comparative genetic linkage map of eggplant (Solanum melongena) and its implication for genome evolution in the Solanacee. Genetics 161:1697–1711

    Google Scholar 

  • Doglanar S, Frary A, Daunay MC, Lester RN, Tanksley SD (2002b) Conservation of gene function in the solanaceae as revealed by comparative mapping of domestication trait in eggplant. Genetics 161:1713–1726

    Google Scholar 

  • Fei J, Chai Y, Wang J, Lin J, Sun X, Sun C, Zuo K, Tang K (2004) cDNA cloning and characterization of the Ve homologue gene StVe from Solanum torvum Swartz. Mitochondrial DNA 15(2):88–95

    CAS  Google Scholar 

  • Fowler C, Moore GK, Hawtin G (2003) The international treaty on plant genetic resources for food and agriculture. Bioversity International, Rome

  • Fradin EF, Zhang Z, Ayala JCJ, Castroverde CD, Nazar RN, Robb J, Liu CM, Thomma BP (2009) Genetic dissection of Verticillium wilt resistance mediated by tomato Ve1. Plant Physiol 150(1):320–332

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Frary A, Doganlar S, Daunay MC, Tanksley SD (2003) QTL analysis of morphological traits in eggplant and implications for conservation of gene function during evolution of solanaceous species. Theor Appl Genet 107:359–370

    Article  CAS  PubMed  Google Scholar 

  • Frary A, Frary A, Daunay MC, Huvenaars K, Mank R, Doğanlar S (2014) QTL hotspots in eggplant (Solanum melongena) detected with a high resolution map and CIM analysis. Euphytica 197(2):211–228. https://doi.org/10.1007/s10681-013-1060-6

    Article  Google Scholar 

  • Fukuoka H, Yamaguchi H, Nunome T, Negoro S, Miyatake K, Ohyama A (2010) Accumulation, functional annotation, and comparative analysis of expressed sequence tags in eggplant (Solanum melongena L.), the third pole of the genus Solanum species after tomato and potato. Gene 450:76–84. https://doi.org/10.1016/j.gene.2009.10.006

    Article  CAS  PubMed  Google Scholar 

  • Fukuoka H, Miyatake K, Nunome T, Negoro S, Shirasawa K, Isobe S, Asamizu E, Yamaguchi H, Ohyama A (2012) Development of gene-based markers and construction of an integrated linkage map in eggplant by using Solanum orthologous (SOL) gene sets. Theor Appl Genet 125(1):47–56. https://doi.org/10.1007/s00122-012-1815-9

    Article  CAS  PubMed  Google Scholar 

  • Fukuoka S, Yamamoto SI, Mizobuchi R, Yamanouchi U, Ono K, Kitazawa N, Yasuda N, Fujiita Y, Thanh Nguyen TT, Koizumi S, Sugimoto K, Matsumoto T, Yano M (2014) Multiple functional polymorphisms in a single disease resistance gene in rice enhance durable resistance to blast. Scientific Reports 4, (article number 4550) https://doi.org/10.1038/srep04550

  • Fukuoka S, Saka N, Mizukami Y, Koga H, Yamanouchi U, Yoshioka Y, Hayashi N, Ebana K, Mizobuchi R, Yano M (2015) Gene pyramiding enhances durable blast disease resistance in rice. Sci Rep 5:7773

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Goggin FL, Jia L, Shah G, Hebert S, Williamson VM, Ullman DE (2006) Heterologous expression of the Mi-1.2 gene from tomato confers resistance against nematodes but not aphids in eggplant. Mol Plant Microbe Interact 19(4):383–388

    Article  CAS  PubMed  Google Scholar 

  • Gramazio P, Prohens J, Plazas M, Andújar I, Herraiz FJ, Castillo E, Knapp S, Meyer RS, Vilanova S (2014) Location of chlorogenic acid biosynthesis pathway and polyphenol oxidase genes in a new interspecific anchored linkage map of eggplant. BMC Plant Biol 14(1):1

    Article  Google Scholar 

  • Gramazio P, Blanca J, Ziarsolo P, Herraiz FJ, Plazas M, Prohens J, Vilanova S (2016) Transcriptome analysis and molecular marker discovery in Solanum incanum and S. aethiopicum, two close relatives of the common eggplant (Solanum melongena) with interest for breeding. BMC Genomics 17(1):1

    Article  Google Scholar 

  • Hayes RJ, McHale LK, Vallad GE, Truco MJ, Michelmore RW, Klosterman SJ, Maruthachalam K, Subbarao KV (2011) The inheritance of resistance to Verticillium wilt caused by race 1 isolates of Verticillium dahliae in the lettuce cultivar La Brillante. Theor Appl Genet 123:509–517

    Article  CAS  PubMed  Google Scholar 

  • Hemming MN, Basuki S, McGrath DJ, Carroll BJ, Jones DA (2004) Fine mapping of the tomato I-3 gene for fusarium wilt resistance and elimination of a co-segregating resistance gene analogue as a candidate for I-3. Theor Appl Genet 109:409–418

    Article  CAS  PubMed  Google Scholar 

  • Hirakawa H, Shirasawa K, Miyatake K, Nunome T, Negoro S, Ohyama A, Yamaguchi H, Sato S, Isobe S, Tabata S, Fukuoka H (2014) Draft genome sequence of eggplant (Solanum melongena L): the representative solanum species indigenous to the old world. DNA Res 216:649–660

    Article  Google Scholar 

  • Jansen R (1993) Interval mapping of multiple quantitative trait loci. Genetics 135:205–211

    CAS  PubMed  PubMed Central  Google Scholar 

  • Jansen R, Stam P (1994) High-resolution of quantitative traits into multiple loci via interval mapping. Genetics 136:1447–1455

    CAS  PubMed  PubMed Central  Google Scholar 

  • Karagiannidis N, Bletsos F, Stavropoulos N (2002) Effect of Verticillium wilt (Verticillium dahliae Kleb.) and mycorrhiza (Glomus mosseae) on root colonization, growth and nutrient uptake in tomato and eggplant seedlings. Sci Hort. 94:145–156

    Article  CAS  Google Scholar 

  • Kaushik P, Prohens J, Vilanova S, Gramazio P, Plazas M (2016) Phenotyping of eggplant wild relatives and interspecific hybrids with conventional and phenomics descriptors provides insight for their potential utilization in breeding. Front Plant Sci 7:677

    Article  PubMed  PubMed Central  Google Scholar 

  • Kawashima CG, Guimarães GA, Nogueira SR, MacLean D, Cook DR, Steuernagel B, Baek J, Bouyioukos C, do VA Melo B, Tristão G, de Oliveira JC, Rauscher G, Mittal S, Panichelli L, Bacot K, Johnson J, Iyer G, Tabor G, Wulff BBH, Ward E, Rairdan GJ, Broglie KE, Wu G, van Esse1 HP, Jones JDJ, Brommonschenkel SH (2016). A pigeonpea gene confers resistance to Asian soybean rust in soybean. Nat Biotechnol 34:661–665

  • Kawchuk LM, Hachey J, Lynch DR, Kulcsar F, Van Rooijen G, Waterer DR, Robertson A, Kokko E, Byers R, Howard RJ, Fischer R, Prüfer D (2001) Tomato Ve disease resistance genes encode cell surface-like receptors. Proc Natl Acad Sci 98(11):6511–6515

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • King SR, Davis AR, Zhang X, Crosby K (2010) Genetics, breeding and selection of rootstocks for Solanaceae and Cucurbitaceae. Sci Hortic 127(2):106–111

    Article  Google Scholar 

  • Knapp S, Vorontsova MS, Prohens J (2013) Wild relatives of the eggplant (Solanum melongena L.: Solanaceae): new understanding of species names in a complex group. PLoS ONE. https://doi.org/10.1371/journal.pone.0057039

    Google Scholar 

  • Komochi S, Monma S, Narikawa T, Sakata Y (1996) Evaluation of resistance to bacterial wilt and verticillium wilt in eggplants (Solanum melongena L.) collected in Malaysia. J Jpn Soc Hortic Sci 65:81–88

    Article  Google Scholar 

  • Krzywinski M, Schein J, Birol I, Connors J, Gascoyne R, Horsman D, Jones SJ, Marra MA (2009) Circos: an information aesthetic for comparative genomics. Genome Res 19(9):1639–1645

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lander E, Botstein D (1989) Mapping mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199

    CAS  PubMed  PubMed Central  Google Scholar 

  • Lester RN and Hasan SMZ (1991). Origin and domestication of the brinjal eggplant, Solanum melongena, from S. incanum, in Africa and Asia. In Hawkes JG, Lester RN, Nee M, Estrada N eds. Solanaceae III: taxonomy, chemistry, evolution. R Bot Gard: Kew & Linnean Soc., London, pp 369–387

  • Lim GT, Wang GP, Hemming MN, McGrath DJ, Jones DA (2008) High resolution genetic and physical mapping of the I-3 region of tomato chromosome 7 reveals almost continuous microsynteny with grape chromosome 12 but interspersed microsynteny with duplications on Arabidopsis chromosomes 1, 2 and 3. Theor Appl Genet 118:57–75

    Article  CAS  PubMed  Google Scholar 

  • Liu J, Zheng Z, Zhou X, Feng C, Zhuang Y (2015) Improving the resistance of eggplant Solanum melongena to Verticillium wilt using wild species Solanum linnaeanum. Euphytica 2013:463–469

    Article  Google Scholar 

  • McKeen CD (1972) Observations on the invasion and subsequent development of Verticillium albo-atrum and Verticillium dahliae in eggplant, tomato and potato. Proc Can Phytopathol Soc 39:21

    Google Scholar 

  • Meyer RS, Karol KG, Little DP, Nee MH, Litt A (2012) Phylogeographic relationships among Asian eggplants and new perspectives on eggplant domestication. Mol Phylogenet Evol 63:685–701

    Article  PubMed  Google Scholar 

  • Michielse CB, Rep M (2009) Pathogen profile update: Fusarium oxysporum. Mol Plant Pathol 103:311–324

    Article  Google Scholar 

  • Miyatake K, Saito T, Negoro S, Yamaguchi H, Nunome T, Ohyama A, Fukuoka H (2012) Development of selective markers linked to a major QTL for parthenocarpy in eggplant Solanum melongena L. Theor Appl Genet 124:1403–1413

    Article  PubMed  Google Scholar 

  • Miyatake K, Saito T, Negoro S, Yamaguchi H, Nunome T, Ohyama A, Fukuoka H (2016) Detailed mapping of a resistance locus against Fusarium wilt in cultivated eggplant Solanum melongena. Theor Appl Genet 129(2):357–367. https://doi.org/10.1007/s00122-015-2632-8

    Article  PubMed  Google Scholar 

  • Monma S, Sato T, Matsunaga H (1996) Evaluation of resistance to bacterial Fusarium and Verticillium wilt in eggplant and eggplant-related species collected in Ghana. Capsicum Eggplant Nwsl 15:71–72

    Google Scholar 

  • Monma S, Akazawa S, Simosaka K, Sakata Y, Matsunaga H (1997) ‘Daitaro’, a bacterial wilt- and Fusarium wilt-resistant hybrid eggplant for rootstock. Bull Natl Inst Veg Ornam Plants Tea 12:73–83 (in Japanese with English summary)

    Google Scholar 

  • Mutlu N, Boyaci FH, Göçmen M, Abak K (2008) Development of SRAP, SRAP-RGA, RAPD and SCAR markers linked with a Fusarium wilt resistance gene in eggplant. Theor Appl Genet 117:1303–1312

    Article  CAS  PubMed  Google Scholar 

  • Nunome T, Ishiguro K, Yoshida T, Hirai M (2001) Mapping of fruit shape and color development traits in eggplant (Solanum melongena L.) based on RAPD and AFLP markers. Breed Sci 51:19–26

    Article  CAS  Google Scholar 

  • Nunome T, Suwabe K, Iketani H, Hirai M (2003) Identification and characterization of microsatellites in eggplant. Plant Breed 122:256–262

    Article  CAS  Google Scholar 

  • Nunome T, Negoro S, Kono I, Kanamori H, Miyatake K, Yamaguchi H, Ohyama A, Fukuoka H (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor Appl Genet 119:1143–1153

    Article  PubMed  Google Scholar 

  • Ori N, Eshed Y, Paran I, Presting G, Aviv D, Tanksley S, Zamir D, Fluhr R (1997) The I2C family from the wilt disease resistance locus I2 belongs to the nucleotide binding, leucine-rich repeat superfamily of plant resistance genes. Plant Cell 9:521–532

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Plazas M, Vilanova S, Gramazio P, Rodríguez-Burruezo A, Fita A, Herraiz FJ, Ranil R, Fonseka R, Niran L, Fonseka H, Kouassi B, Kouassi A, Kouassi A, Prohens J (2016) Interspecific hybridization between eggplant and wild relatives from different genepools. J Am Soc Hort Sci 141(1):34–44

    Google Scholar 

  • Portis E, Barchi L, Toppino L, Lanteri S, Acciarri N, Felicioni N, Fusari F, Barbierato V, Cericola F, Valè GP, Rotino GL (2014) QTL mapping in eggplant reveals clusters of yield-related loci and orthology with the tomato genome. PLoS ONE 9:e89499

    Article  PubMed  PubMed Central  Google Scholar 

  • Portis E, Cericola F, Barchi L, Toppino L, Acciarri N, Pulcini L, Sala T, Lanteri S, Rotino GL (2015) Association mapping for fruit, plant and leaf morphology traits in eggplant. PLoS ONE 108:e0135200

    Article  Google Scholar 

  • Rizza F, Mennella G, Collonnier C, Shiachakr D, Kashyap V, Rajam MV, Prestera M, Rotino GL (2002) Androgenic dihaploids from somatic hybrids between Solanum melongena and S. aethiopicum group gilo as a source of resistance to Fusarium oxysporum f. sp. melongenae. Plant Cell Rep 2011:1022–1032

    Google Scholar 

  • Rotino GL, Sihachakr D, Rizza F, Vale’ GP, Tacconi MG, Alberti P, Mennella G, Sabatini E, Toppino L, D’alessandro A, Acciarri N (2005) Current status in production and utilization of dihaploids from somatic hybrids between eggplant (Solanum melongena L), and its wild relatives. Acta Physiol Plant 274B:723–733

  • Rotino GL, Sala T, Toppino L (2014). Eggplant. Book chapter 16. In Pratap A, Kumar J (eds) Alien gene transfer in crop plants, vol 2, 381, Achievements and impacts. Springer, New York. https://doi.org/10.1007/978-1-4614-9572-7_16

  • Sarfatti M, Katan J, Fluhr R, Zamir D (1989) An RFLP marker in tomato linked to the Fusarium oxysporum resistance gene I2. Theor Appl Genet 78:755–759

    Article  CAS  PubMed  Google Scholar 

  • Sarfatti M, Abu-Abied M, Katan J, Zamir D (1991) RFLP mapping of I1, a new locus in tomato conferring resistance against Fusarium oxysporum f. sp. lycopersici race 1. Theor Appl Genet 82:22–26

    Article  CAS  PubMed  Google Scholar 

  • Segal G, Sarfatti M, Schaffer MA, Ori N, Zamir D, Fluhr R (1992) Correlation of genetic and physical structure in the region surrounding the I2 Fusarium oxysporum resistance locus in tomato. Mol Gen Genet 231:179–185

    CAS  PubMed  Google Scholar 

  • Sihachakr D, Daunay MC, Serraf L, Chaput MH, Mussio I, Haicour R, Rossignol L, Ducreux G (1994). Somatic hybridization of eggplant (Solanum melongena L), with its close and wilt relatives. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, somatic hybridization in crop improvement. Springer, Berlin

  • Simons G, Groenendijk J, Wijbrandi J, Reijans M, Groenen J, Diergaarde P, Van der Lee T, Bleeker M, Onstenk J, de Both M, Haring M, Mes J, Cornelissen B, Zabeau M, Vos P (1998) Dissection of the fusarium I2 gene cluster in tomato reveals six homologs and one active gene copy. Plant Cell 10:1055–1068

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Stravato VM, Cappelli C, Polverari A (1993) Attacchi di Fusarium oxysporum f, sp, melongenae agente della tracheofusariosi della melanzana in Italia centrale. Inf Fitopatol 4310:51–54

    Google Scholar 

  • Sunseri F, Sciancalepore A, Martelli G, Rotino GL, Acciarri N, Valentino D, Tamietti G (2003) Development of RAPD-AFLP map of eggplant and improvement of tolerance to Verticillium Wilt. Acta Hortic 625:107–115

    Article  CAS  Google Scholar 

  • Syfert M, Castañeda-Álvarez NP, Khoury CK, Särkinen T, Sosa CC, Achicanoy HA, Bernau V, Prohens J, Daunay MC, Knapp S (2016) Crop wild relatives of the brinjal eggplant (Solanum melongena): poorly represented in genebanks and many species at risk of extinction. Am J Bot 103:635-651

  • Team R (2009) R: a language and environment for statistical computing

  • Toppino L, Valè GP, Rotino GL (2008) Inheritance of Fusarium wilt resistance introgressed from Solanum aethiopicum Gilo and Aculeatum groups into cultivated eggplant S. melongena and development of associated PCR-based markers. Mol Breed 222:237–250. https://doi.org/10.1007/s11032-008-9170-x

    Article  Google Scholar 

  • Toppino L, Barchi L, Lo Scalzo R, Palazzolo E, Francese G, Fibiani M, D’Alessandro A, Papa V, Laudicina VA, Sabatino L, Pulcini L, Sala T, Acciarri N, Portis E, Lanteri S, Mennella G, Rotino GL (2016). Mapping Quantitative Trait Loci Affecting Biochemical and Morphological Fruit Properties in Eggplant (Solanum melongena L.) Front plant sci 7 (2016)

  • Uribe P, Jansky S, Halterman D (2014) Two CAPS markers predict Verticillium wilt resistance in wild Solanum species. Mol Breed 33(2):465–476

    Article  CAS  Google Scholar 

  • Urrutia Herrada MT, Gomez Garcia VM, Tello Marquina J (2004) Fusarium wilt on eggplant in Almeria (Spain). Boletin de Sanidad Vegetal, Plagas 30:85–92

    Google Scholar 

  • Van Ooijen JW (2004) MapQTL 5, software for the mapping of quantitative trait loci in experimental populations

  • van Ooijen J (2006) JoinMap H 4, Software for the calculation of genetic linkage maps in experimental populations. Kyazma BV, Wageningen, Netherlands

  • Villeneuve F, Latour F, Théry T, Steinberg C, Edel-Hermann V, Pitrat M, Daunay MC (2014) The control of soil borne vascular diseases: limits of genetic resistance of cultivars and rootstocks for controlling Fusarium oxysporum f, sp, melonis (melon) and Verticillium sp, (eggplant). Acta Hortic 1044:57–65

    Article  Google Scholar 

  • Vining K, Davis T (2009) Isolation of a Ve homolog, mVe1, and its relationship to verticillium wilt resistance in Mentha longifolia (L.) Huds. Mol Genet Genomics 282:173–184

    Article  CAS  PubMed  Google Scholar 

  • Wu F, Eannetta N, Xu Y, Tanksley S (2009) A detailed synteny map of the eggplant genome based on conserved ortholog set II (COSII) markers. Theor Appl Genet 118:927–935

    Article  CAS  PubMed  Google Scholar 

  • Yang L, Jue De, Li W, Zhang R, Chen M, Yang Q (2013) Identification of MiRNA from eggplant (Solanum melongena L), by small RNA deep sequencing and their response to Verticillium dahliae infection, PloS ONE vol, 8 8 p, e72840

  • Yang X, Cheng YF, Deng C, Ma Y, Wang ZW, Chen XH, Xue LB (2014) Comparative transcriptome analysis of eggplant (Solanum melongena L.) and turkey berry (Solanum torvum Sw.): phylogenomics and disease resistance analysis. BMC Genomics 15(1):412

    Article  PubMed  PubMed Central  Google Scholar 

  • Yoshida T, Monma S, Matsunaga H, Sakata Y, Sato T, Saito T (2004) Development of a new rootstock eggplant cultivar ‘Daizaburou’ with high resistance to bacterial wilt and Fusarium wilt. Bull Natl Inst Veg Tea Sci 3:199–211 (in Japanese with English summary)

    Google Scholar 

  • Zhang B, Yang Y, Chen T, Yu W, Liu T, Li H, Fan X, Ren Y, Shen D, Liu L, Dou D, Chang Y (2012) Island cotton Gbve1 gene encoding a receptor-like protein confers resistance to both defoliating and non-defoliating isolates of Verticillium dahliae. PLoS ONE 7(12):e51091

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhou X, Bao S, Liu J, Zhuang Y (2016) De novo sequencing and analysis of the transcriptome of the wild eggplant species Solanum Aculeatissimum in response to Verticillium dahliae. Plant Mol Biol Rep. https://doi.org/10.1007/s11105-016-0998-7

    Google Scholar 

Download references

Acknowledgements

This work has been funded in part by European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No 677379 (G2P-SOL project: Linking genetic resources, genomes and phenotypes of Solanaceous crops).

Author information

Author notes

  1. Barchi Lorenzo and Toppino Laura have equally contributed to the work.

Authors and Affiliations

  1. DISAFA Plant Genetics and Breeding, University of Torino, 10095, Grugliasco, Torino, Italy

    Lorenzo Barchi, Danila Valentino, Ezio Portis & Sergio Lanteri

  2. CREA, Research Centre for Genomics and Bioinformatics, 26836, Montanaso Lombardo, LO, Italy

    Laura Toppino, Laura Bassolino & Giuseppe Leonardo Rotino

Authors
  1. Lorenzo Barchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Laura Toppino
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Danila Valentino
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Laura Bassolino
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ezio Portis
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sergio Lanteri
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Giuseppe Leonardo Rotino
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Giuseppe Leonardo Rotino.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Barchi, L., Toppino, L., Valentino, D. et al. QTL analysis reveals new eggplant loci involved in resistance to fungal wilts. Euphytica 214, 20 (2018). https://doi.org/10.1007/s10681-017-2102-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s10681-017-2102-2

Keywords

Search

Navigation