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Fine mapping and candidate identification of SST, a gene controlling seedling salt tolerance in rice (Oryza sativa L.)

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Abstract

Using a recessive mutant with enhanced salt tolerance at seedling stage obtained from an indica rice cultivar R401 by gamma-ray irradiation, a novel gene controlling salt tolerance in rice was previously mapped to a 406-kb region on chromosome 6. We named the gene Seedling Salt Tolerance (SST). In this study, with a large F2 population derived from a cross between mutant sst and a japonica cultivar Nipponbare (salt sensitive), SST was further fine mapped to a 17-kb interval between InDel markers ID27101 and ID27118, in which only one gene (OsSPL10) was predicted. Sequencing analysis indicated that the 232nd base of the coding sequence of OsSPL10 was deleted in the sst allele, resulting in a frameshift mutation. The result strongly suggested that OsSPL10 should be the candidate of SST. OsSPL10 is a member of the SBP-box gene family. This is the first time that the SBP-box gene family is found to be probably involved in the regulation of seedling salt tolerance in plant.

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Acknowledgments

This work was supported financially by the National Natural Science Foundation of China (Grant No. 31071399), Fujian Natural Science Foundation (Grant No. 2011J01078), and Fujian Science and Technology Project (Grant No. JK2012014).

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    Authors and Affiliations

    1. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China

      Tao Lan, Shujun Zhang, Tingting Liu, Bin Wang, Huazhong Guan, Yuanchang Zhou, Yuanlin Duan & Weiren Wu

    2. Fujian Provincial Key Laboratory of Crop Breeding by Design, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China

      Tao Lan & Weiren Wu

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    1. Tao Lan
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    Correspondence to Weiren Wu.

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    Tao Lan and Shujun Zhang have contributed equally to this work.

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    Lan, T., Zhang, S., Liu, T. et al. Fine mapping and candidate identification of SST, a gene controlling seedling salt tolerance in rice (Oryza sativa L.). Euphytica 205, 269–274 (2015). https://doi.org/10.1007/s10681-015-1453-9

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