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Marker assisted selection of Ty-2 and Ty-3 carrying tomato lines and their implications in breeding tomato leaf curl disease resistant hybrids

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Abstract

Tomato leaf curl virus disease caused by whitefly-transmitted begomoviruses is a serious tomato production constraint in tropical and subtropical regions of the world. Use of genetic resistance for diseases caused by begomoviruses is an important component of sustainable disease management approaches. To date, six introgressions (Ty genes) derived from different tomato wild species that confer resistance to tomato leaf curl disease have been identified. Among these, dominant Ty-2 and partially dominant Ty-3 are important for the development of resistant hybrids because of their gene action. In the present study we report marker assisted selection of tomato lines carrying Ty-2 and Ty-3 genes and the effect of combining these in tomato hybrids using agroinoculation. The response of lines and hybrids with the Ty-2 gene indicated ineffectiveness of Ty-2 to infection by a bipartite tomato leaf curl virus. The Ty-3 gene showed dosage effect with partial resistance in heterozygous state. However, the level of resistance in hybrids with the combination of Ty-2 and Ty-3 genes was higher and similar to homozygous Ty-3 plants. The Ty-2 gene in either homozygous or heterozygous state enhanced the level of resistance of plants with heterozygous Ty-3. Thus, tomato hybrid breeding programs can be benefited by using tomato lines carrying Ty-2 and Ty-3 resistance genes. The combined results of evaluation of horticultural performance, field resistance and agroinoculation tests demonstrated that the set of tomato lines developed in this study could be useful resources for achieving higher level of resistance in tomato hybrids.

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References

  • Anbinder I, Reuveni M, Azari R, Paran I, Nahon S, Shlomo H, Chen L, Lapidot M, Levin I (2009) Molecular dissection of Tomato leaf curl virus resistance in tomato line TY172 derived from Solanum peruvianum. Theor Appl Genet 119:519–530

    Article  PubMed  Google Scholar 

  • Chakraborty S, Pandey PK, Banerjee MK, Kalloo G, Fauquet CM (2003) Tomato leaf curl Gujarat virus, a new begomovirus species causing severe leaf curl disease of tomato in Varanasi, India. Phytopathology 93:1485–1495

    Article  CAS  PubMed  Google Scholar 

  • Chatchawankanphanich O, Chiang BT, Green SK, Singh SJ, Maxwell DP (1993) Nucleotide sequence of a geminivirus associated with tomato leaf curl virus from India. Plant Dis 77:1168

    Article  Google Scholar 

  • Cohen S, Lapidot M (2007) Appearance and expansion of TYLCV: a historical point of view. In: Czosnek H (ed) Tomato yellow leaf curl virus disease: management, molecular biology, breeding for resistance. Springer, The Netherlands, pp 3–12

    Chapter  Google Scholar 

  • Czosnek H, Ber R, Antignus Y, Cohen S, Navot N, Zamir D (1988) Isolation of tomato yellow leaf curl virus, a geminivirus. Phytopathology 78:508–512

    Article  Google Scholar 

  • FAOSTAT (2013) Food and Agriculture Organization of the United Nations. http://faostat.fao.org. Accessed 10 Feb 2014

  • Fauquet CM, Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X (2008) Geminivirus strain demarcation and nomenclature. Arch Virol 153:783–821

    Article  CAS  PubMed  Google Scholar 

  • Giordano LB, Bezerra IC, Ferreira PTO, Borges Neto CR (1999) Breeding tomatoes for resistance to whitefly-transmitted geminivirus with bipartite genome in Brazil. Acta Hortic 487:357–360

    Google Scholar 

  • Hanson PM, Bernacchi D, Green S, Tanksley SD, Muniyappa V, Padmaja S, Chen HM, Kuo G, Fang D, Chen JT (2000) Mapping a wild tomato introgression associated with tomato yellow leaf curl virus resistance in a cultivated tomato line. J Am Soc Hortic Sci 125:15–20

    CAS  Google Scholar 

  • Hedrick UP, Booth NO (1908) Mendelian characters in tomato. Proc Am Soc Hortic Sci 5:19–24

    Google Scholar 

  • Hou Y-M, Paplomatas EJ, Gilbertson RL (1998) Host adaptation and replication properties of two bipartite geminiviruses and their pseudorecombinants. Mol Plant Microbe Interact 11:208–217

    Article  CAS  Google Scholar 

  • Hutton SF, Scott JW, Schuster DJ (2012) Recessive resistance to tomato yellow leaf curl virus from the tomato cultivar ‘Tyking’ is located in same region as ty-5 on chromosome 4. J Am Soc Hortic Sci 47(3):324–327

    Google Scholar 

  • Ji Y, Scott JW (2006) Ty-3, a begomovirus resistance locus linked to Ty-1 on chromosome 6 of tomato. Rep Tomato Genet Coop 56:22–25

    Google Scholar 

  • Ji Y, Schuster DJ, Scott JW (2007a) Ty-3, a begomovirus resistance locus near the Tomato yellow leaf curl virus resistance locus Ty-1 on chromosome 6 of tomato. Mol Breed 20:271–284

    Article  CAS  Google Scholar 

  • Ji Y, Scott JW, Hanson P, Graham E, Maxwell DP (2007b) Sources of resistance, inheritance, and location of genetic loci conferring resistance to members of the tomato-infecting begomoviruses. In: Czosnek H (ed) Tomato yellow leaf curl virus disease: management, molecular biology, breeding for resistance. Springer, The Netherlands, pp 343–362

    Chapter  Google Scholar 

  • Ji Y, Scott JW, Schuster DJ (2009a) Toward fine mapping of the tomato yellow leaf curl virus resistance gene Ty-2 on chromosome 11 of tomato. Hortic Sci 44(3):614–618

    Google Scholar 

  • Ji Y, Scott JW, Schuster DJ, Maxwell DP (2009b) Molecular mapping of Ty-4, a tomato yellow leaf curl virus resistance locus on chromosome 3 of tomato. J Am Soc Hortic Sci 134(2):281–288

    Google Scholar 

  • Jyothsna P, Haq QMI, Singh P, Sumiya KV, Praveen S, Rawat R, Briddon RW, Malathi VG (2013) Infection of tomato leaf curl New Delhi virus (ToLCNDV), a bipartite begomovirus with betasatellites, results in enhanced level of helper virus components and antagonistic interaction between DNA B and betasatellites. Appl Microbiol Biotechnol 97:5457–5471

    Article  CAS  PubMed  Google Scholar 

  • Kadirvel P, de la Pana R, Schaffeitner R, Huang S, Geethanjali S, Kenyon L, Tsai W, Hanson P (2013) Mapping of QTLs in tomato line FLA456 associated with resistance to a virus causing yellow leaf curl disease. Euphytica 190:297–308

    Article  CAS  Google Scholar 

  • Kalloo G, Banerjee MK (1990) Transfer of tomato leaf curl virus resistance from Lycopersicon hirsutum f. glabratum to L. esculentum. Plant Breed 105:156–159

    Article  Google Scholar 

  • Kings AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (2011) Virus taxonomy. Ninth report of the international committee on taxonomy of viruses. Elsevier/Academic Press, London/San Diego

    Google Scholar 

  • Lapidot M, Friedmann M (2002) Breeding for resistance to whitefly transmitted geminiviruses. Ann Appl Biol 140:109–127

    Article  Google Scholar 

  • Lapidot M, Ben-Joseph R, Cohen L, Machbash Z, Levy D (2006) Development of a scale for evaluation of tomato yellow leaf curl virus resistance level in tomato plants. Phytopathology 96:1404–1408

    Article  CAS  PubMed  Google Scholar 

  • Mejia L, Teni RE, Garcia BE, Fulladolsa AC, Mendez L (2010) Preliminary observations on the effectiveness of five introgressisons for resistance to begomoviruses in tomatoes. Rep Tomato Genet Coop 60:41–53

    Google Scholar 

  • Namrata J, Saritha RK, Datta D, Singh M, Dubey RS, Rai AB, Rai M (2010) Molecular characterisation of tomato leaf curl Palampur virus and Pepper leaf curl betasatellite naturally infecting Pumpkin (Cucurbita moschata) in India. Indian J Virol 21:128–132

    Article  PubMed Central  PubMed  Google Scholar 

  • Pilowsky M, Cohen S (1974) Inheritance of resistance to tomato yellow leaf curl virus in tomatoes. Phytopathology 64:632–635

    Article  Google Scholar 

  • Pilowsky M, Cohen S (1990) Tolerance to tomato yellow leaf curl virus derived from Lycopersicon peruvianum. Plant Dis 74:248–250

    Article  Google Scholar 

  • R Core Team (2012) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna. http://www.R-project.org/. Accessed 28 Jan 2014

  • Scott JW, Stevens MR, Barten JHM, Thome CR, Polston JE, Schuster DJ, Serra CA (1996) Introgression of resistance to whitefly-transmitted geminiviruses from Lycopersicon chilense to tomato. In: Gerling D, Mayer RT (eds) Bemisia 1995: taxonomy, Biology, Damage, Control and Management. Intercept, Andover, pp 357–377

    Google Scholar 

  • Tiwari N, Padmalatha KV, Singh VB, Haq QMI, Malathi VG (2010) Tomato leaf curl Banglaore virus (ToLCBV): infectivity and enhanced pathogenicity with diverse betasatellites. Arch Virol 155:1343–1347

    Article  CAS  PubMed  Google Scholar 

  • Vasudeva RS, Samraj J (1948) A leaf curl disease of tomato. Phytopathology 38:364–369

    Google Scholar 

  • Venkataravanappa V, Reddy LL, Swarnalatha P, Mahesh B, Rai AB, Reddy K (2014) Association of Tomato leaf curl Joydebpur virus and a betasatellite with leaf curl disease of eggplant. Phytoparasitica 42:109–120

    Article  Google Scholar 

  • Verlaan MG, Hutton SF, Ibrahem RM, Kormelink R, Visser RGF, Scott JW, Edwards JD, Bai Y (2013) The tomato yellow leaf curl virus resistance genes Ty-1 and Ty-3 are allelic and code for DFDGD-class RNA–dependent RNA Polymerases. PLoS Genet 9(3):e1003399

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Vidavski F, Czosnek H, Gazit S, Levy D, Lapidot M (2008) Pyramiding of genes conferring resistance to tomato yellow leaf curl virus from different wild tomato species. Plant Breed 127:625–631

    Article  Google Scholar 

  • Yang X, Caro M, Hutton SF, Scott JW, Guo Y, Wang X, Rashid MH, Szinay D, de Jong H, Visser RG, Bai Y, Du Y (2014) Fine mapping of the tomato yellow leaf curl virus resistance gene Ty-2 on chromosome 11 of tomato. Mol Breed. doi:10.1007/s11032-014-0072-9

    PubMed Central  PubMed  Google Scholar 

  • Zamir D, Ekstein-Michelson I, Zakay Y, Navot N, Zeidan M, Sarfatti M, Eshed Y, Harel E, Pleban T, van-Oss H, Kedar N, Rabinowitch HD, Czosnek H (1994) Mapping and introgression of a tomato yellow leaf curl virus tolerance gene, Ty-1. Theor Appl Genet 88:141–146

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

We are grateful to Dr. TA More, Dr. PS Naik and Dr. M Singh for providing necessary infrastructural support. We thank Dr. Peter Hanson, World Vegetable Center for generously providing seeds of F2 base population. We acknowledge the excellent field and greenhouse assistance provided by Sh Rajaram. This study was undertaken as a part of in-house project on ‘Breeding resistance to tomato leaf curl virus disease’ funded by Indian Council of Agricultural Research (ICAR), New Delhi.

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Authors and Affiliations

  1. Indian Institute of Vegetable Research, Post Bag No. 1, P. O. Jakhini, Shahanshapur, Varanasi, UP, 221305, India

    H. C. Prasanna, Sarvesh P. Kashyap, Ram Krishna, D. P. Sinha & Suresh Reddy

  2. Division of Plant Pathology, Advanced Centre for Plant Virology, Indian Agriculture Research Institute, Pusa Camus, New Delhi, 110012, India

    V. G. Malathi

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  1. H. C. Prasanna
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  2. Sarvesh P. Kashyap
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  3. Ram Krishna
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  4. D. P. Sinha
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  5. Suresh Reddy
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  6. V. G. Malathi
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Correspondence to H. C. Prasanna.

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Prasanna, H.C., Kashyap, S.P., Krishna, R. et al. Marker assisted selection of Ty-2 and Ty-3 carrying tomato lines and their implications in breeding tomato leaf curl disease resistant hybrids. Euphytica 204, 407–418 (2015). https://doi.org/10.1007/s10681-015-1357-8

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