Abstract
Cabbage Fusarium wilt (CFW), caused by Fusarium oxysporum f. sp. conglutinans, is one of the most devastating diseases of cabbage worldwide. In recent years, CFW has been spreading across northern Chinad. Thus, development of resistant cultivars is necessary to control CFW. Here we report microspore culture from a cross of two elite cabbage inbred lines, 96–100 (highly resistant to CFW) and 01–20 (highly susceptible to CFW), in order to obtain doubled haploids (DHs) to cultivate cabbage materials for resistance breeding. A total of 196 DHs were obtained in 2011 and 2012 and three elite DH lines were selected from them through agronomic trait evaluation as well as marker-assisted selection. These three elite DH lines were crossed with elite cabbage inbred lines to create hybrids, which were then evaluated for their resistance and agronomic traits. Three excellent hybrids were selected. The use of microspore culture and marker-assisted selection greatly shortened the time required to obtain these excellent hybrids. These resistant varieties may become an effective control of CFW in diseased areas.
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Acknowledgments
This work was financially supported by grants from the National High Technology Research and Development Program of China (863 Program, 2012AA100101, 2012AA100102, 2012AA100103), the Key Projects in the National Science & Technology Pillar Program during the Twelfth Five-Year Plan Period (2012BAD02B01, 2013BAD01B04), the Earmarked Fund for the Modern Agro-Industry Technology Research System, China (nycytx-35-gw01), the Major State Basic Research Development Program (973 Program, 2012CB113906) and the National Natural Science Foundation of China (31171958, 31272180, 31272003). The work reported here was carried out in the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Beijing 100081, China.
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Lv, Hh., Wang, Qb., Yang, Lm. et al. Breeding of cabbage (Brassica oleracea L. var. capitata) with fusarium wilt resistance based on microspore culture and marker-assisted selection. Euphytica 200, 465–473 (2014). https://doi.org/10.1007/s10681-014-1197-y
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DOI: https://doi.org/10.1007/s10681-014-1197-y