Abstract
Salinity is globally a major constraint for crop production. Breeding for salinity tolerance is an effective approach to improve crop production and productivity under saline conditions provided it is based on a good understanding of the genetic control of salinity tolerance. This study deals with mapping QTLs for salinity tolerance in durum wheat (Triticum durum) by association analysis using SSR markers. A total of 119 varieties were treated in 100 mM of NaCl solution and the salinity tolerance indices (STI) for several traits were calculated as parameters to assess salinity tolerance. Among the traits assessed, the increased proportion of dead leaves (%DL) was the most suitable parameter for assessment of salinity tolerance in durum varieties at early vegetative stages because of a broader range of variation among varieties and narrower range of variation within varieties compared to other traits. The QTL associated with salinity tolerance using %DL as a parameter was detected on chromosome 4B. An additional 11 QTLs associated with seven parameters using STI of other traits were detected on chromosomes 3A, 5A, 5B, 6A and 7A.
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Abbreviations
- QTL:
-
Quantitative trait locus
- SSR:
-
Simple sequence repeats
- STI:
-
Salt tolerance index
- EC:
-
Electrical conductivity
- LD:
-
Linkage disequilibrium
- GLM:
-
General linear model
- MLM:
-
Mixed linear model
- CHL:
-
Chlorophyll content
- NT:
-
Number of tillers per plant
- NL:
-
Number of leaves per tiller
- LL:
-
Leaf length
- TFW:
-
Total fresh weight of shoot and root
- SL:
-
Shoot length
- RL:
-
Root length
- SDW:
-
Shoot dry weight
- RDW:
-
Root dry weight
- %DL:
-
Increased percentage of dead leaves
- NFS:
-
Number of fertile spikes
- PH:
-
Plant height
- BIO:
-
Biomass production
- NS:
-
Number of seeds per spike
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Acknowledgments
This research was carried out by GRANDE project supported by JST/JICA, SATREPS (Science and Technology Research Partnership for Sustainable Development), Japan.
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Turki, N., Shehzad, T., Harrabi, M. et al. Detection of QTLs associated with salinity tolerance in durum wheat based on association analysis. Euphytica 201, 29–41 (2015). https://doi.org/10.1007/s10681-014-1164-7
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DOI: https://doi.org/10.1007/s10681-014-1164-7