Abstract
Generating segregating populations and ‘pure lines’ is an essential component in many projects of plant breeding and biological studies. Time taken to obtain these materials is often a critical factor restricting the progress of such projects. We report a procedure here which, by combining embryo culture with managements of watering regimes, lighting intensity and duration, temperature and quantity of potting mixture, allows the production of up to eight generations of wheat and nine generations of barley per annum. By dramatically shortening the time frame required to obtain segregating populations and ‘pure lines’, the procedure could find wide applications in breeding and biological studies.
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References
Baus AD, Franzmann L, Meinke DW (1986) Growth in vitro of arrested embryos from lethal mutants of Arabidopsis thaliana. Theor Appl Genet 72:577–586
Chavez R, Brown CR, Iwanaga M (1988) Application of interspecific sesquiploidy to introgression of PLRV resistance from non-tuber-bearing Solanum etuberosum to cultivated potato germplasm. Theor Appl Genet 76:497–500
Chen GD, Li HB, Zheng Z, Wei YM, Zheng YL, McIntyre CL, Zhou MX, Liu CJ (2012) Characterization of a QTL affecting spike morphology on the long arm of chromosome 3H in barley (Hordeum vulgare L.) based on near isogenic lines and a NIL-derived population. Theor Appl Genet 125:1385–1392
Gerjets T, Scholefield D, Foulkes MJ, Lenton JR, Holdsworth MJ (2010) An analysis of dormancy, ABA responsiveness, after-ripening and pre-harvest sprouting in hexaploid wheat (Triticum aestivum L.) caryopses. J Exp Bot 61:597–607
Krauter R, Steinmetz A, Friedt W (1991) Efficient interspecific hybridization in the genum Helianthus via ‘embryo rescue’ and characterization of the hybrids. Theor Appl Genet 82:521–525
Kuboyama T, Shintaku Y, Takeda G (1991) Hybrid plant of Phaseolus vulgaris L. and P. lunatus L. obtained by means of embryo rescue and confirmed by restriction endonuclease analysis of rDNA. Euphytica 54:177–182
Laurie DA, Reymondie S (1991) High frequencies of fertilization and haploid seedling production in crosses between commercial hexaploid wheat varieties and maize. Plant Breed 106:182–189
Li HB, Xie GQ, Ma J, Liu GR, Wen SM, Ban T, Chakraborty S, Liu CJ (2010) Genetic relationships between resistances to Fusarium head blight and crown rot in bread wheat (Triticum aestivum L.). Theor Appl Genet 121:941–950
Ma J, Li HB, Zhang CY, Yang XM, Liu YX, Yan GJ, Liu CJ (2010) Identification and validation of a major QTL conferring crown rot resistance in hexaploid wheat. Theor Appl Genet 120:1119–1128
Ma J, Yan GJ, Liu CJ (2011) Development of near-isogenic lines for a major QTL on 3BL conferring Fusarium crown rot resistance in hexaploid wheat. Euphytica 183:147–152
Munoz CE, Lyrene PM (1985) In vitro attempts to overcome the cross-incompatibility between Vaccinium corymbosum L. and V. elliottii Chapm. Theor Appl Genet 69:591–596
O’Donoughue LS, Bennett MD (1994) Durum wheat haploid production using maize wide-crossing. Theor Appl Genet 89:559–566
Ortiz R, Tretowan R, Ortiz Ferrara GO, Iwanaga M, Dodds JH, Crouch JH, Crossa J, Braun H-J (2007) High yield potential, shuttle breeding, genetic diversity, and a new international wheat improvement strategy. Euphytica 157:365–384
Plourde A, Comeau A, Fedak G, St-Pierre CA (1989) Production and cytogenetics of hybrids of Triticum aestivum × Leymus innovatus. Theor Appl Genet 78:436–444
Rogers SO, Quatrano RS (1983) Morphological staging of wheat caryopsis development. Am J Bot 70:308–311
Sharma HC, Ohm HW (1990) Crossability and embryo rescue enhancement in wide crosses between wheat and three Agropyron species. Euphytica 49:209–214
Wan Y, Poole RL, Huttly AK, Toscano-Underwood C, Feeney K, Welham S, Gooding MJ, Mills C, Edwards KJ, Shewry PR, Mitchell RAC (2008) Transcriptome analysis of grain development in hexaploid wheat. BMC Genomics 9:121. doi:10.1186/1471-2164-9-121
Xu J, Kasha KJ (1992) Transfer of a dominant gene for powdery mildew resistance and DNA from Hordeum bulbosum into cultivated barley (H. vulgare). Theor Appl Genet 84:771–777
Acknowledgments
The authors are grateful to Dr John Manners who encouraged us to publish this procedure which has been routinely used in our labs. Z. Zheng would like to thank the University of Western Australia and China Scholarship Council (CSC) for his PhD scholarships, and GD Chen is grateful to CSC and Sichuan Agricultural University for supporting his visit to CSIRO.
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Zheng, Z., Wang, H.B., Chen, G.D. et al. A procedure allowing up to eight generations of wheat and nine generations of barley per annum. Euphytica 191, 311–316 (2013). https://doi.org/10.1007/s10681-013-0909-z
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DOI: https://doi.org/10.1007/s10681-013-0909-z