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Intergeneric hybrid between Chrysanthemum × morifolium and Artemisia japonica achieved via embryo rescue shows salt tolerance

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Abstract

Using embryo rescue, we generated an intergeneric hybrid between Chrysanthemum × morifolium ‘Maoyan’ and Artemisia japonica Thunb. Cytological tests confirmed that regenerated plantlets were all genuine hybrids possessing 45 chromosomes, with 27 chromosomes inherited from C. × morifolium (2n = 6x = 54) and the other 18 derived from A. japonica (2n = 4x = 36). Hybrid plant flowered normally. The shape and color of the hybrid flowers and leaves resembled those of chrysanthemum, while leaf width, leaf length, plant height, and inflorescence diameter were intermediate between those of the parents. Hybrid plant had higher levels of chlorophyll and free proline, and lower concentrations of malondialdehyde and Na+, than the maternal parent (C. × morifolium), and these levels were correlated with the hybrid’s enhanced salt tolerance. These results clearly demonstrate that intergeneric hybridization is an effective method of cultivar improvement in chrysanthemum.

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Acknowledgments

This work was supported by the Fundamental Research Funds for the Central Universities (KYJ 200907), 863 project of Ministry of Science and Technology of the People’s Republic of China (2011AA100208), Fund for Independent Innovation of Agricultural Sciences in Jiangsu Province (CX(12)2020), the Science and Technology Program of Jiangsu Province (BE2011325, BE2012350), the Program for New Century Excellent Talents in University of Chinese Ministry of Education (Grant No. NCET-10-0492), and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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  1. College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

    Wen-ying Zhu, Jia-fu Jiang, Su-mei Chen, Liang Wang, Li-li Xu, Hai-bin Wang, Pei-ling Li, Zhi-yong Guan & Fa-di Chen

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  1. Wen-ying Zhu
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  2. Jia-fu Jiang
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  4. Liang Wang
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  8. Zhi-yong Guan
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  9. Fa-di Chen
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Correspondence to Fa-di Chen.

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Zhu, Wy., Jiang, Jf., Chen, Sm. et al. Intergeneric hybrid between Chrysanthemum × morifolium and Artemisia japonica achieved via embryo rescue shows salt tolerance. Euphytica 191, 109–119 (2013). https://doi.org/10.1007/s10681-013-0869-3

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