Abstract
A cassava F1 population raised from the cross SC6 × Mianbao was used to construct a genetic linkage map. The map incorporated 200 polymorphic amplified fragment length polymorphism, sequence-related amplified polymorphism, simple sequence repeat (SSR), and expressed sequence tag (EST)–SSR markers which fit a 1:1 segregation ratio. It comprised 20 linkage groups (LGs) and spanned a genetic distance of 1645.1 cM with an average marker interval of 8.2 cM. Fifty-seven repeatedly detected QTLs (rd-QTLs) for three phenotypic traits (fresh root yield, root dry matter content, and root starch content) were identified in the F1 population in four trials of year 2003, 2004, 2005, and 2008 by inclusive composite interval mapping. Among the 57 rd-QTLs, 25 rd-QTLs were linked to SSR/EST–SSR markers, which will help to facilitate marker-assisted selective breeding in cassava, and 15 marker intervals on ten LGs showed pleiotropic effects.
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References
Akano AO, Dixon AGO, Mba C, Barrera E, Fregene M (2002) Genetic mapping of a dominant gene conferring resistance to cassava mosaic disease. Theor Appl Genet 105:521–525
Akinbo O, Gedi M, Ekpo EJA, Oladele J, Dixon AGO (2007) Detection of RAPD markers-linked to resistance to cassava anthracnose disease. Afr J Biotech 6(6):677–682
Balyejusa Kizito E, Rönnberg-Wästljung AC, Egwang T, Gullberg U, Fregene M, Westerbergh A (2007) Quantitative trait loci controlling cyanogenic glucoside and dry matter content in cassava (Manihot esculenta Crantz) roots. Hereditas 144(4):129–136
Chen X, Xia ZQ, Fu YH, Lu C, Wang WQ (2010) Constructing a genetic linkage map using an F1 population of non-inbred parents in cassava (Manihot esculenta Crantz). Plant Mol Biol Rep 28:676–683
Cortés DF, Reilly K, Okogbenin E, Beeching JR, Iglesias C, Tohme J (2002) Mapping wound-response genes involved in post-harvest physiological deterioration (PPD) of cassava (Manihot esculenta Crantz). Euphytica 128:47–53
Fregene M, Angel F, Gomez R, Rodriguez F, Chavarriaga P, Roca W, Tohme J, Bonierbale M (1997) A molecular genetic map of cassava (Manihot esculenta Crantz). Theor Appl Genet 95:431–441
Hershey CH, Jennings DL (1992) Progress in breeding cassava for adaptation to stress. Plant Breed Abstr 62(8):823–831
Jorge V, Fregene M, Duque MC, Bonierbale MW, Tohme J, Verdier V (2000) Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz). Theor Appl Genet 101:865–872
Jorge V, Fregene M, Vélez CM, Duque MC, Tohme J, Verdier V (2001) QTL analysis of field resistance to Xanthomonas axonopodis pv. manihotis in cassava. Theor Appl Genet 102:564–571
Kunkeaw S, Tangphatsornruang S, Smith DR, Triwitayakorn K (2010) Genetic linkage map of cassava (Manihot esculenta Crantz) based on AFLP and SSR markers. Plant Breed 129:112–115
Li G, Quiros CF (2001) Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet 103:455–461
Li HH, Ye GY, Wang JK (2007) A modified algorithm for the improvement of composite interval mapping. Genetics 175:361–374
Lokko Y, Danquah EY, Offei SK, Dixon AGO, Gedil MA (2005) Molecular markers associated with a new source of resistance to the cassava mosaic disease. Afr J Biotech 4(9):873–881
Lopez CE, Quesada-Ocampo LM, Bohorquez A, Duque MC, Vargas J, Joe T, Verdier V (2007) Mapping EST-derived SSRs and ESTs involved in resistance to bacterial blight in Manihot esculenta. Genome 50:1078–1088
Mitchell GA (1990) Methods of starch analysis. Starch/Starke 42:132–134
Okogbenin E, Fregene M (2002) Genetic analysis and QTL mapping of early root bulking in an F1 population of non-inbred parents in cassava (Manihot esculenta Crantz). Theor Appl Genet 106:58–66
Okogbenin E, Marin J, Fregene M (2006) An SSR-based molecular genetic map of cassava. Euphytica 147(3):433–440
Okogbenin E, Marin J, Fregene M (2008) QTL analysis for early yield in a pseudo F2 population of cassava. Afr J Biotech 7(2):131–138
Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: JoinMap. Plant J 5:739–744
Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Friters A, Pot J, Paleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23(21):4407–4414
Wydra K, Zinsou V, Jorge V, Verdier V (2004) Identification of pathotypes of Xanthomonas axonopodis pv. manihotis in Africa and detection of quantitative trait loci and markers for resistance to bacterial blight of cassava. Phytopathology 94:1084–1093
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This work was supported financially by the National Basic Research and Development Program (2010CB126600) and the earmarked fund for China Agriculture Research System (CARS-12). We are grateful to Martin Fregene (the Director of Biocassava Plus at the Donald Danforth Plant Science Center, formerly of CIAT) for his gift of the genomic SSR primer pairs.
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Xin Chen and Yuhua Fu are co-first author.
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Chen, X., Fu, Y., Xia, Z. et al. Analysis of QTL for yield-related traits in cassava using an F1 population from non-inbred parents. Euphytica 187, 227–234 (2012). https://doi.org/10.1007/s10681-012-0662-8
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DOI: https://doi.org/10.1007/s10681-012-0662-8