Abstract
The efficiency of using monosomic alien addition lines (MAALs) to introgress agronomical traits of interest carried by wild diploid Gossypium species into the main cultivated cotton species G. hirsutum depends on the opportunities of confronting the alien chromosome with the recipient background genome at each generation and on the occurrence of translocations and homoeologous recombinations. The selfed-progeny of five MAALs of G. australe in G. hirsutum was screened with SSR markers to determine the transmission frequency of the alien chromosome and monitor its integrity. Three MAALs revealed a transmission frequency significantly lower than the expected ratio and one MAAL presented an exclusive transmission of the additional chromosome. In these four MAAL the alien chromosome was transmitted almost unaltered. With the fifth MAAL the alien chromosome was normally transmitted but was altered in half of the plants carrying it. In one MAAL, normally carrying brown fiber, the emergence of some plants carrying white and brown fiber revealed the somatic elimination of the additional chromosome. The loss of this chromosome seems to be triggered by its deletion.
Similar content being viewed by others
References
Ahoton L, Lacape JM, Baudoin JP, Mergeai G (2003) Introduction of Australian diploid cotton genetic variation into upland cotton. Crop Sci 43:1999–2005
Becerra Lopez-Lavalle LA, Brubaker CL (2007) Frequency and fidelity of alien chromosome transmission in Gossypium hexaploid bridging populations. Genome 50:479–491
Benbouza H, Baudoin JP, Mergeai G (2006a) Amélioration de la méthode d’extraction d’ADN au CTAB appliqué aux feuilles de cotonnier. Biotechnol Agron Soc Environ 10:73–76
Benbouza H, Jacquemin JM, Baudoin JP, Mergeai G (2006b) Optimization of a reliable, fast, cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels. Biotechnol Agron Soc Environ 10:77–81
Brubaker CL, Brown AHD (2003) The use of multiple alien chromosome addition aneuploids facilitates genetic linkage mapping of the Gossypium G genome. Genome 46:774–791
Brubaker CL, Brown AHD, Stewart JMcD, Kilby MJ, Grace JP (1999) Production of fertile hybrid germplasm with diploid Australian Gossypium species for cotton improvement. Euphytica 108:199–213
Cameron DR, Moav R (1957) Inheritance in Nicotiana tabacum XXVII. Pollen killer, an alien genetic locus inducing abortion of microspores not carrying it. Genetics 42:326–335
Chen PD, Tsujimoto H, Gill BS (1994) Transfer of genes promoting homeologous pairing from Triticum speltoides to common wheat. Theor Appl Genet 88:97–101
Gao D, Jung C (2002) Monosomic addition lines of Beta corolliflora in sugar beet: plant morphology and leaf spot resistance. Plant Breeding 121:81–86
Gernand D, Rutten T, Varshney A, Rubtsova M, Prodanovic S, Brüß C, Kumlehn J, Matzk F, Houben A (2005) Uniparental chromosome elimination at mitosis and interphase in wheat and pearl millet crosses involves micronucleus formation, progressive heterochromatinization, and DNA fragmentation. Plant Cell 17:2431–2438
Gupta SB (1968) The unstable behavior of a chromosomal fragment of Nicotiana plumbaginifolia responsible for chlorophyll variegation in N. tabacum. Genetics 59:453–463
Hau B (1981) Lignées d’addition sur Gossypium hirsutum L. I. Utilisation de l’hybridation interspécifique et de la méthode des lignées d’addition pour l’amélioration du cotonnier. Cot Fib Trop 26:247–258
Jiang J, Friebe B, Gill BS (1994) Recent advances in alien gene transfer in wheat. Euphytica 73:199–212
Koba T, Handa T, Shimada T (1991) Efficient production of wheat-barley hybrids and preferential elimination of barley chromosomes. Theor Appl Genet 81:285–292
Konan O, Baudoin JP, D’Hont A, Mergeai G (2009) Bridging classical and molecular cytogenetics of Gossypium. In: Paterson AH (ed) Genetics and genomics of cotton. Springer, New York, pp 257–281
Kynast RG, Riera-Lizarazu O, Vales MI, Okagaki RJ, Maquieira SB, Chen G, Ananiev EV, Odland WE, Russell CD, Stec AO, Livingston SM, Zaia HA, Rines HW, Phillips RL (2001) A complete set of maize individual chromosome additions to the oat genome. Plant Physiol 125:1216–1227
Lapitan NLV, Sears RG, Gill BS (1984) Translocations and other karyotypic structural changes in wheat × rye hybrids regenerated from tissue culture. Theor Appl Genet 68:547–554
Laurie DA, Bennett MD (1989) The timing of chromosome elimination in hexaploid wheat × maize crosses. Genome 32:953–961
Maan SS (1975) Exclusive preferential transmission of an alien chromosome in common wheat. Crop Sci 15:287–292
Maguire MP (1963) High transmission frequency of a Tripsacum chromosome in corn. Genetics 48:1185–1194
Masoudi-Nedjad A, Nasuda S, McIntosh RA, Endo TR (2002) Transfer of rye chromosome segments to wheat by a gametocidal system. Chrom Res 10:349–357
Masoudi-Nedjad A, Nasuda S, Bihoreau MT, Waugh R, Endo TR (2005) An alternative to radiation hybrid mapping for large-scale genome analysis in barley. Mol Gen Genomics 274:589–594
McClintock B (1941) The stability of broken ends of chromosomes in Zea mays. Genetics 26:234–282
Mergeai G (1992) Utilisation du cotonnier sauvage Gossypium areysianum Defl. Hutch. Pour l’amélioration de l’espèce cultivée Gossypium hirsutum L. PhD Thesis, Faculté des Sciences Agronomiques de Gembloux
Mergeai G (2006) Introgressions interspécifiques chez le cotonnier. Cah Agric 15:135–143
Miller TE, Hutchinson J, Chapman V (1982) Investigation of a preferentially transmitted Aegilops sharonensis chromosome in wheat. Theor Appl Genet 61:27–33
Moav R (1961) Genetic instability in Nicotiana hybrids II. Studies of the Ws (pbg) locus in N. plumbaginifolia in N. tabacum nuclei. Genetics 46:1069–1087
Nasuda S, Friebe B, Gill BS (1998) Gametocidal genes induce chromosome breakage in the interphase prior to the first mitotic cell division of the male gametophyte in wheat. Genetics 149:1115–1124
Poisson C (1970) Contribution à l’étude de l’hybridation interspécifique dans le genre Gossypium : transfert de matériel génétique de l’espèce diploïde sauvage G. anomalum à l’espèce cultivée G. hirsutum. PhD Thesis, Université d’Orsay
Rhoades MM (1942) Preferential segregation in maize. Genetics 27:395–407
Riera-Lizarazu O, Rines HW, Philips RP (1996) Cytological and molecular characterization of oat × maize partial hybrids. Theor Appl Genet 93:123–135
Rooney WL, Stelly DM (1991) Preferential transmission and somatic elimination of a Gossypium sturtianum chromosome in G. hirsutum. Crop Sci 82:151–155
Rooney WL, Stelly DM, Altman DW (1991) Identification of four Gossypium sturtianum monosomic alien addition derivatives from a backcrossing program with G.hirsutum. Crop Sci 31:337–341
Sarr D, Lacape JM, Jacquemin JM, Toussaint A, Palm R, Ahoton L, Baudoin JP, Mergeai G (2011) Isolation of five new monosomic alien addition lines of Gossypium australe in G. hirsutum by SSR and GISH analyses. Plant Breed 130:60–66
Sears ER (1993) Use of radiation to transfer alien chromosome segments to wheat. Crop Sci 33:897–901
Shi F, Endo TR (1999) Genetic induction of structural changes in barley chromosomes added to common wheat by a gametocidal chromosome derived from Aegilops cylindrical. Genes Genet Syst 74:49–54
Shi F, Endo TR (2000) Genetic induction of chromosomal rearrangements in barley chromosome 7H added to common wheat. Chromosoma 109:358–363
Stewart JMcD (1995) Potential for crop improvement with exotic germplasm and genetic engineering. In: Constable GA, Forrester NW (eds) Challenging the future: proceedings of the World Cotton Research conference-1. CSIRO, Melbourne, pp 313–327
Walters MS (1950) Spontaneous breakage and reunion of meiotic chromosomes in the hybrid Bromus trinii × B. maritimus. Genetics 35:11–37
Wendel JF, Brubaker CL, Percival AE (1992) Genetic diversity in Gossypium hirsutum and the origin of upland cotton. Am J Bot 79:1291–1310
Acknowledgment
We thank the Belgian Technical Cooperation for funding the PhD scholarships of the first author.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Sarr, D., Lacape, JM., Jacquemin, JM. et al. Alien chromosome transmission and somatic elimination in monosomic addition lines of Gossypium australe F. Muell in G. hirsutum L.. Euphytica 183, 55–64 (2012). https://doi.org/10.1007/s10681-011-0479-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10681-011-0479-x