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What can the Viviparous-1 gene tell us about wheat pre-harvest sprouting?

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Abstract

Pre-harvest sprouting (PHS) of wheat greatly reduces the quality and economic value of grain, and PHS tolerance is one of the most important traits in wheat breeding. Two new Vp-1B alleles were identified in wheat genotypes with distinct PHS tolerance and ABA responsiveness and designated as Vp-1Bb and Vp-1Bc. These alleles had an insertion of 193-bp and a deletion of 83-bp in the third intron in the region of B3 domain, respectively. Further analysis indicated that different Vp-1B alleles showed different expression and responsiveness to ABA exposure at transcriptional level, implying that they may regulate PHS tolerance. Based on these alleles, a STS marker Vp1B3 was developed and validated among a set of 89 Chinese wheat accessions. The results indicated that Vp1B3 may be useful as an efficient and reliable co-dominant marker in the evaluation of wheat germplasm for PHS tolerance and marker-assisted breeding for PHS tolerant cultivars. Moreover, analysis of a collection of 490 EU winter wheat varieties revealed not only the prevalence of Vp-1B alleles but also a new allele Vp-1Bd which does not occur in Chinese wheats.

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Abbreviations

PHS:

Pre-harvest sprouting

Vp1 :

Viviparous-1

RL:

Recommended list

NL:

National list

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Acknowledgments

This project was partly funded by the National High-Tech Research Program (2006AA10Z115) and the National Basic Research Program (2009CB118300). Rothamsted Research receives grant-aided support from the Biotechnology and Biological Sciences Research Council (BBSRC) of the United Kingdom.

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Authors and Affiliations

  1. Institute of Crop Science/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China

    L. Q. Xia, Y. Yang, Y. Z. Ma, X. M. Chen & Z. H. He

  2. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466, Gatersleben, Germany

    M. S. Röder

  3. Crop Performance Improvement Division, Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK

    H. D. Jones & P. R. Shewry

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  1. L. Q. Xia
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  2. Y. Yang
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  3. Y. Z. Ma
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  4. X. M. Chen
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  5. Z. H. He
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  6. M. S. Röder
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  7. H. D. Jones
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  8. P. R. Shewry
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Correspondence to L. Q. Xia or P. R. Shewry.

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Xia, L.Q., Yang, Y., Ma, Y.Z. et al. What can the Viviparous-1 gene tell us about wheat pre-harvest sprouting?. Euphytica 168, 385–394 (2009). https://doi.org/10.1007/s10681-009-9928-1

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  • DOI: https://doi.org/10.1007/s10681-009-9928-1

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