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Molecular mapping of a major QTL conferring resistance to SCMV based on immortal RIL population in maize

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Abstract

Sugarcane mosaic virus (SCMV) is one of devastating pathogens in maize (Zea mays L.), and causes serious yield loss in susceptible cultivars. An effective solution to control the virus is utilizing resistant genes to improve the resistance of susceptible materials, whereas the basic work is to analyze the genetic basis of resistance. In this study, maize inbred lines Huangzao4 (resistant) and Mo17 (susceptible) were used to establish an F9 immortal recombinant inbred line (RIL) population containing 239 RILs. Based on this segregation population, a genetic map was constructed with 100 simple sequence repeat (SSR) markers selected from 370 markers, and it covers 1421.5 cM of genetic distance on ten chromosomes, with an average interval length of 14.2 cM. Analysis of the genetic map and resistance by mapping software indicated that a major quantitative trait locus (QTL) was between bin6.00 and bin6.01 on chromosome 6, linked with marker Bnlg1600 (0.1 cM of interval). This QTL could account for 50.0% of phenotypic variation, and could decrease 27.9% of disease index.

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Acknowledgments

This work was financially assisted by Sichuan Key Subject Program (SZD0420). We are grateful to Dr. Jun Yang (College of Life Sciences, China West Normal University, P. R. China) for critical reading of this manuscript.

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Authors and Affiliations

  1. College of Life Sciences, China West Normal University, Nanchong, 637002, China

    Xiao-Hong Liu & Zhen-Bo Tan

  2. Maize Research Institute, Sichuan Agricultural University, Ya’an, 625014, China

    Ting-Zhao Rong

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  1. Xiao-Hong Liu
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  2. Zhen-Bo Tan
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  3. Ting-Zhao Rong
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Correspondence to Xiao-Hong Liu.

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Liu, XH., Tan, ZB. & Rong, TZ. Molecular mapping of a major QTL conferring resistance to SCMV based on immortal RIL population in maize. Euphytica 167, 229–235 (2009). https://doi.org/10.1007/s10681-008-9874-3

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  • DOI: https://doi.org/10.1007/s10681-008-9874-3

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