Abstract
Screening toxicity tests are an effective method to characterize aluminum tolerant plants. In this paper, three screening procedures were used to assess aluminum tolerance among 13 cultivars of alfalfa (Medicago sativa L.). The procedures used were: Petri dish screening, black glass plot method and the soil-on-agar procedure. Major biological indices used to analyze aluminum tolerance were relative root length (RRL), relative germination rate (RGR), relative hypocotyl length (RHL), relative fresh weight (RFW) and root emergence (RE). Aluminum negatively affected all five indices. A Pearson correlation test indicated that the results of the three screening procedures were consistent, suggesting that all three could be used for screening purposes. However, because of the short test period and the simplicity of operation, the black glass plot method might be the best choice. Overall, cv. Super No. 7 and WL-525HQ were the most aluminum-tolerant alfalfa cultivars, while Pondus S was the most sensitive.
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Baluška F, Cvrčková F, Jones J, Volkmann D (2001) Sink plasmodesmata as gateways for phloem unloading, Myosin VIII and Calreticulin as molecular determinants of sink strength? Plant Physiol 126:39–46. doi:10.1104/pp.126.1.39
Bouton JH (1996) Screening the alfalfa core collection for acid soil tolerance. Crop Sci 36:198–200
Bouton JH, Radclinffe DE (1989) Effects of acid soil selection on agronomically important traits in alfalfa. In: Proceedings of XVI international grassland congress, Nice, France, 4–11 October 1989. Curle Printing Co., Inc., Minneapolis, pp 377–378
Bouton JH, Summer ME (1983) Alfalfa, Medicago sativa L, in highly weathered soils. V, Field performance of alfalfa selected for acid tolerance. Plant Soil 74:431–436. doi:10.1007/BF02181360
Buss GR, Lutz JA Jr, Hawkins GW (1975) Yield response of alfalfa cultivars and clones to several pH levels in Tatum subsoil. Agron J 67:331–334
Campbell TA, Elgin JH, Foy CD, Mc JE, Murtrey VIII (1988) Selection in alfalfa for tolerance to toxic levels of aluminum. Can J Plant Sci 68:743–753
Cao HF, Gao JX, Shu JM (1992) Study on the response of Pinus massoniana seedling to aluminium. Acta Ecol Sin 12:239–246
Clarkson DT (1965) The effect of aluminium and some other trivalent metal cations on cell division in the root apices of Allium cepa. Ann Bot (Lond) 29:309–315
Delhaize E, Ryan PR (1995) Aluminum toxicity and tolerance in plants. Plant Physiol 107:315–321
Delhaize E, Stuart C, Colin DB, Robin JB, Vidya CJ, Peter JR (1993) Aluminum tolerance in wheat (Triticum aestivum L.) (I. Uptake and distribution of aluminum in root apices). Plant Physiol 103:685–693
Delhaize E, Ryan PR, Hebb DM, Yamamoto Y, Sasaki T, Matsumoto HI (2004) Engineering high-level aluminum tolerance in barley with the ALMT1 gene. Proc Natl Acad Sci USA 101:15249–15254. doi:10.1073/pnas.0406258101
Foy CD (1976) General principles involved in screening plants for aluminum and manganese tolerance. In: Wright MJ (ed) Plant adaptation to mineral stress in problem soils. New York State College of Agriculture and Life Science. Cornell University, Ithaca, pp 255–267
Foy CD (1984) Physiological effects of hydrogen, aluminum, and manganese toxicity in acid soil. In: Adams F (ed) Soil acidity and liming, Agronomy no. 12, 2nd edn. Am Soc Agron, Madison, pp 57–98
Foy CD (1988) Plant adaptation to acid, aluminum toxic soils. Commun Soil Sci Plant Anal 19:959–987
Fuente de la JM, Verenice RR, José LCP, Luis HE (1997) Aluminum tolerance in transgenic plants by alteration of citrate synthesis. Science 276:1566–1568. doi:10.1126/science.276.5318.1566
Gong XH, Gao SX (2003) Cd-error by adsorption of filter paper. J Guizhou Univ (Natural Science) 20:314–315
Hang A (1984) Molecular aspects of aluminum toxicity. CRC Crit Rev Plant Genet 1(4):345–373
Jones DL, Kochain LV (1995) Aluminum inhibition of the inositol 1, 4, 5-Triphosphate signal tranduction pathway in wheat roots: a role in aluminum toxicity? Plant Cell 7:1913–1922
Hartel WA, Bouton JH (1989) Rhizobium meliloti inoculation of alfalfa selected for tolerance to acid, aluminum-rich soils. Plant Soil 116:283–285. doi:10.1007/BF02214560
Kollmeier M, Felle HH, Horst WJ (2000) Genotypical differences in aluminum resistance of maize are expressed in the distal part of the transition zone. Is reduced basipetal auxin flow involved in inhibition of root elongation? Plant Physiol 122:945–956. doi:10.1104/pp.122.3.945
Lazof DB, Goldsmith JG, Ruffy TW, Linton RW (1994) Rapid uptake of aluminum into cells of intact soybean root tips. A microanalytical study using secondary ion mass spectrometry. Plant Physiol 106:1107–1114
Léon V, Rabier J, Notonier R, Barthlémy R, Moreau X, Madjèbi SB et al (2005) Effects of three nickel salts on geminating seeds of Grevillea exul var rubiginosa, an endemic Serpentine Proteaceae. Ann Bot (Lond) 95:609–618. doi:10.1093/aob/mci066
Liu K, Luan S (2001) Internal aluminum block of plant inward K+ channels. Plant Cell 13:1453–1465
MacDiarmid CW, Gardner RC (1998) Overexpression of the Saccharomyces cerevisiae magnesium transport system confers resistance to aluminum ion. J Biol Chem 273:1727–1732. doi:10.1074/jbc.273.3.1727
Ma JF, Zheng SJ, Li XF, Takeda K, Matsumoto H (1997) A rapid hydroponic screening for aluminium tolerance in barley. Plant Soil 191:133–137. doi:10.1023/A:1004257711952
Miyake K (1916) The toxic action of soluble aluminum salts upon the growth of the rice plant. J Biol Chem 25:23–28
Miyasaka SC, Buta JG, Howell RK, Foy CD (1991) Mechanism of aluminum tolerance in snapbeans. Root exudation of citric acid. Plant Physiol 96:737–743
Parrot WA, Bouton JH (1990) Aluminum tolerance in alfalfa as expressed in tissue culture. Plant Soil 191:133–137
Silva IR, Smyth TJ, Raper CD, Carter TE, Rufty TW (2001) Differential aluminum tolerance in soybean: an evaluation of organic acid. Physiol Plant 112:200–210. doi:10.1034/j.1399-3054.2001.1120208.x
Sivaguru M, Frantisek B, Dieter V, Hubert HF, Walter JH (1999) Impacts of aluminum on the cytoskeleton of the maize root apex. Short-term effects on the distal part of the transition zone. Plant Physiol 119:1073–1082. doi:10.1104/pp.119.3.1073
Taylor GJ, Stephens JLM, Hunter DB, Bertsch PM, Rengel DEZ, Reid RJ (2000) Direct measurement of aluminum uptake and distribution in single cell of Chara corallina. Plant Physiol 123:987–996. doi:10.1104/pp.123.3.987
Tesfaye M, Stephen JT, Deborah LA, Carroll PV, Deborah AS (2001) Overexpression of malate dehydrogenase in transgenic alfalfa enhances organic acid synthesis and confers tolerance to aluminum. Plant Physiol 127:1836–1844. doi:10.1104/pp.127.4.1836
Van Wambeke A (1976) Formation, distribution and consequence of acid soils in agricultural development. In: Wright MJ (ed) Plant adaptation to mineral stress I problem soils. Cornell University Press, Ithaca, pp 15–24
Voigt PW, Staley TE (2004) Selection for aluminum and acid-soil resistance in white clover. Crop Sci 44:38–48
Voigt PW, Morris DR, Godwin HW (1997) A soil-on-agar method to evaluate acid-soil resistance in white clover. Crop Sci 37:1493–1496
Yamamoto Y, Kobayashi Y, Devi SR, Rikiishi S, Matsumoto H (2002) Aluminum toxicity is associated with mitochondrial dysfunction and the production of reactive oxygen species in plant cells. Plant Physiol 128:63–72. doi:10.1104/pp.128.1.63
Ying XF, Liu PX, Gen D, Lu QD, Zhu SL (2005) Screening of soybean genotypes with tolerance to aluminum toxicity and study of the screening indicex. Chin J Oil Crop Sci 27:46–51
Zheng SJ, Ma JF, Matsumoto H (1998) High aluminum resistance in buckwheat. I. Al-induced specific secretion of Oxalic acid from root tips. Plant Physiol 117:745–751. doi:10.1104/pp.117.3.745
Acknowledgements
This study was supported by the project of National Key Basice Research and Development (2002 CB410804) and Planned Science and Technology Project of Zhejiang Province (2007C13063).
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Pan, XB., Zhu, C. & Cheng, C. Assessment of techniques for screening alfalfa cultivars for aluminum tolerance. Euphytica 164, 541–549 (2008). https://doi.org/10.1007/s10681-008-9751-0
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DOI: https://doi.org/10.1007/s10681-008-9751-0