Abstract
This research was conducted to study the genetic diversity in safflower (Carthamus tinctorius L.) using agro-morphological traits and RAPD markers. Sixteen selected lines derived from landraces growing in various agro-climatic regions of Iran along with four exotic genotypes were evaluated in a randomized complete block design with three replications under field conditions. Days to emergence, days to initial flowering, days to flowering, days to maturity, plant height, branches per plant, capitula per plant, seeds per capitulum, 1,000-seed weight, seed yield per plant, seed yield, and reaction to powdery mildew (Leveillula taurica Arnaud) were evaluated in this study. Genetic diversity of the genotypes was assessed by RAPD markers. The results indicated significant differences among genotypes for the agro-morphological traits and clustering based on these traits classified the genotypes into five groups. Analysis of the RAPD markers revealed 15 polymorphic primers out of 50 used primers. Based on RAPD data, the highest genetic similarity was observed between the cultivars of “AC Sunset,” “AC Sterling” from Canada and the lowest relatedness observed between a local breeding line “E2428” and genotype “GE62923” from Germany. Cluster analysis based on RAPD markers and 54% coefficient of similarity divided the genotypes into five distinct groups. Comparing the clusters based on agro-morphological traits with those from molecular markers showed slight similarities. The finding of high genetic variation for agro-morphological traits and polymorphism at DNA level reveal that agronomic traits can be improved by selection programs.
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References
Able GH, Driscoll MF (1976) Sequential trait development and breeding for high yield in safflower. Crop Sci 16:213–216
Anderson JA, Churchill GA, Autroque JE, Tanksley SD, Swells ME (1993) Optimising selection for plant linkage map. Genome 36:181–186
Ash GJ, Raman R, Crump NS (2003) An investigation of genetic variation in Carthamus lanatus in New South Wales, Australia, using intersimple sequence repeats (ISSR) analysis. Weed Res 43:208–213
Ashri A (1975) Evaluation of the germplasm collection of safflower Carthamus tinctorius L. V: Distribution and regional divergence for morphological characters. Euphytica 24:651–659
Ashri A, Zimmer DE, Urie AL, Cahaner A, Marani A (1974) Evaluation of the world collection of safflower (Carthamus tinctorius L). IV. Yield and yield components and their relationship. Crop Sci 14:799–802
Braun U (1987) A monograph of the Erysiphales (Powdery mildews). Beihefte zur Nova Hedwigia 89. J. Cramer, Berlin
Caetano-Anolles G, Bassam BJ, Gresshoff PM (1991) DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Biotech 9:553–556
Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction sites. Genetics 131:479–491
Falconer DS, Mackay TFC (1996) Introduction to quantitative genetics. Longman, Harlow, UK
Fernandez-Martinez J, Rio M, Haro A (1993) Survey of safflower (Carthamus tinctorius L) germplasm for variants in fatty acid composition and other seed characters. Euphytica 19:115–122
Han Y, Li D (1992) Evaluation of safflower (Carthamus tinctorius L.) germplasm-analysis in fatty acid composition of seeds of domestic and exotic safflower varieties. Bot Res 6:28–35
Harlan J (1992) Crops and man, 2nd edn. ASA, CSSA, Inc., Madison, WI, USA
Holiday P (1980) Fungus diseases of tropical crops. Cambridge Univ. Press, Cambridge, UK
Jan CC (1988) Registration of a powdery mildew resistant sunflower germ plasm pool, PM1. Crop Sci 28:1040
Jaradat AA, Shahid M (2006) Patterns of phenotypic variation in a germplasm collection of Carthamus tinctorius L. from the Middle East. Euphytica 53:225–244
Kavani RH, Shukla PT, Madariya RB (2000) Analysis of variability for seed yield and related characters in safflower (Carthamus tinctorius L.). Madras Agric J 87:449–452
Kearsey MJ, Pooni HS (1996) The genetical analysis of quantitative traits. Chapman and Hall, London
Knowles PF (1989) Centers of plant diversity and conservation of crop germplasm: safflower. Econ Bot 23:324–329
Knowles PF, Ashri A (1995) Safflower Carthamus tinctorius (Compositae). In: Smartt J, Simmonds NW (eds) Evolution of crop plants, 2nd edn. Longman Scientific and Technical, London, pp 47–50
Lahane PS, Mukewar AM, Zope JS, Kalpande HV, Kalpande VV (1999) Genetic variability for different traits in safflower (Carthamus tinctorius L.). J Soil Crops 9:130–132
Maali Amiri RM, Yazdi-Samadi B, Ghannadha MR, Abd-Mishani C (2001) Detection of DNA polymorphism in landrace populations of safflower in Iran using RAPD-PCR technique. Iran J Agri Sci 32:737–745
Mantel N (1967) The detection of disease clustering and a generalized regression approach. Cancer Res 27:209–220
Murray MG, Thampson WF (1998) Rapid isolation of high molecular weight plant DNA. Nucleic Acid Res 8:4321–4325
Nei M, Li WH (1979) Mathematical model for studying in genetic variation. Proc Natl Acad Sci USA 76:5269–5273
Rohlf FJ (1972) An empirical comparison of three ordination techniques in numerical taxonomy. Syst Zool 21:271–280
Rohlf FJ (1992) NTSYS-PC: numerical taxonomy and multivariate analysis system version 2.02 k. State University of New York, Stony Brook, NY
SAS Institute (1997) SAS/STAT Software: changes and enhancements through Release 6.12. SAS Institute Inc., Cary, NC
Sehgal D, Raina SN (2005) Genotyping safflower (Carthamus tinctorius) cultivars by DNA fingerprints. Euphytica 146:67–76
Vilatersana R, Garnatje T, Susanna A, Garcia-Jacas N (2005) Taxanomic problems in Carthamus (Asteraceae): RAPD markers and sectional classification. J Bot Linn Soc 147:375–385
Weiss EA (2000) Oil Seed Crops. Blackwell Science Ltd, Oxford, UK
Williams JGK, Kubelik AR, Livak KJ, Rafaleski JA, Tingey SV (1990) DNA Polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535
Yu-Xia Y, Wei W, You-Liang Z, Li C, Ren-Jian L, Chun-Yan H (2007) Genetic diversity and relationships among safflower (Carthamus tinctorius L.) analyzed by inter-simple sequence repeats (ISSRs). Genet Res Crop Evol 54:1043–1051
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Amini, F., Saeidi, G. & Arzani, A. Study of genetic diversity in safflower genotypes using agro-morphological traits and RAPD markers. Euphytica 163, 21–30 (2008). https://doi.org/10.1007/s10681-007-9556-6
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DOI: https://doi.org/10.1007/s10681-007-9556-6