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Establishment of Chinese soybean Glycine max core collections with agronomic traits and SSR markers

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Abstract

It is very important to efficiently study and use genetic diversity resources in crop breeding and sustainable agriculture. In this study, different sampling methods and sample sizes were compared in order to optimize the strategies for building a rationally sized core collection of Chinese soybean (Glycine max). The diversity in the core collection captured more than 70% of that in the pre-core collection, no matter what sampling methods were used, at a sampling proportion of 1%. Core collections established with both simple sequence repeat (SSR) marker data and agronomic traits were more representative than those chosen on an independent basis. An optimal sampling method for a soybean core collection was determined, in which strategy ‘S’ (allocating accessions to clusters according to the proportion of square root of the original sample size within each ecotype) was used based on SSR and agronomic data. Curve estimation was used to estimate the allelic richness of the entire Chinese soybean germplasm and a minimum sample size for a core collection, on which a sampling proportion of about 2% was determined to be optimal for a core collection. Further analysis on the core collection with fourteen agronomic traits and allelic constitution at 60 SSR loci suggested that it highly represented the entire collections both on genetic structure and diversity distribution. This core collection would provide an effective platform in proper exploitation of soybean germplasm resources for the study of complex traits and discovering important novel traits for crop genetic development.

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Authors and Affiliations

  1. Institute of Crop Science, National Key Facility of Crop Gene Resource and Genetic Improvement, Key Lab of Crop Germplasm & Biotechnology (MOA), Chinese Academy of Agricultural Sciences, 100081, Beijing, China

    Lixia Wang, Rongxia Guan, Yinghui Li, Zhangxiong Liu, Ruzhen Chang & Lijuan Qiu

  2. Xinjiang Agricultural University, 830046, Urumqi, China

    Yuan Guan, Haiyan Zhang, Yueqiang Zhang, Zhe Yan, Xuecheng Ning & Li Zhu

  3. Department of agronomy, Jilin Agricultural University, 130118, Changchun, China

    Yansong Ma

  4. Department of Agronomy, Liaoning Agricultural University, 110161, Shenyang, China

    Zhimin Dong

  5. Northeast Agricultural University, 150030, Haerbin, China

    Xian Liu, Yanhua Cui, Rihua Piao & Yan Liu

  6. Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, 310029, Hangzhou, China

    Haiming Xu

  7. Northwest Science and Technology, University of Agriculture & Forestry, Yangling, 712100, Shannxi, China

    Linhai Li

  8. College of Agriculture Northwest Sci-Tech, University Agriculture and Forestry, Shanxi Yanglin, 712100, Shannxi, China

    Fanyun Lin & Weijiang Luan

  9. Department of Crop, Soil, and Environmental Sciences, University of Arkansas, Fayetteville, AR, 72701, U.S.A.

    Pengying Chen

Authors
  1. Lixia Wang
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  2. Yuan Guan
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  3. Rongxia Guan
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  4. Yinghui Li
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  5. Yansong Ma
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  6. Zhimin Dong
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  7. Xian Liu
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  8. Haiyan Zhang
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  9. Yueqiang Zhang
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  10. Zhangxiong Liu
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  11. Ruzhen Chang
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  12. Haiming Xu
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  13. Linhai Li
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  14. Fanyun Lin
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  15. Weijiang Luan
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  16. Zhe Yan
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  17. Xuecheng Ning
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  18. Li Zhu
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  19. Yanhua Cui
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  20. Rihua Piao
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  21. Yan Liu
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  22. Pengying Chen
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  23. Lijuan Qiu
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Corresponding author

Correspondence to Lijuan Qiu.

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Wang, L., Guan, Y., Guan, R. et al. Establishment of Chinese soybean Glycine max core collections with agronomic traits and SSR markers. Euphytica 151, 215–223 (2006). https://doi.org/10.1007/s10681-006-9142-3

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  • DOI: https://doi.org/10.1007/s10681-006-9142-3

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