Abstract
To evaluate the effect of low-molecular-weight glutenin subunit (LMW-GS) genes on dough strength, locus-specific primers of LMW-GS genes and gliadin bands tightly linked to LMW-GS genes were analyzed in common wheat. Segregation analysis of the F2 progeny from a cross between ‘Haruhikari’, a good bread-making quality cultivar, and ‘Asakaze-komugi’, a poor bread-making quality cultivar, showed that dough strength significantly correlated with one amplified LMW-GS gene located at the Glu-B3 locus from ‘Haruhikari’. There was no specific reference to the gliadin bands identified as promising markers in the cross under study. The LMW-GS gene of ‘Haruhikari’ had a seven amino-acid deletion in the repetitive domain relative to ‘Asakaze-komugi’, as well as six amino-acid substitutions, three of which would be expected to cause changes in hydrophilicity. The presence of the LMW-GS gene and other LMW-GS genes tightly linked to it may affect the dough strength of wheat.
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Blanco, A., C. De Giovanni, B. Laddomada, A. Sciancalepore, R. Simeone, K.M. Devos & M.D. Gale, 1996 Quantitative trait loci influencing grain protein content in tetraploid wheats. Plant Breed 115: 310–316.
Dholakia, B.B., J.S. Ammiraju, D.K. Santra, H. Singh, M.V. Katti, M.D. Lagu, S.A. Tamhankar, V.S. Rao, V.S. Gupta, H.S. Dhaliwal & P.K. Ranjekar, 2001 Molecular marker analysis of protein content using PCR-based markers in wheat. Biochem Genet 39: 325–338.
D’Ovidio, R., O.A. Tanzarella & E. Porceddu, 1992 Nucleotide sequence of a low-molecular-weight glutenin from Triticum durum. Plant Molec Biol 18: 781–784.
D’Ovidio, R., M. Simeone, S. Masci & E. Porceddu, 1997 Molecular characterization of a LMW-GS gene located on chromosome 1B and the development of primers specific for the Glu-B3 complex locus in durum wheat. Theor Appl Genet 95: 1119–1126.
Duncan, D.B. 1955 Multiple range and multiple F tests. Biometrics 11: 1–42.
Graβ berger, A., P. Schieberle & P. Koehler, 2003 Fractionation and reconstitution of wheat flour-effect on dough rheology and baking. Eur Food Res Technol 216: 204–211.
Harjit-Singh, M. Prasad, R.K. Varshney, J.K. Roy, H.S. Balyan, H.S. Dhaliwal & P.K. Gupta, 2001 STMS markers for grain protein content and their validation using near-isogenic lines in bread wheat. Plant Breed 120: 273–278.
Ikeda, T.M., T. Nagamine, H. Fukuoka & H. Yano, 2002 Identification of new low-molecular-weight glutenin subunit genes in wheat. Theor Appl Genet 104: 680–687.
Khan, I.A., J.D. Procunier, D.G. Humphreys, G. Tranquilli, A.R. Schlatter, S. Marcucci-Poltri, R. Frohberg & J. Dubcovsky, 2000 Development of PCR-based markers for a high grain protein content gene from Triticum turgidum ssp. dicoccoides transferred to bread wheat. Crop Sci 40: 518–524.
Mesfin, A., R.C. Frohberg & J.A. Anderson, 1999 RFLP markers associated with high grain protein from Triticum turgidum L. var. dicoccoides introgressed into hard red spring wheat. Crop Sci 39: 508–513.
Metakovsky, E.V. & A. Yu Novoselskaya, 1991 Gliadin allele identification in common wheat. I. Methodological aspects of the analysis of gliadin patterns by one-dimensional polyacrylamide gel electrophoresis. J Genet Breed 45: 317–324.
Moonen, J.H.E., A. Scheepstra & A. Graveland, 1982 Use of the SDS-sedimentation test and SDS-polyacrylamide gel electrophoresis for screening breeder’s samples of wheat for bread-making quality. Euphytica 31: 677–690.
Murray, H.G. & W.F. Thompson, 1980 Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8: 4321–4325.
Payne, P.I., K.G. Corfield & J.A. Blackman, 1979 Identification of a high-molecular-weight subunit of glutenin whose presence correlates with bread-making quality in wheats of related pedigree. Theor Appl Genet 55: 153–159.
Payne, P.I., K.G. Corfield, L.M. Holt & J.A. Blackman, 1981 Correlations between the inheritance of certain high-molecular-weight subunits of glutenin and bread-making quality in progenies of six crosses of bread wheat. J Sci Food Agric 32: 51–60.
Payne, P.I., M.A. Nightingale, A.F. Krattiger & L.M. Holt 1987a The Relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties. J Sci Food Agric 40: 51–65.
Payne, P.I., J.A. Seekings, A.J. Worland, M.G. Jarvis & L.M. Holt 1987b Allelic variation of glutenin subunits and gliadins and its effect on bread making quality in wheat: Analysis of F 5 progeny from Chinese Spring × Chinese Spring (Hope 1A). J Cereal Sci 6: 103–118.
Prasad, M., R.K. Varshney, A. Kumar, H.S. Balyan, P.C. Sharma, K.J. Edwards, H. Singh, H.S. Dhaliwal, J.K. Roy & P.K. Gupta, 1999 A microsatellite marker associated with a QTL for grain protein content on chromosome arm 2DL of bread wheat. Theor Appl Genet 99: 341–345.
Prasad, M., N. Kumar, P.L. Kulwal, M.S. Roder, H.S. Balyan, H.S. Dhaliwal & P.K. Gupta, 2003 QTL analysis for grain protein content using SSR markers and validation studies using NILs in bread wheat. Theor Appl Genet 106: 659–667.
Sears, E.R. 1954 The aneuploids of common wheat. Mo Agr Exp Stn Res Bull 572: 1–59.
Takata, K., H. Yamauchi, N. Iriki & T. Kuwabara, 1999 Prediction of bread-making quality by prolonged swelling SDS-sedimentation test. Breed Sci 49: 221–223.
Tanaka, H., N. Nakata, M. Osawa, M. Tomita, H. Tsujimoto & Y. Yasumuro 2003a Positive effect of the high-molecular-weight glutenin allele, Glu-D1d, on the bread-making quality of common wheat. Plant Breed 122: 279–280.
Tanaka, H., M. Tomita, H. Tsujimoto & Y. Yasumuro 2003b Limited but specific variations of seed storage proteins in Japanese common wheat (Triticum aestivum L.). Euphytica 132: 167–174.
Van Campenhout, S., J. Vander Stappen, L. Sagi & G. Volckaert, 1995 Locus-specific primers for LMW glutenin genes on each of the group 1 chromosomes of hexaploid wheat. Theor Appl Genet 91: 313–319.
Zaman, Z. & R.L. Verwilghen, 1979 Quantitation of proteins solubilized in sodium dodecyl sulfate-mercaptoethanol-Tris electrophoresis buffer. Anal Biochem 100: 64–69.
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Tanaka, H., Shimizu, R. & Tsujimoto, H. Genetical analysis of contribution of low-molecular-weight glutenin subunits to dough strength in common wheat (Triticum aestivum L.). Euphytica 141, 157–162 (2005). https://doi.org/10.1007/s10681-005-6714-6
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DOI: https://doi.org/10.1007/s10681-005-6714-6