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Quantitative trait loci (QTL) of stem strength and related traits in a doubled-haploid population of wheat (Triticum aestivum L.)

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Abstract

Scoring for lodging resistance is difficult under natural field conditions. The stem strength of wheat has been used as an index of lodging resistance. However, this is a complex trait comprised of two characters, i.e. stem mechanical elasticity and rigidity. Therefore it is closely associated with stem morphological and anatomical features. A study of the genetics of stem strength and related traits of basal stem internodes is very important for genetic improvement of lodging resistance in wheat. In this study, a doubled-haploid (DH) population derived from anther culture of the cross CA9613/H1488 was used. Stem strength and related basal internode traits were measured at the milk stage. A molecular map of the DH population was constructed using 189 SSR markers, and quantitative trait loci (QTL) for each trait were analyzed based on this molecular linkage map. A total of six QTL for stem strength, culm wall thickness, pith diameter and stem diameter were identified: 1) Two QTL (QSs-3A and QSs-3B) for stem strength were detected on chromosomes 3A and 3B, exhibiting 10.6 and 16.6% phenotypic variance, respectively. 2) Two QTL (QPd-1A and QPd-2D) associated with pith diameter were detected on chromosomes 1A and 2D, respectively, jointly explaining about 30% of phenotypic variance. 3) As far as stem diameter and culm wall thickness were concerned, one QTL was detected on chromosomes 3B and 2D, respectively; QSd-3B explained 8.7% of the phenotypic variance of stem diameter, whereas QCwt-2D explained 9.6% of the phenotypic variance of culm wall thickness. In addition, among the QTLs detected, two with pleiotropic effects were observed. Correlated traits are usually associated with the pleiotropic effects of the same QTL(s) or linkage of different QTLs. But this was not true in some cases. The results of QTL mapping showed that stem strength can be improved by breeding for wider stems with a higher stem diameter/pith diameter ratio. This can be facilitated by using the markers linked to QSd-3B and QCwt-2D. Combining stem strength, stem diameter and culm wall thickness may be used as a selection index for lodging resistance with marker-assisted selection (MAS) to improve lodging resistance in this population.

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Abbreviations

CIM:

composite interval mapping

CWT:

culm wall thickness of basal internode

DH:

doubled-haploid population

LOD:

log10 likelihood value

MAS:

marker-assisted selection

PCR:

polymerase chain reaction

PD:

pith diameter of basal internode

RILs:

recombinant inbred lines

QTL:

quantitative trait loci

SD:

stem diameter of basal internode

SS:

stem strength

SSR:

simple sequence repeat

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Authors and Affiliations

  1. Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture/National Centre for Wheat Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing, 100081, P.R. China

    Lin Hai, Huijun Guo, Shihe Xiao, Xiuying Zhang, Changsheng Yan, Zhiyong Xin & Jizeng Jia

  2. Department of Plant Breeding, Justus – Liebig – University Giessen, Heinrich - Buff Ring 26–32, 35392, Giessen, Germany

    Lin Hai

  3. Department of Crop and Soil Sciences, Michigan State University, East Lansing, Michigan, 48824, U.S.A.

    Guoliang Jiang

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  1. Lin Hai
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  2. Huijun Guo
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  3. Shihe Xiao
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  4. Guoliang Jiang
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  5. Xiuying Zhang
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  6. Changsheng Yan
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  7. Zhiyong Xin
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Correspondence to Shihe Xiao.

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Hai, L., Guo, H., Xiao, S. et al. Quantitative trait loci (QTL) of stem strength and related traits in a doubled-haploid population of wheat (Triticum aestivum L.). Euphytica 141, 1–9 (2005). https://doi.org/10.1007/s10681-005-4713-2

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