Skip to main content
SpringerLink
Log in

Estimation of deoxynivalenol (DON) content by symptom rating and exoantigen content for resistance selection in wheat and triticale

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Fusarium head blight (FHB) in wheat and triticale leads to contamination of the grain with the mycotoxin deoxynivalenol (DON) that is harmful to animal and man. A fast, low-cost, and reliable method for quantification of the DON content in the grain is essential for selection. We analysed 113 wheat and 55 triticale genotypes for their symptom development on spikes, Fusarium exoantigen (ExAg) and DON content in the grain after artificial inoculation with a highly aggressive isolate of F. culmorum in three (wheat) and six (triticale) location-by-year combinations. Additionally, in triticale the amount of Fusarium damaged kernels (FDK) was assessed. ExAg content was analysed by a newly developed Fusarium-specific plate-trapped antigen enzyme-linked immunosorbent assay (PTA-ELISA) and DON content by an immunoassay. A moderate disease severity resulted in an ExAg content of 0.87 optical density (OD) units in wheat and 1.02 OD in triticale. DON content ranged from 12.0 to 105.2 mg kg−1 in wheat and from 24.2 to 74.0 mg kg−1 in triticale. Genotypic and genotype-by-environment interaction variances were significant (P < 0.01). Coefficient of phenotypic correlation between DON content analysed by the immunoassay and ExAg content was r = 0.86 for wheat and r = 0.60 for triticale. The highest correlation between DON content and symptom rating was found by FHB rating in wheat (r = 0.77) and by FDK rating in triticale (r = 0.71). In conclusion, selection for reduced FHB symptoms should lead to a correlated selection response in low fungal biomass and low DON content in the grain.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • D., Abramson, Z. Gan, R.M. Clear, J. Gilbert & R.R. Marquardt, 1998. Relationships among deoxynivalenol, ergosterol and Fusarium exoantigens in Canadian hard and soft wheat. Int J Food Microbiol 45: 217–224.

    PubMed  CAS  Google Scholar 

  • Bai, G.H., G. Shaner & H. Ohm, 2000. Inheritance of resistance to Fusarium graminearum in wheat. Theor Appl Genet 100: 1–8.

    Google Scholar 

  • Bai, G.H., R. Plattner, A. Desjardins & F. Kolb, 2001. Resistance to Fusarium head blight and deoxynivalenol accumulation in wheat. Plant Breeding 120: 1–6.

    CAS  Google Scholar 

  • Banks, J.N. & S.H. Cox, 1992. The solid phase attachment of fungal hyphae in an ELISA to screen for antifungal antibodies. Mycopathologia 120: 79–85.

    PubMed  CAS  Google Scholar 

  • J., Chelkowski, 1989. Formation of mycotoxins produced by Fusaria in heads of wheat, triticale, and rye. In: J. Chelkowski (Ed.), Fusarium – Mycotoxins, Taxonomy, and Pathogenicity, pp. 63–84. Elsevier, Amsterdam.

    Google Scholar 

  • Cochran, W.G. & G.M. Cox, 1957. Experimental Designs, 2nd edn. John Wiley & Sons, New York.

    Google Scholar 

  • C., Danks, S.A. Ostoja-Starzewska, R.H. Rizvi & J.N. Banks, 2001. Development of rapid assays for detection of fungi and mycotoxins. Mycotoxins 51: 41–49.

    Article  CAS  Google Scholar 

  • D’Mello, J.P.F., C.M. Placinta & A.M.C. Macdonald, 1999. Fusarium mycotoxins: A review of global implications for animal health, welfare and productivity. Anim Feed Sci Technol 80: 183–205.

    Google Scholar 

  • Fehr, W.R., 1987. Principles of Cultivar Development, Theory and Technique, Vol. 1. Macmillan, New York, USA.

    Google Scholar 

  • Foroughi-Wehr, B., V. Lind, S. Züchner & F. Rabenstein, 1995. Different assessment techniques of leaf blotch (Rhynchosporium secalis (Oud.) J. Davis) in winter barley after artificial inoculation. J Phytopathol 143: 553–559.

    Google Scholar 

  • Z., Gan, R.R. Marquardt, D. Abramson & R. Clear, 1997. The characterization of antibodies raised against Fusarium spp. by enzyme-linked immunosorbent assay and immunoblotting. Int J Food Microbiol 38: 191–200.

    PubMed  CAS  Google Scholar 

  • G., Gang, T. Miedaner, U. Schuhmacher, M. Schollenberger & H.H. Geiger, 1998. Deoxynivalenol and nivalenol production by Fusarium culmorum isolates differing in aggressiveness toward winter rye. Phytopathology 88: 879–884.

    PubMed  CAS  Google Scholar 

  • S., Homdork, H. Fehrmann & R. Beck, 2000. Influence of different storage conditions on the mycotoxin production and quality of Fusarium-infected wheat grain. J Phytopathol 148: 7–15.

    CAS  Google Scholar 

  • Hart, L.P., H. Casper, O. Schabenberger & P. Ng, 1998. Comparison of gas chromatography and enzyme-linked immunosorbent assay for deoxynivalenol in milled fractions of naturally contaminated wheat. J Food Protec 61: 1695–1697.

    CAS  Google Scholar 

  • Hollins, T.W., P. Ruckenbauer & H. De Jong, 2003. Progress towards wheat varieties with resistance to Fusarium head blight. Food Control 14: 239–244.

    Google Scholar 

  • Kaufman, L. & P.G. Standard, 1987. Specific and rapid identification of medically important fungi by exoantigen detection. Ann Rev Microbiol 41: 209–225.

    CAS  Google Scholar 

  • Leonard, K.J. & W.R. Bushnell, 2003. Fusarium Head Blight of Wheat and Barley. APS Press, St. Paul, Minnesota, USA.

    Google Scholar 

  • Li, S.Z., R.R. Marquardt & D. Abramson, 2000. Immunochemical detection of molds: A review. J Food Protec 63: 281–291.

    CAS  Google Scholar 

  • K., Lienemann, 2002. Auftreten von Fusarium-Arten an Winterweizen im Rheinland und Möglichkeiten der Befallskontrolle unter besonderer Berücksichtigung der Weizensorte. Ph.D. Thesis, University of Bonn, Germany.

  • MesterhÁzy, Á., 1995. Types and components of resistance to Fusarium head blight of wheat. Plant Breeding 114: 377–386.

    Google Scholar 

  • MesterhÁzy, Á., 2002. Role of deoxynivalenol in aggressiveness of Fusarium graminearum and F. culmorum and in resistance to Fusarium head blight. Eur J Plant Pathol 108: 675–684.

    Google Scholar 

  • MesterhÁzy, Á., T. Bartók, C.G. Mirocha & R. Komoróczy, 1999. Nature of wheat resistance to Fusarium head blight and the role of deoxynivalenol for breeding. Plant Breeding 118: 97– 110.

    Google Scholar 

  • T., Miedaner, 1997. Breeding wheat and rye for resistance to Fusarium diseases. Plant Breeding 116: 201–220.

    Google Scholar 

  • Miedaner, T. & J. Perkowski, 1996. Correlations among Fusarium culmorum head blight resistance, fungal colonization and mycotoxin content in winter rye. Plant Breeding 115: 347–351.

    CAS  Google Scholar 

  • T., Miedaner, C. Reinbrecht, U. Lauber, M. Schollenberger & H.H. Geiger, 2001a. Effects of genotype and genotype × environment interaction on deoxynivalenol accumulation and resistance to Fusarium head blight in rye, triticale, and wheat. Plant Breeding 120: 97–105.

    CAS  Google Scholar 

  • T., Miedaner, A.G. Schilling & H.H. Geiger, 2001b. Molecular genetic diversity and variation for aggressiveness in populations of Fusarium graminearum and Fusarium culmorum sampled from wheat fields in different countries. J Phytopathol 149: 641– 648.

    CAS  Google Scholar 

  • T., Miedaner, B. Schneider & H.H. Geiger, 2003. Deoxynivalenol (DON) content and Fusarium head blight resistance in segregating populations of winter rye and winter wheat. Crop Sci 43: 519– 526.

    Article  CAS  Google Scholar 

  • Müller, H.-M., J. Reimann, U. Schumacher & K. Schwadorf, 1997. Fusarium toxins in wheat harvested during six years in an area of southwest Germany. Natural Toxins 5: 24–30.

    PubMed  Google Scholar 

  • Oettler, G. & G. Wahle, 2001. Genotypic and environmental variation of resistance to head blight in triticale inoculated with Fusarium culmorum. Plant Breeding 120: 297–300.

    Google Scholar 

  • Parry, D.W., P. Jenkinson & L. McLeod, 1995. Fusarium ear blight (scab) in small grain cereals – A review. Plant Pathol 44: 207– 238.

    Google Scholar 

  • Placinta, C.M., J.P.F. D’Mello & A.M.C. Macdonald, 1999. A review of worldwide contamination of cereal grains and animal feed with Fusarium mycotoxins. Anim Feed Sci Technol 78: 21– 37.

    CAS  Google Scholar 

  • F., Rabenstein, 2002. Development of serological methods for detection of Fusarium species in barley and wheat grains. Annual Report of BAZ for 2001, Federal Centre for Breeding Research on Cultivated Plants, Aschersleben, Germany.

  • Rudd, J.C., R.D. Horsley, A.L. McKendry & E.M. Elias, 2001. Host plant resistance genes for Fusarium head blight: Sources, mechanisms, and utility in conventional breeding systems. Crop Sci 41: 620–627.

    Article  Google Scholar 

  • H., Schnerr, R.F. Vogel & L. Niessen, 2002. Correlation between DNA of trichothecene-producing Fusarium species and deoxynivalenol concentrations in wheat samples. Lett Appl Microbiol 35: 121–125.

    PubMed  CAS  Google Scholar 

  • M., Schollenberger, U. Lauber, H.T. Jara, S. Suchy, W. Drochner & H.-M. Müller, 1998. Determination of eight trichothecenes by gas chromatography–mass spectrometry after sample clean-up by a two-stage solid-phase extraction. J Chromatogr A 815: 123– 132.

    Article  CAS  PubMed  Google Scholar 

  • M., Schollenberger, U. Lauber, H.T. Jara, S. Suchy, W. Drochner & H.-M. Müller, 2002. Fusarium toxins in wheat flour collected in an area in southwest Germany. Int J Food Microbiol 72: 85– 89.

    PubMed  CAS  Google Scholar 

  • Sinha, R.C. & M.E. Savard, 1996. Comparison of immunoassay and gas chromatography methods for the detection of the mycotoxin deoxynivalenol in grain samples. Can J Plant Pathol 18: 233– 236.

    Article  CAS  Google Scholar 

  • Snedecor, G.W. & W.G. Cochran, 1989. Statistical Methods, 8th edn. Iowa State University, Ames.

    Google Scholar 

  • Snijders, C.H.A., 1990. The inheritance of resistance to head blight caused by Fusarium culmorum in winter wheat. Euphytica 50: 11–18.

    Article  Google Scholar 

  • Snijders, C.H.A. & J. Perkowski, 1990. Effects of head blight caused by Fusarium culmorum on toxin content and weight of wheat kernels. Phytopathology 80: 566–570.

    CAS  Google Scholar 

  • Utz, H.F., 2000. PLABSTAT: A Computer Programme for Statistical Analysis of Plant Breeding Experiments, Version 2N. Institute of Plant Breeding, Seed Science, and Population Genetics, University of Hohenheim, Stuttgart, Germany.

Download references

Author information

Authors and Affiliations

  1. State Plant Breeding Institute, University of Hohenheim, D-70593, Stuttgart, Germany

    T. Miedaner, N. Heinrich, B. Schneider & G. Oettler

  2. Federal Center for Breeding Research on Cultivated Plants, Institute of Resistance Research and Pathogen Diagnostics, P.O. Box 1505, D-06435, Aschersleben, Germany

    S. Rohde & F. Rabenstein

Authors
  1. T. Miedaner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. Heinrich
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. Schneider
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Oettler
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. Rohde
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. F. Rabenstein
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to T. Miedaner.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Miedaner, T., Heinrich, N., Schneider, B. et al. Estimation of deoxynivalenol (DON) content by symptom rating and exoantigen content for resistance selection in wheat and triticale. Euphytica 139, 123–132 (2004). https://doi.org/10.1007/s10681-004-2489-4

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-004-2489-4

Key words

Search

Navigation