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Similarities and differences in combined toxicity of sulfonamides and other antibiotics towards bacteria for environmental risk assessment

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Abstract

Antibiotics as a type of environmental contaminants are typically exposed to chemical mixtures over long periods of time, so chronic combined toxicity is the best way to perform an environmental risk assessment. In this paper, the individual and combined toxicity of sulfonamides (SAs), sulfonamide potentiators (SAPs), and doxycycline hyclate (DH) were tested on gram-positive (Bacillus subtilis, B. subtilis) and gram-negative (Escherichia coli, E. coli) bacteria. The individual toxicity of antibiotics on the two bacteria could be ranked in the same order: SAs < SAPs < DH. But E. coli was more sensitive than B. subtilis to the antibiotics, which was likely due to both the different abilities of antibiotics to pass through the cell membrane and the varied capacities to bind target proteins between the two bacteria. In addition, the binary mixtures of SAs–SAPs, SAs–DH, and SAs–SAs exhibited synergistic, antagonistic, and additive effects on both of the bacteria but in different magnitudes as represented by the toxicity units (TU). And we found the different TU values were result from the different effective concentrations of antibiotic mixtures based on the approach of molecular docking and quantitative structure–activity relationships (QSARs). Moreover, from the results of risk assessment, it should be noted that the mixture of SAs and other antibiotics may pose a potential environmental risk assessment due to their combined action with the current environmentally realistic concentrations.

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Acknowledgments

This work was supported by the State Key Laboratory of Pollution Control and Resource Reuse, China, under Grant PCRRY11003, the National Natural Science Foundation of China, under Grant 21377096 and 21577105, the “Climbing” Program of Tongji University, under Grant 0400219287, the 111 Project, and the Science & Technology Commission of Shanghai Municipality, under Grant 14DZ2261100.

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Authors and Affiliations

  1. State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, 17, Shanghai, 200092, China

    Shuxia Fang, Dali Wang, Xiaoxian Zhang, Xi Long, Mengnan Qin & Zhifen Lin

  2. Shanghai Key Lab of Chemical Assessment and Sustainability, Shanghai, China

    Zhifen Lin & Ying Liu

  3. Collaborative Innovation Center for Regional Environmental Quality, Beijing, China

    Zhifen Lin

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  1. Shuxia Fang
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  2. Dali Wang
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  5. Mengnan Qin
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  6. Zhifen Lin
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  7. Ying Liu
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Correspondence to Zhifen Lin.

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Fang, S., Wang, D., Zhang, X. et al. Similarities and differences in combined toxicity of sulfonamides and other antibiotics towards bacteria for environmental risk assessment. Environ Monit Assess 188, 429 (2016). https://doi.org/10.1007/s10661-016-5422-0

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