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What types of powdery mildew can infect wheat-barley introgression lines?

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Abstract

This work is a detailed study of the infection of fungal biotrophic pathogens causing powdery mildew diseases on introgression lines originating from the intergeneric hybridisation between wheat and barley (Triticum aestivum L. × Hordeum vulgare L.). Powdery mildew fungi are among the most widespread biotrophic pathogens of plants also and infect dicot and monocot species. Most powdery mildew species are strictly host specific. They colonize only a narrow range of species or one particular host species. The intergeneric hybridisation between wheat and barley could result in expansions of host ranges of the barley powdery mildew. Our experiments covered natural infections in the field and artificial infections under greenhouse conditions. Formae speciales of powdery mildew were identified on the basis of the sequencing results of ribosomal internal transcribed spacer sequences (rDNA-ITS). We identified Blumeria graminis f.sp. tritici isolate 14 (HM484334) on the wheat parent and all wheat-barley introgression lines and B. g. f. sp. hordei isolate MUMH1723 (AB 273556) on the barley parent, respectively. The wheat-barley introgression lines were inoculated with barley powdery mildew under greenhouse conditions. According to our results the added barley chromosomes (or segments) do not cause host range expansion of barley powdery mildew.

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Acknowledgments

The present publication was realized with the support of the project TÁMOP 4.2.4.A/2-11-1-2012- 0001 and TÁMOP -4.2.2. A-11/1 KONV-2012-0064. The project was realized with the support of the Hungarian Government and the European Union, with the co-funding of the European Social Fund. István Cernák is supported by the János Bolyai Research Fellowship of the Hungarian Academy of Sciences. Barley powdery mildew isolates were kindly provided by Dr. Balázs Barna, Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest.

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Authors and Affiliations

  1. Department of Plant Sciences and Biotechnology, Georgikon Faculty, University of Pannonia, Festetics 7, Keszthely, 8360, Hungary

    Nikolett Réka Aranyi, Ildikó Varga & Borbála Hoffmann

  2. Department of Biosciences, University of Helsinki, Helsinki, Finland

    Ildikó Varga & Péter Poczai

  3. Potato Research Centre, Centre of Agricultural Sciences, University of Pannonia, Keszthely, Hungary

    István Cernák

  4. Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary

    Nikolett Réka Aranyi, Gyula Vida & Márta Molnár-Láng

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  1. Nikolett Réka Aranyi
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  2. Ildikó Varga
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  3. Péter Poczai
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  4. István Cernák
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  5. Gyula Vida
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  6. Márta Molnár-Láng
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  7. Borbála Hoffmann
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Correspondence to Borbála Hoffmann.

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Aranyi, N.R., Varga, I., Poczai, P. et al. What types of powdery mildew can infect wheat-barley introgression lines?. Eur J Plant Pathol 139, 19–25 (2014). https://doi.org/10.1007/s10658-014-0382-0

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  • DOI: https://doi.org/10.1007/s10658-014-0382-0

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