Abstract
Seventeen field surveys were done in four commercial orchards during six consecutive fruit-growing seasons from 2006 until 2011 in order to determine the current frequencies of occurrence of M. laxa, M. fructigena, and M. fructicola and their relative contributions to postharvest brown rot in peaches and nectarines in the Ebro Valley. The relative frequencies of occurrence of Monilinia spp. were determined on three sources of primary inoculum and on three sources of secondary inoculum. The major relative frequencies of Monilinia spp. were significantly recorded (P = 0.05) from mummified fruit on the trees (approx. 42 %) and 7-day-old harvested fruit with brown rot (32 %), followed by that recovered from mummified fruit on the orchard bed (14 %), pruned branches on the orchard bed (8 %) and latent infections of immature fruit (3 %). We found that: (a) the relative frequency of M. fructicola has increased over the years to coexist on the same level as at the time M. laxa, (b) M. fructigena is no longer a cause of brown rot in harvested peaches, (c), a progressive reduction in the time of the first appearance of Monilinia airborne conidia (r = −0.30, P = 0.003), and the time of the first latent infection (r = −0.44, P = 0.0001) was detected along years after correlation analysis, and (d) these displacements are not associated with an increased incidence of brown rot disease. The M. fructicola increase was due to its significantly increased presence in 7-day-old harvested fruit with brown rot (r = 0.73, P = 0.0009), in latent infections of immature fruit (r = 0.68, P = 0.002), on pruned branches on the orchard bed (r = 0.56, P = 0.018), and on mummified fruit sampled on the trees (r = 0.53, P = 0.03). This progressive increase was accompanied by a progressive reduction in the relative frequency of occurrence of M. laxa in 7-day-old harvested fruit with brown rot (r = −0.55, P = 0.021) and M. fructigena on mummified fruit sampled on the trees (r = −0.51, P = 0.03).
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References
Boehm, E. W. A., Ma, Z., & Michailides, T. J. (2001). Species-specific detection of Monilinia fructicola from California stone fruits and flowers. Phytopathology, 91, 428–439.
Byrde, R. J., & Willetts, H. J. (1977). The brown rot fungi of fruit. Their biology and control. Oxford: Pergamon Press.
De Cal, A., & Melgarejo, P. (1999). Effects of long-wave UV light on Monilinia growth and identification of species. Plant Diseases, 83, 62–65.
De Cal, A., Gell, I., Usall, J., Viñas, I., & Melgarejo, P. (2009). First report of brown rot caused by Monilinia fructicola in peach orchards in Ebro Valley. Spain Plant Diseases, 93, 763.
Duchoslavová, J., Sirucková, I., Zapletalová, E., Navratil, M., & Safarova, D. (2007). First report of brown rot caused by Monilinia fructicola on various stone and pome fruits in the Czech Republic. Plant Diseases, 91, 907.
EFSA Panel on Plant Health. (2011). Pest risk assessment of Monilinia fructicola for the EU territory and identification and evaluation of risk management options. EFSA Journal, 9(4), 2119.
Elmer, P. A. G., & Gaunt, R. E. (1993). Effect of frequency of dicarboximide applications on resistant populations of Monilinia fructicola and brown rot in New-Zealand orchards. Crop Protection, 12, 83–88.
EPPO (2007). List of A2 pests regulated as quarantine pests in the EPPO region. OEPP/EPPO from http://www.eppo.org/QUARANTINE/listA2.htm.
Free, S. J., Holtz, B. A., & Michailides, T. J. (1996). Mating behavior in field populations of Monilinia fructicola in California. Mycologia, 88, 208–211.
Gell, I., Cubero, J., & Melgarejo, P. (2007). Two different PCR approaches for universal diagnosis of brown rot and identification of Monilinia spp. in stone fruit trees. Journal of Applied Microbiology, 103, 2629–2637.
Gell, I., De Cal, A., Torres, R., Usall, J., & Melgarejo, P. (2008). Relationship between the incidence of latent infections caused by Monilinia spp. and the incidence of brown rot of peach fruit: factors affecting latent infection. European Journal of Plant Pathology, 121, 487–498.
Gell, I., De Cal, A., Torres, R., Usall, J., & Melgarejo, P. (2009). Conidial density of Monilinia spp. on peach fruit surfaces in relation to the incidences of latent infections and brown rot. European Journal of Plant Pathology, 123, 415–424.
Grabke, A., Hu, M. J., Luo, C. X., Bryson, P. K., & Schnabel, G. (2011). First report of brown rot of apple caused by Monilinia fructicola in Germany. Plant Diseases, 95, 6.
Gril, T., Celar, F., Munda, A., Javornik, B., & Jakse, J. (2008). AFLP analysis of intraspecific variation between Monilinia laxa isolates from different hosts. Plant Diseases, 92, 1616–1624.
Hewitt, W. B., & Leach, L. D. (1939). Brown rot Sclerotinias ocurring in California and their distribution on stone fruits. Phytopathol., 29, 337–351.
Hilber-Bodmer, M., Knorst, V., Smits, T. H. M., & Patocchi, A. (2010). First report of brown rot caused by Monilinia fructicola on apricot in a Swiss orchard. Plant Diseases, 94, 5.
Holtz, B. A., Michailides, T. J., & Hong, C. X. (1998). Development of apothecia from stone fruit infected and stromatized by Monilinia fructicola in California. Plant Diseases, 82, 1375–1380.
Hu, M.-J., Cox, K. D., Schnabel, G., & Luo, C.-X. (2011). Monilinia species causing brown rot of peach in China. PLoS One, 6, e24990. doi:10.1371/journal.pone.0024990.
Larena, I., Torres, R., De Cal, A., Liñan, M., Melgarejo, P., Domenichini, P., et al. (2005). Biological control of postharvest brown rot (Monilinia spp.) of peaches by field applications of Epicoccum nigrum. Biological Control, 32, 305–310.
Lichou, J., Mandrin, J. F., Breniaux, D., Mercier, V., Giauque, P., Desbrus, D., et al. (2002). Une nouvelle moniliose. Phytoma, 547, 22–25.
Luo, Y., & Michailides, T. (2003). Threshold conditions that lead latent infection to prune fruit rot caused by Monilinia fructicola. Phytopathology, 93, 102–111.
Ma, Z. H., Yoshimura, M. A., & Michailides, T. J. (2003). Identification and characterization of Benzimidazole resistance in Monilinia fructicola from stone fruit orchards in California. Applied and Environmental Microbiology, 69, 7145–7152.
Meier, U., Graf, H., Hess, M., Kennel, W., Klose, R., Mappes, D., et al. (1994). Phänologische Entwick-lungsstadien des Kernobstes (Malus domestica Borkh. und Pyrus communis L.), des Steinobstes (Prunus-Arten), der Johannisbeere (Ribes-Arten) und der Erdbeere (Fragaria x ananassa Duch.). Nachrichtenbl. Deut. Pflanzenschutzd., 46, 141–153.
Munda, A., & Viršček Marn, M. (2010). First report of brown rot caused by Monilinia fructicola affecting peach orchards in Slovenia. Plant Diseases, 94, 9.
Ogawa, J. M., Manji, B. T., Bostock, R. M., Canez, V. M., & Bose, E. A. (1984). Detection and characterization of benomyl-resistant Monilinia laxa on apricots. Plant Diseases, 68, 29–31.
Pellegrino, C., Gullino, M. L., Garibaldi, A., & Spadaro, D. (2009). First report of brown rot of stone fruit caused by Monilinia fructicola in Italy. Plant Diseases, 93, 668.
Sauer, D. B., & Burroughs, R. (1986). Disinfection of seed surfaces with sodium-hypochlorite. Phytopathology, 76, 745–749.
Schnabel, G., Bryson, P. K., Bridges, W. C., & Brannen, P. M. (2004). Reduced sensitivity in Monilinia fructicola to propiconazole in Georgia and implications for disease management. Plant Disease, 88, 1000–1004.
Snedecor, G. W., & Cochram, W. G. (1980). Statistical methods (7th ed.). Ames: Iowa State University Press.
Van Leeuwen, G. C. M., Baayen, R. P., & Jeger, M. J. (2001). Pest risk assessment for the countries of the European Union (as PRA area) on Monilinia fructicola. Bulletin OEPP/EPPO Bulletin, 31(4), 481–487.
Villarino, M., Melgarejo, P., Usall, J., Segarra, J., & De Cal, A. (2010). Primary inoculum sources of Monilinia spp. in Spanish peach orchards and their relative importance in brown rot. Plant Diseases, 94, 1048–1054.
Villarino, M., Melgarejo, P., Usall, J., Segarra, J., Lamarca, N., & De Cal, A. (2012a). Secondary inoculum dynamics of Monilinia spp. and relationship to the incidence of postharvest brown rot in peaches and the weather conditions during the growing season. European Journal of Plant Pathology, 133, 585–598.
Villarino, M., Larena, I., Martinez, F., Melgarejo, P., & De Cal, A. (2012b). Analysis of genetic diversity in Monilinia fructicola from the Ebro Valley in Spain using ISSR and RAPD markers. European Journal of Plant Pathology, 132, 511–524.
Zehr, E. I. (1982). Control of brown rot in peach orchards. Plant Disease Report, 66, 1101–1105.
Zhong, Y. F., Zhang, Y. W., Chen, X. Y., Luo, Y., & Guo, L. Y. (2008). Overwintering of Monilinia fructicola in stone fruit orchards in northern China. Journal of Phytopathology, 156, 229–235.
Acknowledgments
This study was supported by grants RTA2005-00077-CO2, AGL2008-4396-CO2, and AGL2011-30472-CO2 from the Ministry of Economy and Competitive (Spain). B. Egüen received a scholarship from the Spanish Ministry of Economy and Competiveness. We thank R. Castillo and M.T. Morales-Clemente for their technical support, and the growers for their support and collaboration. The authors would like to acknowledge Dr. Arieh Bomzon, ConsulWrite (www.consulwrite.com) for his editorial assistance in preparing this manuscript.
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Villarino, M., Egüen, B., Lamarca, N. et al. Occurrence of Monilinia laxa and M. fructigena after introduction of M. fructicola in peach orchards in Spain. Eur J Plant Pathol 137, 835–845 (2013). https://doi.org/10.1007/s10658-013-0292-6
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DOI: https://doi.org/10.1007/s10658-013-0292-6