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Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia

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Abstract

Infection of fruit trees by Pseudomonas syringae is a potentially serious problem that may limit the establishment and sustained productivity of pome and stone fruit orchards in Serbia. To estimate possible diversity of Pseudomonas syringae fruit trees strains, we collected a set of strains in several areas of Serbia. The samples were taken from infected orchards with raspberry, plum, cherry, sour cherry, peach, pear and apple trees. Genetic diversity of P. syringae strains isolated from fruit trees was determined by using SpeI macrorestriction analysis of genomic DNAs by pulsed-field gel electrophoresis (PFGE) and REP-PCR. Molecular analysis showed that most of isolates had unique profiles, with the exception of isolates from plum and cherry that displayed profiles identical to each other and similar to P. syringae pv. morsprunorum. The study presented here clearly demonstrates the discriminative power of molecular techniques in enabling a detailed analysis of the genetic variations between strains of P. syringae from different pome and stone fruit hosts in Serbia.

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Acknowledgments

This work was supported by the Ministry of Education and Science, Republic of Serbia (Grants No. 173026 and 31018)

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Authors and Affiliations

  1. Institute for Plant Protection and the Environment, 11010, Belgrade, Serbia

    Žarko Ivanović, Svetlana Živković & Veljko Gavrilović

  2. Faculty of Biology, University of Belgrade, 11010, Belgrade, Serbia

    Slaviša Stanković & Djordje Fira

  3. Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444/a, P.O. Box 23, 11010, Belgrade, Serbia

    Milan Kojić & Djordje Fira

Authors
  1. Žarko Ivanović
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  2. Slaviša Stanković
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  3. Svetlana Živković
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  4. Veljko Gavrilović
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  5. Milan Kojić
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  6. Djordje Fira
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Correspondence to Djordje Fira.

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Ivanović, Ž., Stanković, S., Živković, S. et al. Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia. Eur J Plant Pathol 134, 191–203 (2012). https://doi.org/10.1007/s10658-012-9978-4

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  • DOI: https://doi.org/10.1007/s10658-012-9978-4

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